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Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis

To elucidate defense mechanisms of Piper nigrum against fusariosis, an experiment based on co-inoculation of arbuscular mycorrhizal fungi (AMF) and Fusarium solani f. sp. piperis was performed. Variations in secondary metabolism in plants infected with F. solani f. sp. piperis (FUS) and co-inoculate...

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Autores principales: Trindade, Rafaela, Almeida, Laís, Xavier, Luciana, Andrade, Eloisa Helena, Maia, José Guilherme, Mello, Andréa, Setzer, William N., Ramos, Alessandra, da Silva, Joyce Kelly R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996571/
https://www.ncbi.nlm.nih.gov/pubmed/33669088
http://dx.doi.org/10.3390/microorganisms9030484
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author Trindade, Rafaela
Almeida, Laís
Xavier, Luciana
Andrade, Eloisa Helena
Maia, José Guilherme
Mello, Andréa
Setzer, William N.
Ramos, Alessandra
da Silva, Joyce Kelly R.
author_facet Trindade, Rafaela
Almeida, Laís
Xavier, Luciana
Andrade, Eloisa Helena
Maia, José Guilherme
Mello, Andréa
Setzer, William N.
Ramos, Alessandra
da Silva, Joyce Kelly R.
author_sort Trindade, Rafaela
collection PubMed
description To elucidate defense mechanisms of Piper nigrum against fusariosis, an experiment based on co-inoculation of arbuscular mycorrhizal fungi (AMF) and Fusarium solani f. sp. piperis was performed. Variations in secondary metabolism in plants infected with F. solani f. sp. piperis (FUS) and co-inoculated with AMFs and F. solani (AMF + FUS) were monitored at 7- and 21-days post inoculations (dpi). The pathogen induced a decrease in oxygenated sesquiterpenes (82.0–77.4%), and changes in the concentrations of the main compounds, α-muurolene, α-muurolol, and 2E-hexenal in the leaves. It was observed that the concentration of 2E-hexenal decreased at 7 dpi, α-muurolene decreased at 21 dpi, and α-muurolol increased at 21 dpi. There was a prevalence of sesquiterpene and monoterpene hydrocarbons in the roots, such as β-caryophyllene, δ-elemene, and limonene. The infection and co-inoculation induced greater production of phenolics in the roots at 7 dpi. The enzymatic activity of phenylalanine ammonia-lyase decreased in the leaves at 21 dpi and in the roots on both days, while the lipoxygenase activity decreased only in the roots at 21 dpi. The results demonstrated that co-inoculation with AMFs and F. solani induces changes in the defense metabolism of P. nigrum, but it is not efficient in the biocontrol of fusariosis during the evaluated period.
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spelling pubmed-79965712021-03-27 Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis Trindade, Rafaela Almeida, Laís Xavier, Luciana Andrade, Eloisa Helena Maia, José Guilherme Mello, Andréa Setzer, William N. Ramos, Alessandra da Silva, Joyce Kelly R. Microorganisms Article To elucidate defense mechanisms of Piper nigrum against fusariosis, an experiment based on co-inoculation of arbuscular mycorrhizal fungi (AMF) and Fusarium solani f. sp. piperis was performed. Variations in secondary metabolism in plants infected with F. solani f. sp. piperis (FUS) and co-inoculated with AMFs and F. solani (AMF + FUS) were monitored at 7- and 21-days post inoculations (dpi). The pathogen induced a decrease in oxygenated sesquiterpenes (82.0–77.4%), and changes in the concentrations of the main compounds, α-muurolene, α-muurolol, and 2E-hexenal in the leaves. It was observed that the concentration of 2E-hexenal decreased at 7 dpi, α-muurolene decreased at 21 dpi, and α-muurolol increased at 21 dpi. There was a prevalence of sesquiterpene and monoterpene hydrocarbons in the roots, such as β-caryophyllene, δ-elemene, and limonene. The infection and co-inoculation induced greater production of phenolics in the roots at 7 dpi. The enzymatic activity of phenylalanine ammonia-lyase decreased in the leaves at 21 dpi and in the roots on both days, while the lipoxygenase activity decreased only in the roots at 21 dpi. The results demonstrated that co-inoculation with AMFs and F. solani induces changes in the defense metabolism of P. nigrum, but it is not efficient in the biocontrol of fusariosis during the evaluated period. MDPI 2021-02-25 /pmc/articles/PMC7996571/ /pubmed/33669088 http://dx.doi.org/10.3390/microorganisms9030484 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Trindade, Rafaela
Almeida, Laís
Xavier, Luciana
Andrade, Eloisa Helena
Maia, José Guilherme
Mello, Andréa
Setzer, William N.
Ramos, Alessandra
da Silva, Joyce Kelly R.
Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis
title Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis
title_full Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis
title_fullStr Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis
title_full_unstemmed Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis
title_short Influence on Secondary Metabolism of Piper nigrum L. by Co-Inoculation with Arbuscular Mycorrhizal Fungi and Fusarium solani f. sp. piperis
title_sort influence on secondary metabolism of piper nigrum l. by co-inoculation with arbuscular mycorrhizal fungi and fusarium solani f. sp. piperis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996571/
https://www.ncbi.nlm.nih.gov/pubmed/33669088
http://dx.doi.org/10.3390/microorganisms9030484
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