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The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression
Streptococcus pneumoniae two-component regulatory systems (TCSs) are important systems that perceive and respond to various host environmental stimuli. In this study, we have explored the role of TCS09 on gene expression and phenotypic alterations in S. pneumoniae D39. Our comparative transcriptomic...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996280/ https://www.ncbi.nlm.nih.gov/pubmed/33668344 http://dx.doi.org/10.3390/microorganisms9030468 |
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author | Hirschmann, Stephanie Gómez-Mejia, Alejandro Mäder, Ulrike Karsunke, Julia Driesch, Dominik Rohde, Manfred Häussler, Susanne Burchhardt, Gerhard Hammerschmidt, Sven |
author_facet | Hirschmann, Stephanie Gómez-Mejia, Alejandro Mäder, Ulrike Karsunke, Julia Driesch, Dominik Rohde, Manfred Häussler, Susanne Burchhardt, Gerhard Hammerschmidt, Sven |
author_sort | Hirschmann, Stephanie |
collection | PubMed |
description | Streptococcus pneumoniae two-component regulatory systems (TCSs) are important systems that perceive and respond to various host environmental stimuli. In this study, we have explored the role of TCS09 on gene expression and phenotypic alterations in S. pneumoniae D39. Our comparative transcriptomic analyses identified 67 differently expressed genes in total. Among those, agaR and the aga operon involved in galactose metabolism showed the highest changes. Intriguingly, the encapsulated and nonencapsulated hk09-mutants showed significant growth defects under nutrient-defined conditions, in particular with galactose as a carbon source. Phenotypic analyses revealed alterations in the morphology of the nonencapsulated hk09- and tcs09-mutants, whereas the encapsulated hk09- and tcs09-mutants produced higher amounts of capsule. Interestingly, the encapsulated D39∆hk09 showed only the opaque colony morphology, while the D39∆rr09- and D39∆tcs09-mutants had a higher proportion of transparent variants. The phenotypic variations of D39ΔcpsΔhk09 and D39ΔcpsΔtcs09 are in accordance with their higher numbers of outer membrane vesicles, higher sensitivity against Triton X-100 induced autolysis, and lower resistance against oxidative stress. In conclusion, these results indicate the importance of TCS09 for pneumococcal metabolic fitness and resistance against oxidative stress by regulating the carbohydrate metabolism and thereby, most likely indirectly, the cell wall integrity and amount of capsular polysaccharide. |
format | Online Article Text |
id | pubmed-7996280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79962802021-03-27 The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression Hirschmann, Stephanie Gómez-Mejia, Alejandro Mäder, Ulrike Karsunke, Julia Driesch, Dominik Rohde, Manfred Häussler, Susanne Burchhardt, Gerhard Hammerschmidt, Sven Microorganisms Article Streptococcus pneumoniae two-component regulatory systems (TCSs) are important systems that perceive and respond to various host environmental stimuli. In this study, we have explored the role of TCS09 on gene expression and phenotypic alterations in S. pneumoniae D39. Our comparative transcriptomic analyses identified 67 differently expressed genes in total. Among those, agaR and the aga operon involved in galactose metabolism showed the highest changes. Intriguingly, the encapsulated and nonencapsulated hk09-mutants showed significant growth defects under nutrient-defined conditions, in particular with galactose as a carbon source. Phenotypic analyses revealed alterations in the morphology of the nonencapsulated hk09- and tcs09-mutants, whereas the encapsulated hk09- and tcs09-mutants produced higher amounts of capsule. Interestingly, the encapsulated D39∆hk09 showed only the opaque colony morphology, while the D39∆rr09- and D39∆tcs09-mutants had a higher proportion of transparent variants. The phenotypic variations of D39ΔcpsΔhk09 and D39ΔcpsΔtcs09 are in accordance with their higher numbers of outer membrane vesicles, higher sensitivity against Triton X-100 induced autolysis, and lower resistance against oxidative stress. In conclusion, these results indicate the importance of TCS09 for pneumococcal metabolic fitness and resistance against oxidative stress by regulating the carbohydrate metabolism and thereby, most likely indirectly, the cell wall integrity and amount of capsular polysaccharide. MDPI 2021-02-24 /pmc/articles/PMC7996280/ /pubmed/33668344 http://dx.doi.org/10.3390/microorganisms9030468 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Hirschmann, Stephanie Gómez-Mejia, Alejandro Mäder, Ulrike Karsunke, Julia Driesch, Dominik Rohde, Manfred Häussler, Susanne Burchhardt, Gerhard Hammerschmidt, Sven The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression |
title | The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression |
title_full | The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression |
title_fullStr | The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression |
title_full_unstemmed | The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression |
title_short | The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression |
title_sort | two-component system 09 regulates pneumococcal carbohydrate metabolism and capsule expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996280/ https://www.ncbi.nlm.nih.gov/pubmed/33668344 http://dx.doi.org/10.3390/microorganisms9030468 |
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