Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2

We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 ki...

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Detalles Bibliográficos
Autores principales: Curti, Lucía Ana, Primost, Ivana, Valla, Sofia, Ibañez Alegre, Daiana, Olguin Perglione, Cecilia, Repizo, Guillermo Daniel, Lara, Julia, Parcerisa, Ivana, Palacios, Antonela, Llases, María Eugenia, Rinflerch, Adriana, Barrios, Melanie, Pereyra Bonnet, Federico, Gimenez, Carla Alejandra, Marcone, Débora Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998296/
https://www.ncbi.nlm.nih.gov/pubmed/33807908
http://dx.doi.org/10.3390/v13030420

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