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Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto de Medicina Tropical
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997665/ https://www.ncbi.nlm.nih.gov/pubmed/33787744 http://dx.doi.org/10.1590/S1678-9946202163024 |
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author | Muñoz-Gamba, Andrew S. Laiton-Donato, Katherine Perdomo-Balaguera, Erick Castro, Lyda R. Usme-Ciro, José A. Parra-Henao, Gabriel |
author_facet | Muñoz-Gamba, Andrew S. Laiton-Donato, Katherine Perdomo-Balaguera, Erick Castro, Lyda R. Usme-Ciro, José A. Parra-Henao, Gabriel |
author_sort | Muñoz-Gamba, Andrew S. |
collection | PubMed |
description | A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases. |
format | Online Article Text |
id | pubmed-7997665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-79976652021-04-06 Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest Muñoz-Gamba, Andrew S. Laiton-Donato, Katherine Perdomo-Balaguera, Erick Castro, Lyda R. Usme-Ciro, José A. Parra-Henao, Gabriel Rev Inst Med Trop Sao Paulo Original Article A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases. Instituto de Medicina Tropical 2021-03-24 /pmc/articles/PMC7997665/ /pubmed/33787744 http://dx.doi.org/10.1590/S1678-9946202163024 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Muñoz-Gamba, Andrew S. Laiton-Donato, Katherine Perdomo-Balaguera, Erick Castro, Lyda R. Usme-Ciro, José A. Parra-Henao, Gabriel Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest |
title | Molecular characterization of mosquitoes (Diptera: Culicidae) from
the Colombian rainforest |
title_full | Molecular characterization of mosquitoes (Diptera: Culicidae) from
the Colombian rainforest |
title_fullStr | Molecular characterization of mosquitoes (Diptera: Culicidae) from
the Colombian rainforest |
title_full_unstemmed | Molecular characterization of mosquitoes (Diptera: Culicidae) from
the Colombian rainforest |
title_short | Molecular characterization of mosquitoes (Diptera: Culicidae) from
the Colombian rainforest |
title_sort | molecular characterization of mosquitoes (diptera: culicidae) from
the colombian rainforest |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997665/ https://www.ncbi.nlm.nih.gov/pubmed/33787744 http://dx.doi.org/10.1590/S1678-9946202163024 |
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