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Adhesion strength and contractility enable metastatic cells to become adurotactic
Significant changes in cell stiffness, contractility, and adhesion, i.e., mechanotype, are observed during a variety of biological processes. Whether cell mechanics merely change as a side effect of or driver for biological processes is still unclear. Here, we sort genotypically similar metastatic c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997775/ https://www.ncbi.nlm.nih.gov/pubmed/33691109 http://dx.doi.org/10.1016/j.celrep.2021.108816 |
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author | Yeoman, Benjamin Shatkin, Gabriel Beri, Pranjali Banisadr, Afsheen Katira, Parag Engler, Adam J. |
author_facet | Yeoman, Benjamin Shatkin, Gabriel Beri, Pranjali Banisadr, Afsheen Katira, Parag Engler, Adam J. |
author_sort | Yeoman, Benjamin |
collection | PubMed |
description | Significant changes in cell stiffness, contractility, and adhesion, i.e., mechanotype, are observed during a variety of biological processes. Whether cell mechanics merely change as a side effect of or driver for biological processes is still unclear. Here, we sort genotypically similar metastatic cancer cells into strongly adherent (SA) versus weakly adherent (WA) phenotypes to study how contractility and adhesion differences alter the ability of cells to sense and respond to gradients in material stiffness. We observe that SA cells migrate up a stiffness gradient, or durotax, while WA cells largely ignore the gradient, i.e., adurotax. Biophysical modeling and experimental validation suggest that differences in cell migration and durotaxis between weakly and strongly adherent cells are driven by differences in intra-cellular actomyosin activity. These results provide a direct relationship between cell phenotype and durotaxis and suggest how, unlike other senescent cells, metastatic cancer cells navigate against stiffness gradients. |
format | Online Article Text |
id | pubmed-7997775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-79977752021-03-27 Adhesion strength and contractility enable metastatic cells to become adurotactic Yeoman, Benjamin Shatkin, Gabriel Beri, Pranjali Banisadr, Afsheen Katira, Parag Engler, Adam J. Cell Rep Article Significant changes in cell stiffness, contractility, and adhesion, i.e., mechanotype, are observed during a variety of biological processes. Whether cell mechanics merely change as a side effect of or driver for biological processes is still unclear. Here, we sort genotypically similar metastatic cancer cells into strongly adherent (SA) versus weakly adherent (WA) phenotypes to study how contractility and adhesion differences alter the ability of cells to sense and respond to gradients in material stiffness. We observe that SA cells migrate up a stiffness gradient, or durotax, while WA cells largely ignore the gradient, i.e., adurotax. Biophysical modeling and experimental validation suggest that differences in cell migration and durotaxis between weakly and strongly adherent cells are driven by differences in intra-cellular actomyosin activity. These results provide a direct relationship between cell phenotype and durotaxis and suggest how, unlike other senescent cells, metastatic cancer cells navigate against stiffness gradients. 2021-03-09 /pmc/articles/PMC7997775/ /pubmed/33691109 http://dx.doi.org/10.1016/j.celrep.2021.108816 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Yeoman, Benjamin Shatkin, Gabriel Beri, Pranjali Banisadr, Afsheen Katira, Parag Engler, Adam J. Adhesion strength and contractility enable metastatic cells to become adurotactic |
title | Adhesion strength and contractility enable metastatic cells to become adurotactic |
title_full | Adhesion strength and contractility enable metastatic cells to become adurotactic |
title_fullStr | Adhesion strength and contractility enable metastatic cells to become adurotactic |
title_full_unstemmed | Adhesion strength and contractility enable metastatic cells to become adurotactic |
title_short | Adhesion strength and contractility enable metastatic cells to become adurotactic |
title_sort | adhesion strength and contractility enable metastatic cells to become adurotactic |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997775/ https://www.ncbi.nlm.nih.gov/pubmed/33691109 http://dx.doi.org/10.1016/j.celrep.2021.108816 |
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