Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here...

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Autores principales: Xian, Quanxiang, Qiu, Zhihai, Kala, Shashwati, Guo, Jinghui, Zhu, Jiejun, Wong, Kin Fung, Guo, Sean Shu Yi, Zhu, Ting, Hou, Xuandi, Sun, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995654/
https://www.ncbi.nlm.nih.gov/pubmed/33796870
http://dx.doi.org/10.1016/j.xpro.2021.100393
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author Xian, Quanxiang
Qiu, Zhihai
Kala, Shashwati
Guo, Jinghui
Zhu, Jiejun
Wong, Kin Fung
Guo, Sean Shu Yi
Zhu, Ting
Hou, Xuandi
Sun, Lei
author_facet Xian, Quanxiang
Qiu, Zhihai
Kala, Shashwati
Guo, Jinghui
Zhu, Jiejun
Wong, Kin Fung
Guo, Sean Shu Yi
Zhu, Ting
Hou, Xuandi
Sun, Lei
author_sort Xian, Quanxiang
collection PubMed
description Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).
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spelling pubmed-79956542021-03-31 Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound Xian, Quanxiang Qiu, Zhihai Kala, Shashwati Guo, Jinghui Zhu, Jiejun Wong, Kin Fung Guo, Sean Shu Yi Zhu, Ting Hou, Xuandi Sun, Lei STAR Protoc Protocol Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020). Elsevier 2021-03-16 /pmc/articles/PMC7995654/ /pubmed/33796870 http://dx.doi.org/10.1016/j.xpro.2021.100393 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xian, Quanxiang
Qiu, Zhihai
Kala, Shashwati
Guo, Jinghui
Zhu, Jiejun
Wong, Kin Fung
Guo, Sean Shu Yi
Zhu, Ting
Hou, Xuandi
Sun, Lei
Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
title Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
title_full Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
title_fullStr Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
title_full_unstemmed Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
title_short Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
title_sort protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995654/
https://www.ncbi.nlm.nih.gov/pubmed/33796870
http://dx.doi.org/10.1016/j.xpro.2021.100393
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