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Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)

F-box genes play an important role in the growth and development of plants, but there are few studies on its role in a plant’s response to abiotic stresses. In order to further study the functions of F-box genes in tomato (Solanum lycopersicum, Sl), a total of 139 F-box genes were identified in the...

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Autores principales: Mo, Fulei, Zhang, Nian, Qiu, Youwen, Meng, Lingjun, Cheng, Mozhen, Liu, Jiayin, Yao, Lanning, Lv, Rui, Liu, Yuxin, Zhang, Yao, Chen, Xiuling, Wang, Aoxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998346/
https://www.ncbi.nlm.nih.gov/pubmed/33799396
http://dx.doi.org/10.3390/genes12030417
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author Mo, Fulei
Zhang, Nian
Qiu, Youwen
Meng, Lingjun
Cheng, Mozhen
Liu, Jiayin
Yao, Lanning
Lv, Rui
Liu, Yuxin
Zhang, Yao
Chen, Xiuling
Wang, Aoxue
author_facet Mo, Fulei
Zhang, Nian
Qiu, Youwen
Meng, Lingjun
Cheng, Mozhen
Liu, Jiayin
Yao, Lanning
Lv, Rui
Liu, Yuxin
Zhang, Yao
Chen, Xiuling
Wang, Aoxue
author_sort Mo, Fulei
collection PubMed
description F-box genes play an important role in the growth and development of plants, but there are few studies on its role in a plant’s response to abiotic stresses. In order to further study the functions of F-box genes in tomato (Solanum lycopersicum, Sl), a total of 139 F-box genes were identified in the whole genome of tomato using bioinformatics methods, and the basic information, transcript structure, conserved motif, cis-elements, chromosomal location, gene evolution, phylogenetic relationship, expression patterns and the expression under cold stress, drought stress, jasmonic acid (JA) treatment and salicylic acid (SA) treatment were analyzed. The results showed that SlFBX genes were distributed on 12 chromosomes of tomato and were prone to TD (tandem duplication) at the ends of chromosomes. WGD (whole genome duplication), TD, PD (proximal duplication) and TRD (transposed duplication) modes seem play an important role in the expansion and evolution of tomato SlFBX genes. The most recent divergence occurred 1.3042 million years ago, between SlFBX89 and SlFBX103. The cis-elements in SlFBX genes’ promoter regions were mainly responded to phytohormone and abiotic stress. Expression analysis based on transcriptome data and qRT-PCR (Real-time quantitative PCR) analysis of SlFBX genes showed that most SlFBX genes were differentially expressed under abiotic stress. SlFBX24 was significantly up-regulated at 12 h under cold stress. This study reported the SlFBX gene family of tomato for the first time, providing a theoretical basis for the detailed study of SlFBX genes in the future, especially the function of SlFBX genes under abiotic stress.
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spelling pubmed-79983462021-03-28 Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum) Mo, Fulei Zhang, Nian Qiu, Youwen Meng, Lingjun Cheng, Mozhen Liu, Jiayin Yao, Lanning Lv, Rui Liu, Yuxin Zhang, Yao Chen, Xiuling Wang, Aoxue Genes (Basel) Article F-box genes play an important role in the growth and development of plants, but there are few studies on its role in a plant’s response to abiotic stresses. In order to further study the functions of F-box genes in tomato (Solanum lycopersicum, Sl), a total of 139 F-box genes were identified in the whole genome of tomato using bioinformatics methods, and the basic information, transcript structure, conserved motif, cis-elements, chromosomal location, gene evolution, phylogenetic relationship, expression patterns and the expression under cold stress, drought stress, jasmonic acid (JA) treatment and salicylic acid (SA) treatment were analyzed. The results showed that SlFBX genes were distributed on 12 chromosomes of tomato and were prone to TD (tandem duplication) at the ends of chromosomes. WGD (whole genome duplication), TD, PD (proximal duplication) and TRD (transposed duplication) modes seem play an important role in the expansion and evolution of tomato SlFBX genes. The most recent divergence occurred 1.3042 million years ago, between SlFBX89 and SlFBX103. The cis-elements in SlFBX genes’ promoter regions were mainly responded to phytohormone and abiotic stress. Expression analysis based on transcriptome data and qRT-PCR (Real-time quantitative PCR) analysis of SlFBX genes showed that most SlFBX genes were differentially expressed under abiotic stress. SlFBX24 was significantly up-regulated at 12 h under cold stress. This study reported the SlFBX gene family of tomato for the first time, providing a theoretical basis for the detailed study of SlFBX genes in the future, especially the function of SlFBX genes under abiotic stress. MDPI 2021-03-14 /pmc/articles/PMC7998346/ /pubmed/33799396 http://dx.doi.org/10.3390/genes12030417 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Mo, Fulei
Zhang, Nian
Qiu, Youwen
Meng, Lingjun
Cheng, Mozhen
Liu, Jiayin
Yao, Lanning
Lv, Rui
Liu, Yuxin
Zhang, Yao
Chen, Xiuling
Wang, Aoxue
Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
title Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
title_full Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
title_fullStr Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
title_full_unstemmed Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
title_short Molecular Characterization, Gene Evolution and Expression Analysis of the F-Box Gene Family in Tomato (Solanum lycopersicum)
title_sort molecular characterization, gene evolution and expression analysis of the f-box gene family in tomato (solanum lycopersicum)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998346/
https://www.ncbi.nlm.nih.gov/pubmed/33799396
http://dx.doi.org/10.3390/genes12030417
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