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Molecular Evolution of Infectious Pancreatic Necrosis Virus in China
Passive virus surveillance was performed in twenty-nine salmon and trout farms from seven provinces and districts in China during the period 2017–2020. A total of 25 infectious pancreatic necrosis virus (IPNV) isolates were obtained, mainly from rainbow trout (Oncorhynchus mykiss). The molecular evo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998647/ https://www.ncbi.nlm.nih.gov/pubmed/33809489 http://dx.doi.org/10.3390/v13030488 |
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author | Duan, Kaiyue Zhao, Jingzhuang Ren, Guangming Shao, Yizhi Lu, Tongyan Xu, Lipu Tang, Xin Zhao, Wenwen Xu, Liming |
author_facet | Duan, Kaiyue Zhao, Jingzhuang Ren, Guangming Shao, Yizhi Lu, Tongyan Xu, Lipu Tang, Xin Zhao, Wenwen Xu, Liming |
author_sort | Duan, Kaiyue |
collection | PubMed |
description | Passive virus surveillance was performed in twenty-nine salmon and trout farms from seven provinces and districts in China during the period 2017–2020. A total of 25 infectious pancreatic necrosis virus (IPNV) isolates were obtained, mainly from rainbow trout (Oncorhynchus mykiss). The molecular evolution of these Chinese IPNV isolates and the previously reported Chinese IPNV strains ChRtm213 and WZ2016 was analyzed, based on their VP2 gene coding region sequences (CDS). All 27 Chinese IPNV isolates clustered within genogroups I and V, with 24 of the IPNV isolates belonging to genogroup I (including ChRtm213 and WZ2016), and only three isolates clustering in genogroup V. The Chinese genogroup I IPNV isolates lacked diversity, composing six haplotypes with 41 polymorphic sites, and the identity of nucleotide and amino acid sequences among the entire VP2 gene CDS from these isolates was 97.44%–100% and 98.19%–100%, respectively. Divergence time analyses revealed that the Chinese genogroup I IPNV isolates likely diverged from Japanese IPNV isolates in 1985 (95% highest posterior density (HPD), 1965–1997), and diverged again in 2006 (95% HPD, 1996–2013) in China. Each of the three Chinese genogroup V IPNV isolates has a unique VP2 gene CDS, with a total of 21 polymorphic sites; the identity of nucleotide and amino acid sequences among all VP2 gene CDS from these isolates was 98.5%–99.5% and 98.6%–99.0%, respectively. The data demonstrate that genogroups I and V are more likely the currently prevalent Chinese IPNV genotypes. |
format | Online Article Text |
id | pubmed-7998647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79986472021-03-28 Molecular Evolution of Infectious Pancreatic Necrosis Virus in China Duan, Kaiyue Zhao, Jingzhuang Ren, Guangming Shao, Yizhi Lu, Tongyan Xu, Lipu Tang, Xin Zhao, Wenwen Xu, Liming Viruses Article Passive virus surveillance was performed in twenty-nine salmon and trout farms from seven provinces and districts in China during the period 2017–2020. A total of 25 infectious pancreatic necrosis virus (IPNV) isolates were obtained, mainly from rainbow trout (Oncorhynchus mykiss). The molecular evolution of these Chinese IPNV isolates and the previously reported Chinese IPNV strains ChRtm213 and WZ2016 was analyzed, based on their VP2 gene coding region sequences (CDS). All 27 Chinese IPNV isolates clustered within genogroups I and V, with 24 of the IPNV isolates belonging to genogroup I (including ChRtm213 and WZ2016), and only three isolates clustering in genogroup V. The Chinese genogroup I IPNV isolates lacked diversity, composing six haplotypes with 41 polymorphic sites, and the identity of nucleotide and amino acid sequences among the entire VP2 gene CDS from these isolates was 97.44%–100% and 98.19%–100%, respectively. Divergence time analyses revealed that the Chinese genogroup I IPNV isolates likely diverged from Japanese IPNV isolates in 1985 (95% highest posterior density (HPD), 1965–1997), and diverged again in 2006 (95% HPD, 1996–2013) in China. Each of the three Chinese genogroup V IPNV isolates has a unique VP2 gene CDS, with a total of 21 polymorphic sites; the identity of nucleotide and amino acid sequences among all VP2 gene CDS from these isolates was 98.5%–99.5% and 98.6%–99.0%, respectively. The data demonstrate that genogroups I and V are more likely the currently prevalent Chinese IPNV genotypes. MDPI 2021-03-16 /pmc/articles/PMC7998647/ /pubmed/33809489 http://dx.doi.org/10.3390/v13030488 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Duan, Kaiyue Zhao, Jingzhuang Ren, Guangming Shao, Yizhi Lu, Tongyan Xu, Lipu Tang, Xin Zhao, Wenwen Xu, Liming Molecular Evolution of Infectious Pancreatic Necrosis Virus in China |
title | Molecular Evolution of Infectious Pancreatic Necrosis Virus in China |
title_full | Molecular Evolution of Infectious Pancreatic Necrosis Virus in China |
title_fullStr | Molecular Evolution of Infectious Pancreatic Necrosis Virus in China |
title_full_unstemmed | Molecular Evolution of Infectious Pancreatic Necrosis Virus in China |
title_short | Molecular Evolution of Infectious Pancreatic Necrosis Virus in China |
title_sort | molecular evolution of infectious pancreatic necrosis virus in china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998647/ https://www.ncbi.nlm.nih.gov/pubmed/33809489 http://dx.doi.org/10.3390/v13030488 |
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