Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here...

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Detalles Bibliográficos
Autores principales: Xian, Quanxiang, Qiu, Zhihai, Kala, Shashwati, Guo, Jinghui, Zhu, Jiejun, Wong, Kin Fung, Guo, Sean Shu Yi, Zhu, Ting, Hou, Xuandi, Sun, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995654/
https://www.ncbi.nlm.nih.gov/pubmed/33796870
http://dx.doi.org/10.1016/j.xpro.2021.100393
Descripción
Sumario:Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).

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