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Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest

A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in...

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Autores principales: Muñoz-Gamba, Andrew S., Laiton-Donato, Katherine, Perdomo-Balaguera, Erick, Castro, Lyda R., Usme-Ciro, José A., Parra-Henao, Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997665/
https://www.ncbi.nlm.nih.gov/pubmed/33787744
http://dx.doi.org/10.1590/S1678-9946202163024
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author Muñoz-Gamba, Andrew S.
Laiton-Donato, Katherine
Perdomo-Balaguera, Erick
Castro, Lyda R.
Usme-Ciro, José A.
Parra-Henao, Gabriel
author_facet Muñoz-Gamba, Andrew S.
Laiton-Donato, Katherine
Perdomo-Balaguera, Erick
Castro, Lyda R.
Usme-Ciro, José A.
Parra-Henao, Gabriel
author_sort Muñoz-Gamba, Andrew S.
collection PubMed
description A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases.
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spelling pubmed-79976652021-04-06 Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest Muñoz-Gamba, Andrew S. Laiton-Donato, Katherine Perdomo-Balaguera, Erick Castro, Lyda R. Usme-Ciro, José A. Parra-Henao, Gabriel Rev Inst Med Trop Sao Paulo Original Article A few studies have carried out the taxonomic and molecular characterization of sylvatic mosquito species in Latin America, where some species have been incriminated as vectors for arboviruses and parasites transmission. The present study reports the molecular characterization of mosquito species in the Sierra Nevada de Santa Marta, a natural ecosystem in the Northern coast of Colombia. Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed using the neighbor-joining method, with the Kimura-2-Parameters model. Aedes serratus , Psorophora ferox , Johnbelkinia ulopus , Sabethes chloropterus , Sabethes cyaneus , Wyeomyia aporonoma , Wyeomyia pseudopecten , Wyeomyia ulocoma and Wyeomyia luteoventralis were identified. We assessed the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed the identification at the species level. Classical and molecular taxonomy demonstrated to be useful and complementary when morphological characteristics are not well preserved, or the taxonomic group is not represented in public molecular databases. Instituto de Medicina Tropical 2021-03-24 /pmc/articles/PMC7997665/ /pubmed/33787744 http://dx.doi.org/10.1590/S1678-9946202163024 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Muñoz-Gamba, Andrew S.
Laiton-Donato, Katherine
Perdomo-Balaguera, Erick
Castro, Lyda R.
Usme-Ciro, José A.
Parra-Henao, Gabriel
Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_full Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_fullStr Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_full_unstemmed Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_short Molecular characterization of mosquitoes (Diptera: Culicidae) from the Colombian rainforest
title_sort molecular characterization of mosquitoes (diptera: culicidae) from the colombian rainforest
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997665/
https://www.ncbi.nlm.nih.gov/pubmed/33787744
http://dx.doi.org/10.1590/S1678-9946202163024
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