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Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter...

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Autores principales: Sciortino, Sonia, Arculeo, Pietro, Alio, Vincenzina, Cardamone, Cinzia, Nicastro, Luisa, Arculeo, Marco, Alduina, Rosa, Costa, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998538/
https://www.ncbi.nlm.nih.gov/pubmed/33802125
http://dx.doi.org/10.3390/antibiotics10030288
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author Sciortino, Sonia
Arculeo, Pietro
Alio, Vincenzina
Cardamone, Cinzia
Nicastro, Luisa
Arculeo, Marco
Alduina, Rosa
Costa, Antonella
author_facet Sciortino, Sonia
Arculeo, Pietro
Alio, Vincenzina
Cardamone, Cinzia
Nicastro, Luisa
Arculeo, Marco
Alduina, Rosa
Costa, Antonella
author_sort Sciortino, Sonia
collection PubMed
description Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of tetO and/or tetW. This work confirms the occurrence and the continuous emergence of antibiotic-resistant Arcobacter strains in environmental samples; also, the presence of quinolone-resistant Arcobacter spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health.
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spelling pubmed-79985382021-03-28 Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments Sciortino, Sonia Arculeo, Pietro Alio, Vincenzina Cardamone, Cinzia Nicastro, Luisa Arculeo, Marco Alduina, Rosa Costa, Antonella Antibiotics (Basel) Article Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of tetO and/or tetW. This work confirms the occurrence and the continuous emergence of antibiotic-resistant Arcobacter strains in environmental samples; also, the presence of quinolone-resistant Arcobacter spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health. MDPI 2021-03-10 /pmc/articles/PMC7998538/ /pubmed/33802125 http://dx.doi.org/10.3390/antibiotics10030288 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Sciortino, Sonia
Arculeo, Pietro
Alio, Vincenzina
Cardamone, Cinzia
Nicastro, Luisa
Arculeo, Marco
Alduina, Rosa
Costa, Antonella
Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
title Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
title_full Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
title_fullStr Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
title_full_unstemmed Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
title_short Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments
title_sort occurrence and antimicrobial resistance of arcobacter spp. recovered from aquatic environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998538/
https://www.ncbi.nlm.nih.gov/pubmed/33802125
http://dx.doi.org/10.3390/antibiotics10030288
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