Cargando…

Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen

Urinary antigen tests (UATs) are often used to diagnose Legionnaires’ disease as they are rapid and easy to perform on readily obtainable urine samples without the need for specialized skills compared to conventional methods. Recently developed automated readers for UATs may provide objective result...

Descripción completa

Detalles Bibliográficos
Autores principales: Wong, Alicia Y. W., Johnsson, Alexander T. A., Iversen, Aina, Athlin, Simon, Özenci, Volkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996842/
https://www.ncbi.nlm.nih.gov/pubmed/33652772
http://dx.doi.org/10.3390/microorganisms9030493
_version_ 1783670192733159424
author Wong, Alicia Y. W.
Johnsson, Alexander T. A.
Iversen, Aina
Athlin, Simon
Özenci, Volkan
author_facet Wong, Alicia Y. W.
Johnsson, Alexander T. A.
Iversen, Aina
Athlin, Simon
Özenci, Volkan
author_sort Wong, Alicia Y. W.
collection PubMed
description Urinary antigen tests (UATs) are often used to diagnose Legionnaires’ disease as they are rapid and easy to perform on readily obtainable urine samples without the need for specialized skills compared to conventional methods. Recently developed automated readers for UATs may provide objective results interpretation, especially in cases of weak result bands. Using 53 defined patient urine samples, we evaluated the performance of the BinaxNOW Legionella Antigen Card (Abbott), ImmuView S. pneumoniae and Legionella (SSI Diagnostica), STANDARD F Legionella Ag FIA (SD Biosensor), and Sofia Legionella FIA (Quidel) simultaneously with their respective automated readers. Automatic and visual interpretation of result bands were also compared for the immunochromatography-based BinaxNOW and ImmuView UATs. Overall sensitivity and specificity of Legionella UATs were 53.9–61.5% and 90.0–94.9%, respectively. All four UATs successfully detected all samples from L. pneumophila serogroup 1-positive patients, but most failed to detect samples for Legionella spp., or other serogroups. Automatic results interpretation of results was found to be mostly concordant with visual results reading. In conclusion, the performance of the four UATs were similar to each other in the detection of Legionella urinary antigen with no major difference between automated or visual results reading.
format Online
Article
Text
id pubmed-7996842
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79968422021-03-27 Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen Wong, Alicia Y. W. Johnsson, Alexander T. A. Iversen, Aina Athlin, Simon Özenci, Volkan Microorganisms Communication Urinary antigen tests (UATs) are often used to diagnose Legionnaires’ disease as they are rapid and easy to perform on readily obtainable urine samples without the need for specialized skills compared to conventional methods. Recently developed automated readers for UATs may provide objective results interpretation, especially in cases of weak result bands. Using 53 defined patient urine samples, we evaluated the performance of the BinaxNOW Legionella Antigen Card (Abbott), ImmuView S. pneumoniae and Legionella (SSI Diagnostica), STANDARD F Legionella Ag FIA (SD Biosensor), and Sofia Legionella FIA (Quidel) simultaneously with their respective automated readers. Automatic and visual interpretation of result bands were also compared for the immunochromatography-based BinaxNOW and ImmuView UATs. Overall sensitivity and specificity of Legionella UATs were 53.9–61.5% and 90.0–94.9%, respectively. All four UATs successfully detected all samples from L. pneumophila serogroup 1-positive patients, but most failed to detect samples for Legionella spp., or other serogroups. Automatic results interpretation of results was found to be mostly concordant with visual results reading. In conclusion, the performance of the four UATs were similar to each other in the detection of Legionella urinary antigen with no major difference between automated or visual results reading. MDPI 2021-02-26 /pmc/articles/PMC7996842/ /pubmed/33652772 http://dx.doi.org/10.3390/microorganisms9030493 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Communication
Wong, Alicia Y. W.
Johnsson, Alexander T. A.
Iversen, Aina
Athlin, Simon
Özenci, Volkan
Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
title Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
title_full Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
title_fullStr Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
title_full_unstemmed Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
title_short Evaluation of Four Lateral Flow Assays for the Detection of Legionella Urinary Antigen
title_sort evaluation of four lateral flow assays for the detection of legionella urinary antigen
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996842/
https://www.ncbi.nlm.nih.gov/pubmed/33652772
http://dx.doi.org/10.3390/microorganisms9030493
work_keys_str_mv AT wongaliciayw evaluationoffourlateralflowassaysforthedetectionoflegionellaurinaryantigen
AT johnssonalexanderta evaluationoffourlateralflowassaysforthedetectionoflegionellaurinaryantigen
AT iversenaina evaluationoffourlateralflowassaysforthedetectionoflegionellaurinaryantigen
AT athlinsimon evaluationoffourlateralflowassaysforthedetectionoflegionellaurinaryantigen
AT ozencivolkan evaluationoffourlateralflowassaysforthedetectionoflegionellaurinaryantigen