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Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
Urinary antigen tests (UATs) are often used to diagnose Legionnaires’ disease as they are rapid and easy to perform on readily obtainable urine samples without the need for specialized skills compared to conventional methods. Recently developed automated readers for UATs may provide objective result...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996842/ https://www.ncbi.nlm.nih.gov/pubmed/33652772 http://dx.doi.org/10.3390/microorganisms9030493 |
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author | Wong, Alicia Y. W. Johnsson, Alexander T. A. Iversen, Aina Athlin, Simon Özenci, Volkan |
author_facet | Wong, Alicia Y. W. Johnsson, Alexander T. A. Iversen, Aina Athlin, Simon Özenci, Volkan |
author_sort | Wong, Alicia Y. W. |
collection | PubMed |
description | Urinary antigen tests (UATs) are often used to diagnose Legionnaires’ disease as they are rapid and easy to perform on readily obtainable urine samples without the need for specialized skills compared to conventional methods. Recently developed automated readers for UATs may provide objective results interpretation, especially in cases of weak result bands. Using 53 defined patient urine samples, we evaluated the performance of the BinaxNOW Legionella Antigen Card (Abbott), ImmuView S. pneumoniae and Legionella (SSI Diagnostica), STANDARD F Legionella Ag FIA (SD Biosensor), and Sofia Legionella FIA (Quidel) simultaneously with their respective automated readers. Automatic and visual interpretation of result bands were also compared for the immunochromatography-based BinaxNOW and ImmuView UATs. Overall sensitivity and specificity of Legionella UATs were 53.9–61.5% and 90.0–94.9%, respectively. All four UATs successfully detected all samples from L. pneumophila serogroup 1-positive patients, but most failed to detect samples for Legionella spp., or other serogroups. Automatic results interpretation of results was found to be mostly concordant with visual results reading. In conclusion, the performance of the four UATs were similar to each other in the detection of Legionella urinary antigen with no major difference between automated or visual results reading. |
format | Online Article Text |
id | pubmed-7996842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79968422021-03-27 Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen Wong, Alicia Y. W. Johnsson, Alexander T. A. Iversen, Aina Athlin, Simon Özenci, Volkan Microorganisms Communication Urinary antigen tests (UATs) are often used to diagnose Legionnaires’ disease as they are rapid and easy to perform on readily obtainable urine samples without the need for specialized skills compared to conventional methods. Recently developed automated readers for UATs may provide objective results interpretation, especially in cases of weak result bands. Using 53 defined patient urine samples, we evaluated the performance of the BinaxNOW Legionella Antigen Card (Abbott), ImmuView S. pneumoniae and Legionella (SSI Diagnostica), STANDARD F Legionella Ag FIA (SD Biosensor), and Sofia Legionella FIA (Quidel) simultaneously with their respective automated readers. Automatic and visual interpretation of result bands were also compared for the immunochromatography-based BinaxNOW and ImmuView UATs. Overall sensitivity and specificity of Legionella UATs were 53.9–61.5% and 90.0–94.9%, respectively. All four UATs successfully detected all samples from L. pneumophila serogroup 1-positive patients, but most failed to detect samples for Legionella spp., or other serogroups. Automatic results interpretation of results was found to be mostly concordant with visual results reading. In conclusion, the performance of the four UATs were similar to each other in the detection of Legionella urinary antigen with no major difference between automated or visual results reading. MDPI 2021-02-26 /pmc/articles/PMC7996842/ /pubmed/33652772 http://dx.doi.org/10.3390/microorganisms9030493 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Communication Wong, Alicia Y. W. Johnsson, Alexander T. A. Iversen, Aina Athlin, Simon Özenci, Volkan Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen |
title | Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen |
title_full | Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen |
title_fullStr | Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen |
title_full_unstemmed | Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen |
title_short | Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen |
title_sort | evaluation of four lateral flow assays for the detection of legionella urinary antigen |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996842/ https://www.ncbi.nlm.nih.gov/pubmed/33652772 http://dx.doi.org/10.3390/microorganisms9030493 |
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