Cargando…

The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene

Triticum turgidum and T. timopheevii are two tetraploid wheat species sharing T. urartu as a common ancestor, and domesticated accessions from both of these allopolyploids exhibit nonbrittle rachis (i.e., nonshattering spikes). We previously described the loss-of-function mutations in the Brittle Ra...

Descripción completa

Detalles Bibliográficos
Autores principales: Nave, Moran, Taş, Mihriban, Raupp, John, Tiwari, Vijay K., Ozkan, Hakan, Poland, Jesse, Hale, Iago, Komatsuda, Takao, Distelfeld, Assaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996576/
https://www.ncbi.nlm.nih.gov/pubmed/33668927
http://dx.doi.org/10.3390/genes12030338
_version_ 1783670135005904896
author Nave, Moran
Taş, Mihriban
Raupp, John
Tiwari, Vijay K.
Ozkan, Hakan
Poland, Jesse
Hale, Iago
Komatsuda, Takao
Distelfeld, Assaf
author_facet Nave, Moran
Taş, Mihriban
Raupp, John
Tiwari, Vijay K.
Ozkan, Hakan
Poland, Jesse
Hale, Iago
Komatsuda, Takao
Distelfeld, Assaf
author_sort Nave, Moran
collection PubMed
description Triticum turgidum and T. timopheevii are two tetraploid wheat species sharing T. urartu as a common ancestor, and domesticated accessions from both of these allopolyploids exhibit nonbrittle rachis (i.e., nonshattering spikes). We previously described the loss-of-function mutations in the Brittle Rachis 1 genes BTR1-A and BTR1-B in the A and B subgenomes, respectively, that are responsible for this most visible domestication trait in T. turgidum. Resequencing of a large panel of wild and domesticated T. turgidum accessions subsequently led to the identification of the two progenitor haplotypes of the btr1-A and btr1-B domesticated alleles. Here, we extended the haplotype analysis to other T. turgidum subspecies and to the BTR1 homologues in the related T. timopheevii species. Our results showed that all the domesticated wheat subspecies within T. turgidum share common BTR1-A and BTR1-B haplotypes, confirming their common origin. In T. timopheevii, however, we identified a novel loss-of-function btr1-A allele underlying a partially brittle spike phenotype. This novel recessive allele appeared fixed within the pool of domesticated Timopheev’s wheat but was also carried by one wild timopheevii accession exhibiting partial brittleness. The promoter region for BTR1-B could not be amplified in any T. timopheevii accessions with any T. turgidum primer combination, exemplifying the gene-level distance between the two species. Altogether, our results support the concept of independent domestication processes for the two polyploid, wheat-related species.
format Online
Article
Text
id pubmed-7996576
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79965762021-03-27 The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene Nave, Moran Taş, Mihriban Raupp, John Tiwari, Vijay K. Ozkan, Hakan Poland, Jesse Hale, Iago Komatsuda, Takao Distelfeld, Assaf Genes (Basel) Article Triticum turgidum and T. timopheevii are two tetraploid wheat species sharing T. urartu as a common ancestor, and domesticated accessions from both of these allopolyploids exhibit nonbrittle rachis (i.e., nonshattering spikes). We previously described the loss-of-function mutations in the Brittle Rachis 1 genes BTR1-A and BTR1-B in the A and B subgenomes, respectively, that are responsible for this most visible domestication trait in T. turgidum. Resequencing of a large panel of wild and domesticated T. turgidum accessions subsequently led to the identification of the two progenitor haplotypes of the btr1-A and btr1-B domesticated alleles. Here, we extended the haplotype analysis to other T. turgidum subspecies and to the BTR1 homologues in the related T. timopheevii species. Our results showed that all the domesticated wheat subspecies within T. turgidum share common BTR1-A and BTR1-B haplotypes, confirming their common origin. In T. timopheevii, however, we identified a novel loss-of-function btr1-A allele underlying a partially brittle spike phenotype. This novel recessive allele appeared fixed within the pool of domesticated Timopheev’s wheat but was also carried by one wild timopheevii accession exhibiting partial brittleness. The promoter region for BTR1-B could not be amplified in any T. timopheevii accessions with any T. turgidum primer combination, exemplifying the gene-level distance between the two species. Altogether, our results support the concept of independent domestication processes for the two polyploid, wheat-related species. MDPI 2021-02-25 /pmc/articles/PMC7996576/ /pubmed/33668927 http://dx.doi.org/10.3390/genes12030338 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Nave, Moran
Taş, Mihriban
Raupp, John
Tiwari, Vijay K.
Ozkan, Hakan
Poland, Jesse
Hale, Iago
Komatsuda, Takao
Distelfeld, Assaf
The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
title The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
title_full The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
title_fullStr The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
title_full_unstemmed The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
title_short The Independent Domestication of Timopheev’s Wheat: Insights from Haplotype Analysis of the Brittle rachis 1 (BTR1-A) Gene
title_sort independent domestication of timopheev’s wheat: insights from haplotype analysis of the brittle rachis 1 (btr1-a) gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996576/
https://www.ncbi.nlm.nih.gov/pubmed/33668927
http://dx.doi.org/10.3390/genes12030338
work_keys_str_mv AT navemoran theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT tasmihriban theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT rauppjohn theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT tiwarivijayk theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT ozkanhakan theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT polandjesse theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT haleiago theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT komatsudatakao theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT distelfeldassaf theindependentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT navemoran independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT tasmihriban independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT rauppjohn independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT tiwarivijayk independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT ozkanhakan independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT polandjesse independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT haleiago independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT komatsudatakao independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene
AT distelfeldassaf independentdomesticationoftimopheevswheatinsightsfromhaplotypeanalysisofthebrittlerachis1btr1agene