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Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks

The rheological and physiological properties of red blood cells (RBCs) are affected by many factors in the vascular environment. Among them, membrane fluctuations (MFs), particularly dynamic fluctuations in RBC cell membrane thickness (RBC-MFs), are likely to be altered by the level of glycation of...

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Autores principales: Sohn, Minji, Lee, Ji Eun, Ahn, MinGeun, Park, YongKeun, Lim, Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997877/
https://www.ncbi.nlm.nih.gov/pubmed/33772071
http://dx.doi.org/10.1038/s41598-021-86528-0
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author Sohn, Minji
Lee, Ji Eun
Ahn, MinGeun
Park, YongKeun
Lim, Soo
author_facet Sohn, Minji
Lee, Ji Eun
Ahn, MinGeun
Park, YongKeun
Lim, Soo
author_sort Sohn, Minji
collection PubMed
description The rheological and physiological properties of red blood cells (RBCs) are affected by many factors in the vascular environment. Among them, membrane fluctuations (MFs), particularly dynamic fluctuations in RBC cell membrane thickness (RBC-MFs), are likely to be altered by the level of glycation of haemoglobin in patients with diabetes mellitus (DM). We investigated the associations of RBC-MFs with physiological variables associated with DM and cardiovascular diseases (CVDs). Forty-one healthy control subjects and 59 patients with DM were enrolled. Five-microliter samples of blood were collected and diluted 400 times. To measure the RBC-MFs, holotomography was used, which non-invasively and precisely analyses the optical characteristics of RBCs. Associations between the RBC-MFs and biochemical parameters related to glucose homeostasis and lipid profiles were investigated. Independent associations of the RBC-MFs with the presence of CVDs were also analysed. RBC-MFs were lower in patients with DM than in healthy participants (61.64 ± 7.49 nm vs 70.65 ± 6.65 nm, P = 1.4 × 10(−8)). RBC-MFs correlated modestly with glycated haemoglobin level (ρ = − 0.47) and weakly with age (ρ = − 0.36), duration of diabetes (ρ = − 0.36), fasting plasma glucose level (ρ = − 0.37), and the 10-year Framingham risk score (ρ = − 0.38) (all P < 0.05). Low RBC-MFs were independently associated with the presence of CVDs after adjusting for CVD risk factors. The weak but significant associations of RBC-MFs with cardiometabolic risk factors and CVDs suggest that such deformity of circulating RBCs may be a useful marker of vascular complications of DM.
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spelling pubmed-79978772021-03-29 Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks Sohn, Minji Lee, Ji Eun Ahn, MinGeun Park, YongKeun Lim, Soo Sci Rep Article The rheological and physiological properties of red blood cells (RBCs) are affected by many factors in the vascular environment. Among them, membrane fluctuations (MFs), particularly dynamic fluctuations in RBC cell membrane thickness (RBC-MFs), are likely to be altered by the level of glycation of haemoglobin in patients with diabetes mellitus (DM). We investigated the associations of RBC-MFs with physiological variables associated with DM and cardiovascular diseases (CVDs). Forty-one healthy control subjects and 59 patients with DM were enrolled. Five-microliter samples of blood were collected and diluted 400 times. To measure the RBC-MFs, holotomography was used, which non-invasively and precisely analyses the optical characteristics of RBCs. Associations between the RBC-MFs and biochemical parameters related to glucose homeostasis and lipid profiles were investigated. Independent associations of the RBC-MFs with the presence of CVDs were also analysed. RBC-MFs were lower in patients with DM than in healthy participants (61.64 ± 7.49 nm vs 70.65 ± 6.65 nm, P = 1.4 × 10(−8)). RBC-MFs correlated modestly with glycated haemoglobin level (ρ = − 0.47) and weakly with age (ρ = − 0.36), duration of diabetes (ρ = − 0.36), fasting plasma glucose level (ρ = − 0.37), and the 10-year Framingham risk score (ρ = − 0.38) (all P < 0.05). Low RBC-MFs were independently associated with the presence of CVDs after adjusting for CVD risk factors. The weak but significant associations of RBC-MFs with cardiometabolic risk factors and CVDs suggest that such deformity of circulating RBCs may be a useful marker of vascular complications of DM. Nature Publishing Group UK 2021-03-26 /pmc/articles/PMC7997877/ /pubmed/33772071 http://dx.doi.org/10.1038/s41598-021-86528-0 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sohn, Minji
Lee, Ji Eun
Ahn, MinGeun
Park, YongKeun
Lim, Soo
Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
title Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
title_full Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
title_fullStr Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
title_full_unstemmed Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
title_short Correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
title_sort correlation of dynamic membrane fluctuations in red blood cells with diabetes mellitus and cardiovascular risks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997877/
https://www.ncbi.nlm.nih.gov/pubmed/33772071
http://dx.doi.org/10.1038/s41598-021-86528-0
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