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Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults
The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human g...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996147/ https://www.ncbi.nlm.nih.gov/pubmed/33668840 http://dx.doi.org/10.3390/genes12030331 |
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author | Raethong, Nachon Nakphaichit, Massalin Suratannon, Narissara Sathitkowitchai, Witida Weerapakorn, Wanlapa Keawsompong, Suttipun Vongsangnak, Wanwipa |
author_facet | Raethong, Nachon Nakphaichit, Massalin Suratannon, Narissara Sathitkowitchai, Witida Weerapakorn, Wanlapa Keawsompong, Suttipun Vongsangnak, Wanwipa |
author_sort | Raethong, Nachon |
collection | PubMed |
description | The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country. |
format | Online Article Text |
id | pubmed-7996147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79961472021-03-27 Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults Raethong, Nachon Nakphaichit, Massalin Suratannon, Narissara Sathitkowitchai, Witida Weerapakorn, Wanlapa Keawsompong, Suttipun Vongsangnak, Wanwipa Genes (Basel) Article The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country. MDPI 2021-02-25 /pmc/articles/PMC7996147/ /pubmed/33668840 http://dx.doi.org/10.3390/genes12030331 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Raethong, Nachon Nakphaichit, Massalin Suratannon, Narissara Sathitkowitchai, Witida Weerapakorn, Wanlapa Keawsompong, Suttipun Vongsangnak, Wanwipa Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
title | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
title_full | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
title_fullStr | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
title_full_unstemmed | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
title_short | Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults |
title_sort | analysis of human gut microbiome: taxonomy and metabolic functions in thai adults |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996147/ https://www.ncbi.nlm.nih.gov/pubmed/33668840 http://dx.doi.org/10.3390/genes12030331 |
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