Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress

Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to reg...

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Detalles Bibliográficos
Autores principales: Abutaleb, Nadia O., Truskey, George A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995664/
https://www.ncbi.nlm.nih.gov/pubmed/33796871
http://dx.doi.org/10.1016/j.xpro.2021.100394
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author Abutaleb, Nadia O.
Truskey, George A.
author_facet Abutaleb, Nadia O.
Truskey, George A.
author_sort Abutaleb, Nadia O.
collection PubMed
description Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).
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spelling pubmed-79956642021-03-31 Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress Abutaleb, Nadia O. Truskey, George A. STAR Protoc Protocol Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020). Elsevier 2021-03-17 /pmc/articles/PMC7995664/ /pubmed/33796871 http://dx.doi.org/10.1016/j.xpro.2021.100394 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Abutaleb, Nadia O.
Truskey, George A.
Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress
title Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress
title_full Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress
title_fullStr Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress
title_full_unstemmed Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress
title_short Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress
title_sort differentiation and characterization of human ipsc-derived vascular endothelial cells under physiological shear stress
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995664/
https://www.ncbi.nlm.nih.gov/pubmed/33796871
http://dx.doi.org/10.1016/j.xpro.2021.100394
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