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Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome

Idiopathic nephrotic syndrome (INS) is the most frequent primary glomerular disease in children, displaying high grade proteinuria and oedema. The mainstay of therapy are steroids, and patients are usually classified according to the treatment response (sensitive vs. resistant). The mechanisms invol...

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Autores principales: Santorelli, Lucia, Morello, William, Barigazzi, Elisa, Capitoli, Giulia, Tamburello, Chiara, Ghio, Luciana, Crapella, Barbara, Galimberti, Stefania, Montini, Giovanni, Pitto, Marina, Raimondo, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998527/
https://www.ncbi.nlm.nih.gov/pubmed/33800879
http://dx.doi.org/10.3390/diagnostics11030456
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author Santorelli, Lucia
Morello, William
Barigazzi, Elisa
Capitoli, Giulia
Tamburello, Chiara
Ghio, Luciana
Crapella, Barbara
Galimberti, Stefania
Montini, Giovanni
Pitto, Marina
Raimondo, Francesca
author_facet Santorelli, Lucia
Morello, William
Barigazzi, Elisa
Capitoli, Giulia
Tamburello, Chiara
Ghio, Luciana
Crapella, Barbara
Galimberti, Stefania
Montini, Giovanni
Pitto, Marina
Raimondo, Francesca
author_sort Santorelli, Lucia
collection PubMed
description Idiopathic nephrotic syndrome (INS) is the most frequent primary glomerular disease in children, displaying high grade proteinuria and oedema. The mainstay of therapy are steroids, and patients are usually classified according to the treatment response (sensitive vs. resistant). The mechanisms involved in INS pathogenesis and treatment responsiveness have not yet been identified. In this context, the analysis of urinary extracellular vesicles (UEv) is interesting, since they represent a molecular snapshot of the parental cells, offering a “fingerprint” for monitoring their status. Therefore, the aim of this study is to verify the feasibility of using UEv of INS patients as indicators of therapy response and its prediction. UEv were isolated from the urine of pediatric patients in remission after therapy; they showed characteristic electrophoresis profiles that matched specific patient subgroups. We then built a statistical model to interpret objectively each patient UEv protein profile: in particular, steroid-resistant patients cluster together with a very distinct pattern from other INS patients and controls. In conclusion, the evaluation of the UEv protein profile looks promising in the investigation of INS, showing a disease signature that might predict clinical evolution.
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spelling pubmed-79985272021-03-28 Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome Santorelli, Lucia Morello, William Barigazzi, Elisa Capitoli, Giulia Tamburello, Chiara Ghio, Luciana Crapella, Barbara Galimberti, Stefania Montini, Giovanni Pitto, Marina Raimondo, Francesca Diagnostics (Basel) Article Idiopathic nephrotic syndrome (INS) is the most frequent primary glomerular disease in children, displaying high grade proteinuria and oedema. The mainstay of therapy are steroids, and patients are usually classified according to the treatment response (sensitive vs. resistant). The mechanisms involved in INS pathogenesis and treatment responsiveness have not yet been identified. In this context, the analysis of urinary extracellular vesicles (UEv) is interesting, since they represent a molecular snapshot of the parental cells, offering a “fingerprint” for monitoring their status. Therefore, the aim of this study is to verify the feasibility of using UEv of INS patients as indicators of therapy response and its prediction. UEv were isolated from the urine of pediatric patients in remission after therapy; they showed characteristic electrophoresis profiles that matched specific patient subgroups. We then built a statistical model to interpret objectively each patient UEv protein profile: in particular, steroid-resistant patients cluster together with a very distinct pattern from other INS patients and controls. In conclusion, the evaluation of the UEv protein profile looks promising in the investigation of INS, showing a disease signature that might predict clinical evolution. MDPI 2021-03-06 /pmc/articles/PMC7998527/ /pubmed/33800879 http://dx.doi.org/10.3390/diagnostics11030456 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Santorelli, Lucia
Morello, William
Barigazzi, Elisa
Capitoli, Giulia
Tamburello, Chiara
Ghio, Luciana
Crapella, Barbara
Galimberti, Stefania
Montini, Giovanni
Pitto, Marina
Raimondo, Francesca
Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome
title Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome
title_full Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome
title_fullStr Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome
title_full_unstemmed Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome
title_short Urinary Extracellular Vesicle Protein Profiles Discriminate Different Clinical Subgroups of Children with Idiopathic Nephrotic Syndrome
title_sort urinary extracellular vesicle protein profiles discriminate different clinical subgroups of children with idiopathic nephrotic syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998527/
https://www.ncbi.nlm.nih.gov/pubmed/33800879
http://dx.doi.org/10.3390/diagnostics11030456
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