Cargando…

Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection

The mitochondrial antiviral-signaling protein (MAVS, also known as VISA, IPS-1, or CARDIF) plays an essential role in the type I interferon (IFN) response and in retinoic acid-inducible gene I (RIG-I) mediated antiviral innate immunity in mammals. In this study, the caprine MAVS gene (caMAVS, 1566 b...

Descripción completa

Detalles Bibliográficos
Autores principales: Miao, Qiuhong, Qi, Ruibing, Meng, Chunchun, Zhu, Jie, Tang, Aoxing, Dong, Dandan, Guo, Hongyuan, van Oers, Monique M., Pijlman, Gorben P., Liu, Guangqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998690/
https://www.ncbi.nlm.nih.gov/pubmed/33807534
http://dx.doi.org/10.3390/v13030409
_version_ 1783670610186993664
author Miao, Qiuhong
Qi, Ruibing
Meng, Chunchun
Zhu, Jie
Tang, Aoxing
Dong, Dandan
Guo, Hongyuan
van Oers, Monique M.
Pijlman, Gorben P.
Liu, Guangqing
author_facet Miao, Qiuhong
Qi, Ruibing
Meng, Chunchun
Zhu, Jie
Tang, Aoxing
Dong, Dandan
Guo, Hongyuan
van Oers, Monique M.
Pijlman, Gorben P.
Liu, Guangqing
author_sort Miao, Qiuhong
collection PubMed
description The mitochondrial antiviral-signaling protein (MAVS, also known as VISA, IPS-1, or CARDIF) plays an essential role in the type I interferon (IFN) response and in retinoic acid-inducible gene I (RIG-I) mediated antiviral innate immunity in mammals. In this study, the caprine MAVS gene (caMAVS, 1566 bp) was identified and cloned. The caMAVS shares the highest amino acid similarity (98.1%) with the predicted sheep MAVS. Confocal microscopy analysis of partial deletion mutants of caMAVS revealed that the transmembrane and the so-called Non-Characterized domains are indispensable for intracellular localization to mitochondria. Overexpression of caMAVS in caprine endometrial epithelial cells up-regulated the mRNA levels of caprine interferon-stimulated genes. We concluded that caprine MAVS mediates the activation of the type I IFN pathway. We further demonstrated that both the CARD-like domain and the transmembrane domain of caMAVS were essential for the activation of the IFN-β promotor. The interaction between caMAVS and caprine RIG-I and the vital role of the CARD and NC domain in this interaction was demonstrated by co-immunoprecipitation. Upon infection with the Peste des Petits Ruminants Virus (PPRV, genus Morbillivirus), the level of MAVS was greatly reduced. This reduction was prevented by the addition of the proteasome inhibitor MG132. Moreover, we found that viral protein V could interact and colocalize with MAVS. Together, we identified caMAVS as a RIG-I interactive protein involved in the activation of type I IFN pathways in caprine cells and as a target for PPRV immune evasion.
format Online
Article
Text
id pubmed-7998690
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79986902021-03-28 Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection Miao, Qiuhong Qi, Ruibing Meng, Chunchun Zhu, Jie Tang, Aoxing Dong, Dandan Guo, Hongyuan van Oers, Monique M. Pijlman, Gorben P. Liu, Guangqing Viruses Article The mitochondrial antiviral-signaling protein (MAVS, also known as VISA, IPS-1, or CARDIF) plays an essential role in the type I interferon (IFN) response and in retinoic acid-inducible gene I (RIG-I) mediated antiviral innate immunity in mammals. In this study, the caprine MAVS gene (caMAVS, 1566 bp) was identified and cloned. The caMAVS shares the highest amino acid similarity (98.1%) with the predicted sheep MAVS. Confocal microscopy analysis of partial deletion mutants of caMAVS revealed that the transmembrane and the so-called Non-Characterized domains are indispensable for intracellular localization to mitochondria. Overexpression of caMAVS in caprine endometrial epithelial cells up-regulated the mRNA levels of caprine interferon-stimulated genes. We concluded that caprine MAVS mediates the activation of the type I IFN pathway. We further demonstrated that both the CARD-like domain and the transmembrane domain of caMAVS were essential for the activation of the IFN-β promotor. The interaction between caMAVS and caprine RIG-I and the vital role of the CARD and NC domain in this interaction was demonstrated by co-immunoprecipitation. Upon infection with the Peste des Petits Ruminants Virus (PPRV, genus Morbillivirus), the level of MAVS was greatly reduced. This reduction was prevented by the addition of the proteasome inhibitor MG132. Moreover, we found that viral protein V could interact and colocalize with MAVS. Together, we identified caMAVS as a RIG-I interactive protein involved in the activation of type I IFN pathways in caprine cells and as a target for PPRV immune evasion. MDPI 2021-03-05 /pmc/articles/PMC7998690/ /pubmed/33807534 http://dx.doi.org/10.3390/v13030409 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Miao, Qiuhong
Qi, Ruibing
Meng, Chunchun
Zhu, Jie
Tang, Aoxing
Dong, Dandan
Guo, Hongyuan
van Oers, Monique M.
Pijlman, Gorben P.
Liu, Guangqing
Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
title Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
title_full Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
title_fullStr Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
title_full_unstemmed Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
title_short Caprine MAVS Is a RIG-I Interacting Type I Interferon Inducer Downregulated by Peste des Petits Ruminants Virus Infection
title_sort caprine mavs is a rig-i interacting type i interferon inducer downregulated by peste des petits ruminants virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998690/
https://www.ncbi.nlm.nih.gov/pubmed/33807534
http://dx.doi.org/10.3390/v13030409
work_keys_str_mv AT miaoqiuhong caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT qiruibing caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT mengchunchun caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT zhujie caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT tangaoxing caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT dongdandan caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT guohongyuan caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT vanoersmoniquem caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT pijlmangorbenp caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection
AT liuguangqing caprinemavsisarigiinteractingtypeiinterferoninducerdownregulatedbypestedespetitsruminantsvirusinfection