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Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans

Methicillin-resistant Staphylococcus aureus (MRSA) and non-aureus staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette mec (SCCmec) types of MRSA or MRNAS isolated fro...

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Autores principales: Ngassam Tchamba, Cyrille, Duprez, Jean-Noël, Lucas, Pierrick, Blanchard, Yannick, Boyen, Filip, Haesebrouck, Freddy, Argudín, Maria A., Mainil, Jacques, Thiry, Damien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998684/
https://www.ncbi.nlm.nih.gov/pubmed/33806351
http://dx.doi.org/10.3390/antibiotics10030256
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author Ngassam Tchamba, Cyrille
Duprez, Jean-Noël
Lucas, Pierrick
Blanchard, Yannick
Boyen, Filip
Haesebrouck, Freddy
Argudín, Maria A.
Mainil, Jacques
Thiry, Damien
author_facet Ngassam Tchamba, Cyrille
Duprez, Jean-Noël
Lucas, Pierrick
Blanchard, Yannick
Boyen, Filip
Haesebrouck, Freddy
Argudín, Maria A.
Mainil, Jacques
Thiry, Damien
author_sort Ngassam Tchamba, Cyrille
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) and non-aureus staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette mec (SCCmec) types of MRSA or MRNAS isolated from several animal species and humans in different countries. Of 1462 S. aureus and non-aureus staphylococci, 68 grew on Chrom MRSA ID(®) agar, were phenotypically resistant to cefoxitin and tested positive with the PCR for the mecA gene. These 60 MRSA and 8 MRNAS were isolated in Belgium mainly from cows (livestock-associated (LA) MRS) and humans (community-acquired (CA) MRS) and in Japan from dogs and cats. The SCCmec cassettes were identified by multiplex PCR in 52 MRSA and 7 MRNAS and by whole genome sequencing (WGS) in 8 additional MRSA. The SCCmec types IV and V were the most frequent in Belgian LA-MRS and CA-MRS, while the SCCmec type II was identified in four of the five Japanese MRSA. The remaining isolate was a bovine S. haemolyticus in which no SCCmec was identified. These results confirm the high prevalence of the SCCmec types IV and V in LA-MRS and CA-MRS in Belgium, emphasizing the possible public health hazard of the former, and the absence of SCCmec in some MRNAS.
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spelling pubmed-79986842021-03-28 Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans Ngassam Tchamba, Cyrille Duprez, Jean-Noël Lucas, Pierrick Blanchard, Yannick Boyen, Filip Haesebrouck, Freddy Argudín, Maria A. Mainil, Jacques Thiry, Damien Antibiotics (Basel) Article Methicillin-resistant Staphylococcus aureus (MRSA) and non-aureus staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette mec (SCCmec) types of MRSA or MRNAS isolated from several animal species and humans in different countries. Of 1462 S. aureus and non-aureus staphylococci, 68 grew on Chrom MRSA ID(®) agar, were phenotypically resistant to cefoxitin and tested positive with the PCR for the mecA gene. These 60 MRSA and 8 MRNAS were isolated in Belgium mainly from cows (livestock-associated (LA) MRS) and humans (community-acquired (CA) MRS) and in Japan from dogs and cats. The SCCmec cassettes were identified by multiplex PCR in 52 MRSA and 7 MRNAS and by whole genome sequencing (WGS) in 8 additional MRSA. The SCCmec types IV and V were the most frequent in Belgian LA-MRS and CA-MRS, while the SCCmec type II was identified in four of the five Japanese MRSA. The remaining isolate was a bovine S. haemolyticus in which no SCCmec was identified. These results confirm the high prevalence of the SCCmec types IV and V in LA-MRS and CA-MRS in Belgium, emphasizing the possible public health hazard of the former, and the absence of SCCmec in some MRNAS. MDPI 2021-03-04 /pmc/articles/PMC7998684/ /pubmed/33806351 http://dx.doi.org/10.3390/antibiotics10030256 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Ngassam Tchamba, Cyrille
Duprez, Jean-Noël
Lucas, Pierrick
Blanchard, Yannick
Boyen, Filip
Haesebrouck, Freddy
Argudín, Maria A.
Mainil, Jacques
Thiry, Damien
Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans
title Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans
title_full Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans
title_fullStr Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans
title_full_unstemmed Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans
title_short Comparison of the Staphylococcal Chromosome Cassette (SCC) mec in Methicillin-Resistant Staphylococcus aureus (MRSA) and Non-aureus Staphylococci (MRNAS) from Animals and Humans
title_sort comparison of the staphylococcal chromosome cassette (scc) mec in methicillin-resistant staphylococcus aureus (mrsa) and non-aureus staphylococci (mrnas) from animals and humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998684/
https://www.ncbi.nlm.nih.gov/pubmed/33806351
http://dx.doi.org/10.3390/antibiotics10030256
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