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An Insight into the Stages of Ion Leakage during Red Blood Cell Storage
Packed red blood cells (pRBCs), the most commonly transfused blood product, are exposed to environmental disruptions during storage in blood banks. In this study, temporal sequence of changes in the ion exchange in pRBCs was analyzed. Standard techniques commonly used in electrolyte measurements wer...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998123/ https://www.ncbi.nlm.nih.gov/pubmed/33809183 http://dx.doi.org/10.3390/ijms22062885 |
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author | Zimna, Anna Kaczmarska, Magdalena Szczesny-Malysiak, Ewa Wajda, Aleksandra Bulat, Katarzyna Alcicek, Fatih Celal Zygmunt, Malgorzata Sacha, Tomasz Marzec, Katarzyna Maria |
author_facet | Zimna, Anna Kaczmarska, Magdalena Szczesny-Malysiak, Ewa Wajda, Aleksandra Bulat, Katarzyna Alcicek, Fatih Celal Zygmunt, Malgorzata Sacha, Tomasz Marzec, Katarzyna Maria |
author_sort | Zimna, Anna |
collection | PubMed |
description | Packed red blood cells (pRBCs), the most commonly transfused blood product, are exposed to environmental disruptions during storage in blood banks. In this study, temporal sequence of changes in the ion exchange in pRBCs was analyzed. Standard techniques commonly used in electrolyte measurements were implemented. The relationship between ion exchange and red blood cells (RBCs) morphology was assessed with use of atomic force microscopy with reference to morphological parameters. Variations observed in the Na(+), K(+), Cl(−), H(+), HCO(3)(−), and lactate ions concentration show a complete picture of singly-charged ion changes in pRBCs during storage. Correlation between the rate of ion changes and blood group type, regarding the limitations of our research, suggested, that group 0 is the most sensitive to the time-dependent ionic changes. Additionally, the impact of irreversible changes in ion exchange on the RBCs membrane was observed in nanoscale. Results demonstrate that the level of ion leakage that leads to destructive alterations in biochemical and morphological properties of pRBCs depend on the storage timepoint. |
format | Online Article Text |
id | pubmed-7998123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79981232021-03-28 An Insight into the Stages of Ion Leakage during Red Blood Cell Storage Zimna, Anna Kaczmarska, Magdalena Szczesny-Malysiak, Ewa Wajda, Aleksandra Bulat, Katarzyna Alcicek, Fatih Celal Zygmunt, Malgorzata Sacha, Tomasz Marzec, Katarzyna Maria Int J Mol Sci Article Packed red blood cells (pRBCs), the most commonly transfused blood product, are exposed to environmental disruptions during storage in blood banks. In this study, temporal sequence of changes in the ion exchange in pRBCs was analyzed. Standard techniques commonly used in electrolyte measurements were implemented. The relationship between ion exchange and red blood cells (RBCs) morphology was assessed with use of atomic force microscopy with reference to morphological parameters. Variations observed in the Na(+), K(+), Cl(−), H(+), HCO(3)(−), and lactate ions concentration show a complete picture of singly-charged ion changes in pRBCs during storage. Correlation between the rate of ion changes and blood group type, regarding the limitations of our research, suggested, that group 0 is the most sensitive to the time-dependent ionic changes. Additionally, the impact of irreversible changes in ion exchange on the RBCs membrane was observed in nanoscale. Results demonstrate that the level of ion leakage that leads to destructive alterations in biochemical and morphological properties of pRBCs depend on the storage timepoint. MDPI 2021-03-12 /pmc/articles/PMC7998123/ /pubmed/33809183 http://dx.doi.org/10.3390/ijms22062885 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zimna, Anna Kaczmarska, Magdalena Szczesny-Malysiak, Ewa Wajda, Aleksandra Bulat, Katarzyna Alcicek, Fatih Celal Zygmunt, Malgorzata Sacha, Tomasz Marzec, Katarzyna Maria An Insight into the Stages of Ion Leakage during Red Blood Cell Storage |
title | An Insight into the Stages of Ion Leakage during Red Blood Cell Storage |
title_full | An Insight into the Stages of Ion Leakage during Red Blood Cell Storage |
title_fullStr | An Insight into the Stages of Ion Leakage during Red Blood Cell Storage |
title_full_unstemmed | An Insight into the Stages of Ion Leakage during Red Blood Cell Storage |
title_short | An Insight into the Stages of Ion Leakage during Red Blood Cell Storage |
title_sort | insight into the stages of ion leakage during red blood cell storage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998123/ https://www.ncbi.nlm.nih.gov/pubmed/33809183 http://dx.doi.org/10.3390/ijms22062885 |
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