Cargando…

Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment

Background: The 2014–2016 Ebola outbreak in West Africa recapitulated that nosocomial spread of Ebola virus could occur and that health care workers were at particular risk including notable cases in Europe and North America. These instances highlighted the need for centers to better prepare for pot...

Descripción completa

Detalles Bibliográficos
Autores principales: Biondi, Mia J., Garnett, Lauren, Bello, Alexander, Funk, Duane, Poliquin, Philippe Guillaume, Jones, Shane, Tierney, Kevin, Tran, Kaylie, Kozak, Robert A., Leung, Anders, Grolla, Allen, Nakamura, Cory, Soule, Geoff, Ranadheera, Charlene, Hagan, Mable, Dhaliwal, Amrinder, Kobasa, Darwyn, Falzarano, Darryl, Bovendo, Hugues Fausther, Feldmann, Heinz, Kesselman, Murray, Hansen, Gregory, Gren, Jason, Mortimer, Todd, Racine, Trina, Deschambault, Yvon, Edmonds, Jocelyn, Aminian, Sam, Saurette, Ray, Allan, Mark, Rondeau, Lauren, Huynh, John, Hadder, Sharron, Press, Christy, DeGraff, Christine, Kucas, Stephanie, Kubay, Julie, Azanarsky, Kim, Cook, Bradley W. M., Hancock, BJ, Kumar, Anand, Soni, Reeni, Schantz, Daryl, McKitrick, Jarrid, Warner, Bryce, Griffin, Bryan D., Qiu, Xiangguo, Kobinger, Gary P., Safronetz, Dave, Wood, Heidi, Stein, Derek R., Cutts, Todd, Pickering, Brad, Kenny, James, Theriault, Steven, Menec, Liam, Vendramelli, Robert, Higgins, Sean, Banadyga, Logan, Liu, Guodong, Rahim, Md Niaz, Kasloff, Samantha, Sloan, Angela, He, Shihua, Tailor, Nikesh, Albietz, Alixandra, Wong, Gary, Gray, Michael, Feldmann, Friederike, Marzi, Andrea, Risi, George, Strong, James E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996731/
https://www.ncbi.nlm.nih.gov/pubmed/33652895
http://dx.doi.org/10.3390/microorganisms9030498
_version_ 1783670166730571776
author Biondi, Mia J.
Garnett, Lauren
Bello, Alexander
Funk, Duane
Poliquin, Philippe Guillaume
Jones, Shane
Tierney, Kevin
Tran, Kaylie
Kozak, Robert A.
Leung, Anders
Grolla, Allen
Nakamura, Cory
Soule, Geoff
Ranadheera, Charlene
Hagan, Mable
Dhaliwal, Amrinder
Kobasa, Darwyn
Falzarano, Darryl
Bovendo, Hugues Fausther
Feldmann, Heinz
Kesselman, Murray
Hansen, Gregory
Gren, Jason
Mortimer, Todd
Racine, Trina
Deschambault, Yvon
Edmonds, Jocelyn
Aminian, Sam
Saurette, Ray
Allan, Mark
Rondeau, Lauren
Huynh, John
Hadder, Sharron
Press, Christy
DeGraff, Christine
Kucas, Stephanie
Kubay, Julie
Azanarsky, Kim
Cook, Bradley W. M.
Hancock, BJ
Kumar, Anand
Soni, Reeni
Schantz, Daryl
McKitrick, Jarrid
Warner, Bryce
Griffin, Bryan D.
Qiu, Xiangguo
Kobinger, Gary P.
Safronetz, Dave
Wood, Heidi
Stein, Derek R.
Cutts, Todd
Pickering, Brad
Kenny, James
Theriault, Steven
Menec, Liam
Vendramelli, Robert
Higgins, Sean
Banadyga, Logan
Liu, Guodong
Rahim, Md Niaz
Kasloff, Samantha
Sloan, Angela
He, Shihua
Tailor, Nikesh
Albietz, Alixandra
Wong, Gary
Gray, Michael
Feldmann, Friederike
Marzi, Andrea
Risi, George
Strong, James E.
author_facet Biondi, Mia J.
Garnett, Lauren
Bello, Alexander
Funk, Duane
Poliquin, Philippe Guillaume
Jones, Shane
Tierney, Kevin
Tran, Kaylie
Kozak, Robert A.
Leung, Anders
Grolla, Allen
Nakamura, Cory
Soule, Geoff
Ranadheera, Charlene
Hagan, Mable
Dhaliwal, Amrinder
Kobasa, Darwyn
Falzarano, Darryl
Bovendo, Hugues Fausther
Feldmann, Heinz
Kesselman, Murray
Hansen, Gregory
Gren, Jason
Mortimer, Todd
Racine, Trina
Deschambault, Yvon
Edmonds, Jocelyn
Aminian, Sam
Saurette, Ray
Allan, Mark
Rondeau, Lauren
Huynh, John
Hadder, Sharron
Press, Christy
DeGraff, Christine
Kucas, Stephanie
Kubay, Julie
Azanarsky, Kim
Cook, Bradley W. M.
Hancock, BJ
Kumar, Anand
Soni, Reeni
Schantz, Daryl
McKitrick, Jarrid
Warner, Bryce
Griffin, Bryan D.
Qiu, Xiangguo
Kobinger, Gary P.
Safronetz, Dave
Wood, Heidi
Stein, Derek R.
Cutts, Todd
Pickering, Brad
Kenny, James
Theriault, Steven
Menec, Liam
Vendramelli, Robert
Higgins, Sean
Banadyga, Logan
Liu, Guodong
Rahim, Md Niaz
Kasloff, Samantha
Sloan, Angela
He, Shihua
Tailor, Nikesh
Albietz, Alixandra
Wong, Gary
Gray, Michael
Feldmann, Friederike
Marzi, Andrea
Risi, George
Strong, James E.
author_sort Biondi, Mia J.
collection PubMed
description Background: The 2014–2016 Ebola outbreak in West Africa recapitulated that nosocomial spread of Ebola virus could occur and that health care workers were at particular risk including notable cases in Europe and North America. These instances highlighted the need for centers to better prepare for potential Ebola virus cases; including understanding how the virus spreads and which interventions pose the greatest risk. Methods: We created a fully equipped intensive care unit (ICU), within a Biosafety Level 4 (BSL4) laboratory, and infected multiple sedated non-human primates (NHPs) with Ebola virus. While providing bedside care, we sampled blood, urine, and gastric residuals; as well as buccal, ocular, nasal, rectal, and skin swabs, to assess the risks associated with routine care. We also assessed the physical environment at end-point. Results: Although viral RNA was detectable in blood as early as three days post-infection, it was not detectable in the urine, gastric fluid, or swabs until late-stage disease. While droplet spread and fomite contamination were present on a few of the surfaces that were routinely touched while providing care in the ICU for the infected animal, these may have been abrogated through good routine hygiene practices. Conclusions: Overall this study has helped further our understanding of which procedures may pose the highest risk to healthcare providers and provides temporal evidence of this over the clinical course of disease.
format Online
Article
Text
id pubmed-7996731
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79967312021-03-27 Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment Biondi, Mia J. Garnett, Lauren Bello, Alexander Funk, Duane Poliquin, Philippe Guillaume Jones, Shane Tierney, Kevin Tran, Kaylie Kozak, Robert A. Leung, Anders Grolla, Allen Nakamura, Cory Soule, Geoff Ranadheera, Charlene Hagan, Mable Dhaliwal, Amrinder Kobasa, Darwyn Falzarano, Darryl Bovendo, Hugues Fausther Feldmann, Heinz Kesselman, Murray Hansen, Gregory Gren, Jason Mortimer, Todd Racine, Trina Deschambault, Yvon Edmonds, Jocelyn Aminian, Sam Saurette, Ray Allan, Mark Rondeau, Lauren Huynh, John Hadder, Sharron Press, Christy DeGraff, Christine Kucas, Stephanie Kubay, Julie Azanarsky, Kim Cook, Bradley W. M. Hancock, BJ Kumar, Anand Soni, Reeni Schantz, Daryl McKitrick, Jarrid Warner, Bryce Griffin, Bryan D. Qiu, Xiangguo Kobinger, Gary P. Safronetz, Dave Wood, Heidi Stein, Derek R. Cutts, Todd Pickering, Brad Kenny, James Theriault, Steven Menec, Liam Vendramelli, Robert Higgins, Sean Banadyga, Logan Liu, Guodong Rahim, Md Niaz Kasloff, Samantha Sloan, Angela He, Shihua Tailor, Nikesh Albietz, Alixandra Wong, Gary Gray, Michael Feldmann, Friederike Marzi, Andrea Risi, George Strong, James E. Microorganisms Article Background: The 2014–2016 Ebola outbreak in West Africa recapitulated that nosocomial spread of Ebola virus could occur and that health care workers were at particular risk including notable cases in Europe and North America. These instances highlighted the need for centers to better prepare for potential Ebola virus cases; including understanding how the virus spreads and which interventions pose the greatest risk. Methods: We created a fully equipped intensive care unit (ICU), within a Biosafety Level 4 (BSL4) laboratory, and infected multiple sedated non-human primates (NHPs) with Ebola virus. While providing bedside care, we sampled blood, urine, and gastric residuals; as well as buccal, ocular, nasal, rectal, and skin swabs, to assess the risks associated with routine care. We also assessed the physical environment at end-point. Results: Although viral RNA was detectable in blood as early as three days post-infection, it was not detectable in the urine, gastric fluid, or swabs until late-stage disease. While droplet spread and fomite contamination were present on a few of the surfaces that were routinely touched while providing care in the ICU for the infected animal, these may have been abrogated through good routine hygiene practices. Conclusions: Overall this study has helped further our understanding of which procedures may pose the highest risk to healthcare providers and provides temporal evidence of this over the clinical course of disease. MDPI 2021-02-26 /pmc/articles/PMC7996731/ /pubmed/33652895 http://dx.doi.org/10.3390/microorganisms9030498 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Biondi, Mia J.
Garnett, Lauren
Bello, Alexander
Funk, Duane
Poliquin, Philippe Guillaume
Jones, Shane
Tierney, Kevin
Tran, Kaylie
Kozak, Robert A.
Leung, Anders
Grolla, Allen
Nakamura, Cory
Soule, Geoff
Ranadheera, Charlene
Hagan, Mable
Dhaliwal, Amrinder
Kobasa, Darwyn
Falzarano, Darryl
Bovendo, Hugues Fausther
Feldmann, Heinz
Kesselman, Murray
Hansen, Gregory
Gren, Jason
Mortimer, Todd
Racine, Trina
Deschambault, Yvon
Edmonds, Jocelyn
Aminian, Sam
Saurette, Ray
Allan, Mark
Rondeau, Lauren
Huynh, John
Hadder, Sharron
Press, Christy
DeGraff, Christine
Kucas, Stephanie
Kubay, Julie
Azanarsky, Kim
Cook, Bradley W. M.
Hancock, BJ
Kumar, Anand
Soni, Reeni
Schantz, Daryl
McKitrick, Jarrid
Warner, Bryce
Griffin, Bryan D.
Qiu, Xiangguo
Kobinger, Gary P.
Safronetz, Dave
Wood, Heidi
Stein, Derek R.
Cutts, Todd
Pickering, Brad
Kenny, James
Theriault, Steven
Menec, Liam
Vendramelli, Robert
Higgins, Sean
Banadyga, Logan
Liu, Guodong
Rahim, Md Niaz
Kasloff, Samantha
Sloan, Angela
He, Shihua
Tailor, Nikesh
Albietz, Alixandra
Wong, Gary
Gray, Michael
Feldmann, Friederike
Marzi, Andrea
Risi, George
Strong, James E.
Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment
title Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment
title_full Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment
title_fullStr Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment
title_full_unstemmed Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment
title_short Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment
title_sort characterization of ebola virus risk to bedside providers in an intensive care environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996731/
https://www.ncbi.nlm.nih.gov/pubmed/33652895
http://dx.doi.org/10.3390/microorganisms9030498
work_keys_str_mv AT biondimiaj characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT garnettlauren characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT belloalexander characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT funkduane characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT poliquinphilippeguillaume characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT jonesshane characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT tierneykevin characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT trankaylie characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kozakroberta characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT leunganders characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT grollaallen characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT nakamuracory characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT soulegeoff characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT ranadheeracharlene characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT haganmable characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT dhaliwalamrinder characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kobasadarwyn characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT falzaranodarryl characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT bovendohuguesfausther characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT feldmannheinz characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kesselmanmurray characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT hansengregory characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT grenjason characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT mortimertodd characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT racinetrina characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT deschambaultyvon characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT edmondsjocelyn characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT aminiansam characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT sauretteray characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT allanmark characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT rondeaulauren characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT huynhjohn characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT haddersharron characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT presschristy characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT degraffchristine characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kucasstephanie characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kubayjulie characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT azanarskykim characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT cookbradleywm characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT hancockbj characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kumaranand characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT sonireeni characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT schantzdaryl characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT mckitrickjarrid characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT warnerbryce characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT griffinbryand characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT qiuxiangguo characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kobingergaryp characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT safronetzdave characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT woodheidi characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT steinderekr characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT cuttstodd characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT pickeringbrad characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kennyjames characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT theriaultsteven characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT menecliam characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT vendramellirobert characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT higginssean characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT banadygalogan characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT liuguodong characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT rahimmdniaz characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT kasloffsamantha characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT sloanangela characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT heshihua characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT tailornikesh characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT albietzalixandra characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT wonggary characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT graymichael characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT feldmannfriederike characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT marziandrea characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT risigeorge characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment
AT strongjamese characterizationofebolavirusrisktobedsideprovidersinanintensivecareenvironment