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Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia

A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used...

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Autores principales: Kabali, Emmanuel, Pandey, Girja Shanker, Munyeme, Musso, Kapila, Penjaninge, Mukubesa, Andrew Nalishuwa, Ndebe, Joseph, Muma, John Bwalya, Mubita, Charles, Muleya, Walter, Muonga, Elizabeth Muligisa, Mitoma, Shuya, Hang’ombe, Bernard Mudenda, Wiratsudakul, Anuwat, Ngan, Mai Thi, Elhanafy, Eslam, Daous, Hala El, Huyen, Nguyen Thi, Yamazaki, Wataru, Okabayashi, Tamaki, Abe, Maiku, Norimine, Junzo, Sekiguchi, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996741/
https://www.ncbi.nlm.nih.gov/pubmed/33652871
http://dx.doi.org/10.3390/antibiotics10030238
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author Kabali, Emmanuel
Pandey, Girja Shanker
Munyeme, Musso
Kapila, Penjaninge
Mukubesa, Andrew Nalishuwa
Ndebe, Joseph
Muma, John Bwalya
Mubita, Charles
Muleya, Walter
Muonga, Elizabeth Muligisa
Mitoma, Shuya
Hang’ombe, Bernard Mudenda
Wiratsudakul, Anuwat
Ngan, Mai Thi
Elhanafy, Eslam
Daous, Hala El
Huyen, Nguyen Thi
Yamazaki, Wataru
Okabayashi, Tamaki
Abe, Maiku
Norimine, Junzo
Sekiguchi, Satoshi
author_facet Kabali, Emmanuel
Pandey, Girja Shanker
Munyeme, Musso
Kapila, Penjaninge
Mukubesa, Andrew Nalishuwa
Ndebe, Joseph
Muma, John Bwalya
Mubita, Charles
Muleya, Walter
Muonga, Elizabeth Muligisa
Mitoma, Shuya
Hang’ombe, Bernard Mudenda
Wiratsudakul, Anuwat
Ngan, Mai Thi
Elhanafy, Eslam
Daous, Hala El
Huyen, Nguyen Thi
Yamazaki, Wataru
Okabayashi, Tamaki
Abe, Maiku
Norimine, Junzo
Sekiguchi, Satoshi
author_sort Kabali, Emmanuel
collection PubMed
description A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife–livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern.
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spelling pubmed-79967412021-03-27 Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia Kabali, Emmanuel Pandey, Girja Shanker Munyeme, Musso Kapila, Penjaninge Mukubesa, Andrew Nalishuwa Ndebe, Joseph Muma, John Bwalya Mubita, Charles Muleya, Walter Muonga, Elizabeth Muligisa Mitoma, Shuya Hang’ombe, Bernard Mudenda Wiratsudakul, Anuwat Ngan, Mai Thi Elhanafy, Eslam Daous, Hala El Huyen, Nguyen Thi Yamazaki, Wataru Okabayashi, Tamaki Abe, Maiku Norimine, Junzo Sekiguchi, Satoshi Antibiotics (Basel) Article A cross-sectional study was used to identify and assess prevalence and phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling was used to select wildlife and livestock based on similarities in behavior, grazing habits and close interactions with humans. Isolates (n = 66) from fecal samples collected between April and August 2018 (n = 84) were examined following modified protocols for bacteria isolation, biochemical identification, molecular detection, phylogenetic analysis, and antimicrobial susceptibility testing by disc diffusion method. Data were analyzed using R software, Genetyx ver.12 and Mega 6. Using Applied Profile Index 20E kit for biochemical identification, polymerase chain reaction assay and sequencing, sixty-six isolates were identified to species level, of which Escherichia coli (72.7%, 48/66), E. fergusonii (1.5%, 1/66), Shigella sonnei (22.7%, 14/66), Sh. flexinerri (1.5%, 1/66) and Enterobacteriaceae bacterium (1.5%, 1/66), and their relationships were illustrated in a phylogenetic tree. Phenotypic antimicrobial resistance or intermediate sensitivity expression to at least one antimicrobial agent was detected in 89.6% of the E. coli, and 73.3% of the Shigella isolates. The E. coli isolates exhibited the highest resistance rates to ampicillin (27%), ceftazidime (14.3%), cefotaxime (9.5%), and kanamycin (9.5%). Multidrug resistance (MDR) was detected in 18.8% of E. coli isolates while only 13.3% Shigella isolates showed MDR. The MDR was detected among isolates from impala and ostrich (wild animals in which no antimicrobial treatment was used), and in isolates from cattle, pigs, and goats (domesticated animals). This study indicates the possible transmission of drug-resistant microorganisms between animals cohabiting at the wildlife–livestock interface. It emphasizes the need for further investigation of the role of wildlife in the development and transmission of AMR, which is an issue of global concern. MDPI 2021-02-26 /pmc/articles/PMC7996741/ /pubmed/33652871 http://dx.doi.org/10.3390/antibiotics10030238 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Kabali, Emmanuel
Pandey, Girja Shanker
Munyeme, Musso
Kapila, Penjaninge
Mukubesa, Andrew Nalishuwa
Ndebe, Joseph
Muma, John Bwalya
Mubita, Charles
Muleya, Walter
Muonga, Elizabeth Muligisa
Mitoma, Shuya
Hang’ombe, Bernard Mudenda
Wiratsudakul, Anuwat
Ngan, Mai Thi
Elhanafy, Eslam
Daous, Hala El
Huyen, Nguyen Thi
Yamazaki, Wataru
Okabayashi, Tamaki
Abe, Maiku
Norimine, Junzo
Sekiguchi, Satoshi
Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia
title Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia
title_full Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia
title_fullStr Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia
title_full_unstemmed Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia
title_short Identification of Escherichia coli and Related Enterobacteriaceae and Examination of Their Phenotypic Antimicrobial Resistance Patterns: A Pilot Study at A Wildlife–Livestock Interface in Lusaka, Zambia
title_sort identification of escherichia coli and related enterobacteriaceae and examination of their phenotypic antimicrobial resistance patterns: a pilot study at a wildlife–livestock interface in lusaka, zambia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996741/
https://www.ncbi.nlm.nih.gov/pubmed/33652871
http://dx.doi.org/10.3390/antibiotics10030238
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