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Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles

Magnetic-plasmonic, Fe(3)O(4)-Au, core-shell nanoparticles are popular in many applications, most notably in therapeutics and diagnostics, and thus, the imaging of these nanostructures in biological samples is of high importance. These nanostructures are typically imaged in biological material by da...

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Autores principales: Brennan, Grace, Ryan, Sally, Soulimane, Tewfik, Tofail, Syed A. M., Silien, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998699/
https://www.ncbi.nlm.nih.gov/pubmed/33803430
http://dx.doi.org/10.3390/nano11030685
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author Brennan, Grace
Ryan, Sally
Soulimane, Tewfik
Tofail, Syed A. M.
Silien, Christophe
author_facet Brennan, Grace
Ryan, Sally
Soulimane, Tewfik
Tofail, Syed A. M.
Silien, Christophe
author_sort Brennan, Grace
collection PubMed
description Magnetic-plasmonic, Fe(3)O(4)-Au, core-shell nanoparticles are popular in many applications, most notably in therapeutics and diagnostics, and thus, the imaging of these nanostructures in biological samples is of high importance. These nanostructures are typically imaged in biological material by dark field scatter imaging, which requires an even distribution of nanostructures in the sample and, therefore, high nanoparticle doses, potentially leading to toxicology issues. Herein, we explore the nonlinear optical properties of magnetic nanoparticles coated with various thicknesses of gold using the open aperture z-scan technique to determine the nonlinear optical properties and moreover, predict the efficacy of the nanostructures in nonlinear imaging. We find that the magnetic nanoparticles coated with gold nanoseeds and thinner gold shells (ca. 4 nm) show the largest nonlinear absorption coefficient β and imaginary part of the third-order susceptibility Im χ((3)), suggesting that these nanostructures would be suitable contrast agents. Next, we combine laser dark field microscopy and epi-detected coherent anti-Stokes Raman (CARS) microscopy to image the uptake of magnetic-plasmonic nanoparticles in human pancreatic cancer cells. We show the epi-detected CARS technique is suitable for imaging of the magnetic-plasmonic nanoparticles without requiring a dense distribution of nanoparticles. This technique achieves superior nanoparticle contrasting over both epi-detected backscatter imaging and transmission dark field imaging, while also attaining label-free chemical contrasting of the cell. Lastly, we show the high biocompatibility of the Fe(3)O(4) nanoparticles with ca. 4-nm thick Au shell at concentrations of 10–100 µg/mL.
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spelling pubmed-79986992021-03-28 Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles Brennan, Grace Ryan, Sally Soulimane, Tewfik Tofail, Syed A. M. Silien, Christophe Nanomaterials (Basel) Article Magnetic-plasmonic, Fe(3)O(4)-Au, core-shell nanoparticles are popular in many applications, most notably in therapeutics and diagnostics, and thus, the imaging of these nanostructures in biological samples is of high importance. These nanostructures are typically imaged in biological material by dark field scatter imaging, which requires an even distribution of nanostructures in the sample and, therefore, high nanoparticle doses, potentially leading to toxicology issues. Herein, we explore the nonlinear optical properties of magnetic nanoparticles coated with various thicknesses of gold using the open aperture z-scan technique to determine the nonlinear optical properties and moreover, predict the efficacy of the nanostructures in nonlinear imaging. We find that the magnetic nanoparticles coated with gold nanoseeds and thinner gold shells (ca. 4 nm) show the largest nonlinear absorption coefficient β and imaginary part of the third-order susceptibility Im χ((3)), suggesting that these nanostructures would be suitable contrast agents. Next, we combine laser dark field microscopy and epi-detected coherent anti-Stokes Raman (CARS) microscopy to image the uptake of magnetic-plasmonic nanoparticles in human pancreatic cancer cells. We show the epi-detected CARS technique is suitable for imaging of the magnetic-plasmonic nanoparticles without requiring a dense distribution of nanoparticles. This technique achieves superior nanoparticle contrasting over both epi-detected backscatter imaging and transmission dark field imaging, while also attaining label-free chemical contrasting of the cell. Lastly, we show the high biocompatibility of the Fe(3)O(4) nanoparticles with ca. 4-nm thick Au shell at concentrations of 10–100 µg/mL. MDPI 2021-03-09 /pmc/articles/PMC7998699/ /pubmed/33803430 http://dx.doi.org/10.3390/nano11030685 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Brennan, Grace
Ryan, Sally
Soulimane, Tewfik
Tofail, Syed A. M.
Silien, Christophe
Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles
title Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles
title_full Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles
title_fullStr Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles
title_full_unstemmed Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles
title_short Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles
title_sort dark field and coherent anti-stokes raman (df-cars) imaging of cell uptake of core-shell, magnetic-plasmonic nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998699/
https://www.ncbi.nlm.nih.gov/pubmed/33803430
http://dx.doi.org/10.3390/nano11030685
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