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Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells
T cell activation mediates immunity to pathogens. On the flipside, T cells are also involved in pathological immune responses during chronic autoimmune diseases. We recently reported that zinc aspartate, a registered drug with high bioavailability, dose-dependently inhibits T cell activation and Th1...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998552/ https://www.ncbi.nlm.nih.gov/pubmed/33804583 http://dx.doi.org/10.3390/biomedicines9030246 |
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author | Reinhold, Dirk Guttek, Karina Reddig, Annika Voss, Linda Schubert, Claudia Kahlfuss, Sascha Grüngreiff, Kurt Schraven, Burkhart Reinhold, Annegret |
author_facet | Reinhold, Dirk Guttek, Karina Reddig, Annika Voss, Linda Schubert, Claudia Kahlfuss, Sascha Grüngreiff, Kurt Schraven, Burkhart Reinhold, Annegret |
author_sort | Reinhold, Dirk |
collection | PubMed |
description | T cell activation mediates immunity to pathogens. On the flipside, T cells are also involved in pathological immune responses during chronic autoimmune diseases. We recently reported that zinc aspartate, a registered drug with high bioavailability, dose-dependently inhibits T cell activation and Th1/Th2/Th17 cytokine production of stimulated human and mouse T cells. To understand the suppressive effect of zinc on T cell function, we here investigated the influence of zinc aspartate on human T cells focusing on the secretion of immunosuppressive cytokines, induction of apoptosis, and caspase 3/7 activity. To this end, we monitored either freshly stimulated or pre-activated human T cells in the presence of zinc aspartate from 40–140 µM over a period of 72 h. Under both experimental conditions, we observed a dose-dependent suppression of human T cell proliferation. While IL-1ra, latent TGF-β1, and IL-10 were dose-dependently reduced, we, unexpectedly, detected elevated levels of IL-16 upon zinc supplementation. In addition, the number of cells with active caspase 3/7 and, consecutively, the amount of cells undergoing apoptosis, steadily increased at zinc aspartate concentrations exceeding 100 µM. Taken together, our findings suggest that zinc aspartate impairs T cell fitness and might be beneficial for the treatment of T cell-mediated autoimmune diseases. |
format | Online Article Text |
id | pubmed-7998552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79985522021-03-28 Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells Reinhold, Dirk Guttek, Karina Reddig, Annika Voss, Linda Schubert, Claudia Kahlfuss, Sascha Grüngreiff, Kurt Schraven, Burkhart Reinhold, Annegret Biomedicines Article T cell activation mediates immunity to pathogens. On the flipside, T cells are also involved in pathological immune responses during chronic autoimmune diseases. We recently reported that zinc aspartate, a registered drug with high bioavailability, dose-dependently inhibits T cell activation and Th1/Th2/Th17 cytokine production of stimulated human and mouse T cells. To understand the suppressive effect of zinc on T cell function, we here investigated the influence of zinc aspartate on human T cells focusing on the secretion of immunosuppressive cytokines, induction of apoptosis, and caspase 3/7 activity. To this end, we monitored either freshly stimulated or pre-activated human T cells in the presence of zinc aspartate from 40–140 µM over a period of 72 h. Under both experimental conditions, we observed a dose-dependent suppression of human T cell proliferation. While IL-1ra, latent TGF-β1, and IL-10 were dose-dependently reduced, we, unexpectedly, detected elevated levels of IL-16 upon zinc supplementation. In addition, the number of cells with active caspase 3/7 and, consecutively, the amount of cells undergoing apoptosis, steadily increased at zinc aspartate concentrations exceeding 100 µM. Taken together, our findings suggest that zinc aspartate impairs T cell fitness and might be beneficial for the treatment of T cell-mediated autoimmune diseases. MDPI 2021-03-01 /pmc/articles/PMC7998552/ /pubmed/33804583 http://dx.doi.org/10.3390/biomedicines9030246 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Reinhold, Dirk Guttek, Karina Reddig, Annika Voss, Linda Schubert, Claudia Kahlfuss, Sascha Grüngreiff, Kurt Schraven, Burkhart Reinhold, Annegret Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells |
title | Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells |
title_full | Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells |
title_fullStr | Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells |
title_full_unstemmed | Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells |
title_short | Zinc Aspartate Induces IL-16 Secretion and Apoptosis in Human T Cells |
title_sort | zinc aspartate induces il-16 secretion and apoptosis in human t cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998552/ https://www.ncbi.nlm.nih.gov/pubmed/33804583 http://dx.doi.org/10.3390/biomedicines9030246 |
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