Cargando…

Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human g...

Descripción completa

Detalles Bibliográficos
Autores principales: Raethong, Nachon, Nakphaichit, Massalin, Suratannon, Narissara, Sathitkowitchai, Witida, Weerapakorn, Wanlapa, Keawsompong, Suttipun, Vongsangnak, Wanwipa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996147/
https://www.ncbi.nlm.nih.gov/pubmed/33668840
http://dx.doi.org/10.3390/genes12030331
_version_ 1783670051040133120
author Raethong, Nachon
Nakphaichit, Massalin
Suratannon, Narissara
Sathitkowitchai, Witida
Weerapakorn, Wanlapa
Keawsompong, Suttipun
Vongsangnak, Wanwipa
author_facet Raethong, Nachon
Nakphaichit, Massalin
Suratannon, Narissara
Sathitkowitchai, Witida
Weerapakorn, Wanlapa
Keawsompong, Suttipun
Vongsangnak, Wanwipa
author_sort Raethong, Nachon
collection PubMed
description The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country.
format Online
Article
Text
id pubmed-7996147
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79961472021-03-27 Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults Raethong, Nachon Nakphaichit, Massalin Suratannon, Narissara Sathitkowitchai, Witida Weerapakorn, Wanlapa Keawsompong, Suttipun Vongsangnak, Wanwipa Genes (Basel) Article The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country. MDPI 2021-02-25 /pmc/articles/PMC7996147/ /pubmed/33668840 http://dx.doi.org/10.3390/genes12030331 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Raethong, Nachon
Nakphaichit, Massalin
Suratannon, Narissara
Sathitkowitchai, Witida
Weerapakorn, Wanlapa
Keawsompong, Suttipun
Vongsangnak, Wanwipa
Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
title Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
title_full Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
title_fullStr Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
title_full_unstemmed Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
title_short Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
title_sort analysis of human gut microbiome: taxonomy and metabolic functions in thai adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996147/
https://www.ncbi.nlm.nih.gov/pubmed/33668840
http://dx.doi.org/10.3390/genes12030331
work_keys_str_mv AT raethongnachon analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults
AT nakphaichitmassalin analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults
AT suratannonnarissara analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults
AT sathitkowitchaiwitida analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults
AT weerapakornwanlapa analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults
AT keawsompongsuttipun analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults
AT vongsangnakwanwipa analysisofhumangutmicrobiometaxonomyandmetabolicfunctionsinthaiadults