Skeletal Development and Deformities in Tench (Tinca tinca): From Basic knowledge to Regular Monitoring Procedure

SIMPLE SUMMARY: Fish skeletal development and incidence of skeletal deformities are important factors to warrant aquaculture success. Skeletal deformities reduce fish viability, growth, and feed efficiency but also degrade the consumer’s perception of aquaculture products. Some skeletal deformities would also decrease animal wellbeing. Tench (Tinca tinca) is a freshwater species cultured in ponds, highly demanded in particular regions of Europe and a promising species for aquaculture diversification. Determining the onset of the different skeletal structures may help fish farmers to adapt and improve rearing practices (e.g., water temperature, feeds composition, etc.) to decrease the incidence of skeletal deformities. At the same time, monitoring the incidence of skeletal deformities represents a valuable decision-making tool to improve efficient use of facilities and resources. ABSTRACT: Skeletal deformities reduce fish viability, growth, wellbeing, and feed efficiency but also degrade the consumer’s perception of aquaculture products. Herein, the skeletal development and the incidence of skeletal deformities in tench (Tinca tinca) reared in semi-extensive conditions has been described in detail for the first time. Larval skeletons were assessed through an acid-free double-staining procedure in 157 individuals, while 274 specimens at the juvenile stage were evaluated through X-ray analysis. The first skeletal structures to be formed were those related with breathing and feeding activities (e.g., Meckel’s cartilage and opercula) and were visible in larvae of 4 mm of standard length (SL). The axial skeleton was fully ossified in larvae of 12–17 mm of SL, and the caudal fin complex in larvae with 17–26 mm of SL. At the larval stage, no upper-jaw or opercula deformities were observed, while a low incidence (1–9%) of other severe deformities in the heads of the fish (e.g., lower-jaw deformities) were reported. The incidence of vertebral deformities in tench reared in natural ponds was considerable in larvae (54%) and juveniles (52%). Vertebral deformities (fusion and compression) were the most common deformities found in tench larvae (approximately 30%) and vertebral shape deformity in juveniles (around 10%), being mainly located in the caudal region. Thus, a regular monitoring of the skeletal deformities in tench might help to identify better rearing protocols and improve product quality sold at markets. Characterizing the skeletal development not only in semi-extensive systems such as artificial and natural ponds but also under intensive rearing conditions, seems vital for a sustainable and profitable European tench aquaculture.