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Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment

Beta cell failure is one of the most important features of type 2 diabetes mellitus (T2DM). High‐density lipoprotein (HDL) has been proposed to improve β‐cell function. However, the mechanisms involved in this process are still poorly understood. The aim of this study was to investigate the contribu...

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Autores principales: Brulhart‐Meynet, Marie‐Claude, Thomas, Aurélien, Sidibé, Jonathan, Visentin, Florian, Dusaulcy, Rodolphe, Schwitzgebel, Valérie, Pataky, Zoltan, Philippe, Jacques, Vuilleumier, Nicolas, James, Richard W., Gosmain, Yvan, Frias, Miguel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995544/
https://www.ncbi.nlm.nih.gov/pubmed/33769715
http://dx.doi.org/10.14814/phy2.14786
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author Brulhart‐Meynet, Marie‐Claude
Thomas, Aurélien
Sidibé, Jonathan
Visentin, Florian
Dusaulcy, Rodolphe
Schwitzgebel, Valérie
Pataky, Zoltan
Philippe, Jacques
Vuilleumier, Nicolas
James, Richard W.
Gosmain, Yvan
Frias, Miguel A.
author_facet Brulhart‐Meynet, Marie‐Claude
Thomas, Aurélien
Sidibé, Jonathan
Visentin, Florian
Dusaulcy, Rodolphe
Schwitzgebel, Valérie
Pataky, Zoltan
Philippe, Jacques
Vuilleumier, Nicolas
James, Richard W.
Gosmain, Yvan
Frias, Miguel A.
author_sort Brulhart‐Meynet, Marie‐Claude
collection PubMed
description Beta cell failure is one of the most important features of type 2 diabetes mellitus (T2DM). High‐density lipoprotein (HDL) has been proposed to improve β‐cell function. However, the mechanisms involved in this process are still poorly understood. The aim of this study was to investigate the contribution of sphingosine‐1‐phosphate (S1P) in the impact of HDL treatment on insulin secretion by pancreatic β‐cells and to determine its mechanisms. Primary cultures of β‐cells isolated from rat were treated with or without HDL in the presence or absence of S1P pathway inhibitors and insulin secretion response was analyzed. The S1P content of HDL (HDL‐S1P) isolated from T2DM patients was analyzed and correlated to the HDL‐induced insulin secretion. The expression of genes involved in the biosynthesis of the insulin was also evaluated. HDL as well as S1P treatment enhanced glucose‐stimulated insulin secretion (GSIS). In HDL isolated from T2DM patients, while HDL‐S1P was strongly correlated to its pro‐secretory capacity (r = 0.633, p = 0.005), HDL‐cholesterol and apolipoprotein AI levels were not. HDL‐induced GSIS was blocked by the S1P1/3 antagonist but not by the S1P2 antagonist, and was also accompanied by increased intracellular S1P in β‐cells. We also observed that HDL improved GSIS without significant changes in expression levels of insulin biosynthesis genes. Our present study highlights the importance HDL‐S1P in GSIS in T2DM patients and demonstrates that HDL induces insulin secretion by a process involving both intra‐ and extra‐cellular sources of S1P independently of an effect on insulin biosynthesis genes.
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spelling pubmed-79955442021-03-30 Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment Brulhart‐Meynet, Marie‐Claude Thomas, Aurélien Sidibé, Jonathan Visentin, Florian Dusaulcy, Rodolphe Schwitzgebel, Valérie Pataky, Zoltan Philippe, Jacques Vuilleumier, Nicolas James, Richard W. Gosmain, Yvan Frias, Miguel A. Physiol Rep Original Articles Beta cell failure is one of the most important features of type 2 diabetes mellitus (T2DM). High‐density lipoprotein (HDL) has been proposed to improve β‐cell function. However, the mechanisms involved in this process are still poorly understood. The aim of this study was to investigate the contribution of sphingosine‐1‐phosphate (S1P) in the impact of HDL treatment on insulin secretion by pancreatic β‐cells and to determine its mechanisms. Primary cultures of β‐cells isolated from rat were treated with or without HDL in the presence or absence of S1P pathway inhibitors and insulin secretion response was analyzed. The S1P content of HDL (HDL‐S1P) isolated from T2DM patients was analyzed and correlated to the HDL‐induced insulin secretion. The expression of genes involved in the biosynthesis of the insulin was also evaluated. HDL as well as S1P treatment enhanced glucose‐stimulated insulin secretion (GSIS). In HDL isolated from T2DM patients, while HDL‐S1P was strongly correlated to its pro‐secretory capacity (r = 0.633, p = 0.005), HDL‐cholesterol and apolipoprotein AI levels were not. HDL‐induced GSIS was blocked by the S1P1/3 antagonist but not by the S1P2 antagonist, and was also accompanied by increased intracellular S1P in β‐cells. We also observed that HDL improved GSIS without significant changes in expression levels of insulin biosynthesis genes. Our present study highlights the importance HDL‐S1P in GSIS in T2DM patients and demonstrates that HDL induces insulin secretion by a process involving both intra‐ and extra‐cellular sources of S1P independently of an effect on insulin biosynthesis genes. John Wiley and Sons Inc. 2021-03-26 /pmc/articles/PMC7995544/ /pubmed/33769715 http://dx.doi.org/10.14814/phy2.14786 Text en © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Brulhart‐Meynet, Marie‐Claude
Thomas, Aurélien
Sidibé, Jonathan
Visentin, Florian
Dusaulcy, Rodolphe
Schwitzgebel, Valérie
Pataky, Zoltan
Philippe, Jacques
Vuilleumier, Nicolas
James, Richard W.
Gosmain, Yvan
Frias, Miguel A.
Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
title Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
title_full Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
title_fullStr Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
title_full_unstemmed Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
title_short Sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
title_sort sphingosine‐1‐phosphate as a key player of insulin secretion induced by high‐density lipoprotein treatment
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995544/
https://www.ncbi.nlm.nih.gov/pubmed/33769715
http://dx.doi.org/10.14814/phy2.14786
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