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Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile

Arsenic (As), a semimetal toxic for humans, is commonly associated with serious health problems. The most common form of massive and chronic exposure to As is through consumption of contaminated drinking water. This study aimed to isolate an As resistant bacterial strain to characterize its ability...

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Autores principales: Herrera, Constanza, Moraga, Ruben, Bustamante, Brian, Vilo, Claudia, Aguayo, Paulina, Valenzuela, Cristian, Smith, Carlos T., Yáñez, Jorge, Guzmán-Fierro, Victor, Roeckel, Marlene, Campos, Víctor L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996500/
https://www.ncbi.nlm.nih.gov/pubmed/33668956
http://dx.doi.org/10.3390/microorganisms9030483
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author Herrera, Constanza
Moraga, Ruben
Bustamante, Brian
Vilo, Claudia
Aguayo, Paulina
Valenzuela, Cristian
Smith, Carlos T.
Yáñez, Jorge
Guzmán-Fierro, Victor
Roeckel, Marlene
Campos, Víctor L.
author_facet Herrera, Constanza
Moraga, Ruben
Bustamante, Brian
Vilo, Claudia
Aguayo, Paulina
Valenzuela, Cristian
Smith, Carlos T.
Yáñez, Jorge
Guzmán-Fierro, Victor
Roeckel, Marlene
Campos, Víctor L.
author_sort Herrera, Constanza
collection PubMed
description Arsenic (As), a semimetal toxic for humans, is commonly associated with serious health problems. The most common form of massive and chronic exposure to As is through consumption of contaminated drinking water. This study aimed to isolate an As resistant bacterial strain to characterize its ability to oxidize As (III) when immobilized in an activated carbon batch bioreactor and to evaluate its potential to be used in biological treatments to remediate As contaminated waters. The diversity of bacterial communities from sediments of the As-rich Camarones River, Atacama Desert, Chile, was evaluated by Illumina sequencing. Dominant taxonomic groups (>1%) isolated were affiliated with Proteobacteria and Firmicutes. A high As-resistant bacterium was selected (Pseudomonas migulae VC-19 strain) and the presence of aio gene in it was investigated. Arsenite detoxification activity by this bacterial strain was determined by HPLC/HG/AAS. Particularly when immobilized on activated carbon, P. migulae VC-19 showed high rates of As(III) conversion (100% oxidized after 36 h of incubation). To the best of our knowledge, this is the first report of a P. migulae arsenite oxidizing strain that is promising for biotechnological application in the treatment of arsenic contaminated waters.
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spelling pubmed-79965002021-03-27 Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile Herrera, Constanza Moraga, Ruben Bustamante, Brian Vilo, Claudia Aguayo, Paulina Valenzuela, Cristian Smith, Carlos T. Yáñez, Jorge Guzmán-Fierro, Victor Roeckel, Marlene Campos, Víctor L. Microorganisms Article Arsenic (As), a semimetal toxic for humans, is commonly associated with serious health problems. The most common form of massive and chronic exposure to As is through consumption of contaminated drinking water. This study aimed to isolate an As resistant bacterial strain to characterize its ability to oxidize As (III) when immobilized in an activated carbon batch bioreactor and to evaluate its potential to be used in biological treatments to remediate As contaminated waters. The diversity of bacterial communities from sediments of the As-rich Camarones River, Atacama Desert, Chile, was evaluated by Illumina sequencing. Dominant taxonomic groups (>1%) isolated were affiliated with Proteobacteria and Firmicutes. A high As-resistant bacterium was selected (Pseudomonas migulae VC-19 strain) and the presence of aio gene in it was investigated. Arsenite detoxification activity by this bacterial strain was determined by HPLC/HG/AAS. Particularly when immobilized on activated carbon, P. migulae VC-19 showed high rates of As(III) conversion (100% oxidized after 36 h of incubation). To the best of our knowledge, this is the first report of a P. migulae arsenite oxidizing strain that is promising for biotechnological application in the treatment of arsenic contaminated waters. MDPI 2021-02-25 /pmc/articles/PMC7996500/ /pubmed/33668956 http://dx.doi.org/10.3390/microorganisms9030483 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Herrera, Constanza
Moraga, Ruben
Bustamante, Brian
Vilo, Claudia
Aguayo, Paulina
Valenzuela, Cristian
Smith, Carlos T.
Yáñez, Jorge
Guzmán-Fierro, Victor
Roeckel, Marlene
Campos, Víctor L.
Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile
title Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile
title_full Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile
title_fullStr Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile
title_full_unstemmed Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile
title_short Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile
title_sort characterization of arsenite-oxidizing bacteria isolated from arsenic-rich sediments, atacama desert, chile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996500/
https://www.ncbi.nlm.nih.gov/pubmed/33668956
http://dx.doi.org/10.3390/microorganisms9030483
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