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Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018

Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminan...

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Autores principales: Cichon, Nicole, Eiden, Martin, Schulz, Jana, Günther, Anne, Wysocki, Patrick, Holicki, Cora M., Borgwardt, Joachim, Gaede, Wolfgang, Groschup, Martin H., Ziegler, Ute
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996813/
https://www.ncbi.nlm.nih.gov/pubmed/33652882
http://dx.doi.org/10.3390/v13030370
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author Cichon, Nicole
Eiden, Martin
Schulz, Jana
Günther, Anne
Wysocki, Patrick
Holicki, Cora M.
Borgwardt, Joachim
Gaede, Wolfgang
Groschup, Martin H.
Ziegler, Ute
author_facet Cichon, Nicole
Eiden, Martin
Schulz, Jana
Günther, Anne
Wysocki, Patrick
Holicki, Cora M.
Borgwardt, Joachim
Gaede, Wolfgang
Groschup, Martin H.
Ziegler, Ute
author_sort Cichon, Nicole
collection PubMed
description Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminant species. We have, therefore, conducted a follow-up study in ruminants from Saxony-Anhalt, the most affected region in Eastern Germany. A total of 325 blood samples from apparently healthy sheep, goats, and cattle were tested using a BATV-specific qRT-PCR and SNT. Even though viral RNA was not detected, the presence of antibodies was confirmed in the sera of all three species: sheep (16.5%), goats (18.3%), and cattle (41.4%). Sera were further analyzed by a glycoprotein Gc-based indirect ELISA to evaluate Gc-derived antibodies as a basis for a new serological test for BATV infections. Interestingly, the presence of neutralizing antibodies was not directly linked to the presence of BATV Gc antibodies. Overall, our results illustrate the high frequency of BATV infections in ruminants in Eastern Germany.
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spelling pubmed-79968132021-03-27 Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018 Cichon, Nicole Eiden, Martin Schulz, Jana Günther, Anne Wysocki, Patrick Holicki, Cora M. Borgwardt, Joachim Gaede, Wolfgang Groschup, Martin H. Ziegler, Ute Viruses Article Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminant species. We have, therefore, conducted a follow-up study in ruminants from Saxony-Anhalt, the most affected region in Eastern Germany. A total of 325 blood samples from apparently healthy sheep, goats, and cattle were tested using a BATV-specific qRT-PCR and SNT. Even though viral RNA was not detected, the presence of antibodies was confirmed in the sera of all three species: sheep (16.5%), goats (18.3%), and cattle (41.4%). Sera were further analyzed by a glycoprotein Gc-based indirect ELISA to evaluate Gc-derived antibodies as a basis for a new serological test for BATV infections. Interestingly, the presence of neutralizing antibodies was not directly linked to the presence of BATV Gc antibodies. Overall, our results illustrate the high frequency of BATV infections in ruminants in Eastern Germany. MDPI 2021-02-26 /pmc/articles/PMC7996813/ /pubmed/33652882 http://dx.doi.org/10.3390/v13030370 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Cichon, Nicole
Eiden, Martin
Schulz, Jana
Günther, Anne
Wysocki, Patrick
Holicki, Cora M.
Borgwardt, Joachim
Gaede, Wolfgang
Groschup, Martin H.
Ziegler, Ute
Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
title Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
title_full Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
title_fullStr Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
title_full_unstemmed Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
title_short Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018
title_sort serological and molecular investigation of batai virus infections in ruminants from the state of saxony-anhalt, germany, 2018
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996813/
https://www.ncbi.nlm.nih.gov/pubmed/33652882
http://dx.doi.org/10.3390/v13030370
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