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Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes

SIMPLE SUMMARY: Melanoma is one of the most aggressive cancers in humans, with high rates of metastasis and a poor prognosis. Because of its environmental, biological and genetic features, numerous studies indicate the dog as a good comparative model for human melanoma. Primary cell cultures of heal...

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Autores principales: Sforna, Monica, Chiaradia, Elisabetta, Porcellato, Ilaria, Silvestri, Serenella, Moretti, Giulia, Mechelli, Luca, Brachelente, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998744/
https://www.ncbi.nlm.nih.gov/pubmed/33802040
http://dx.doi.org/10.3390/ani11030768
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author Sforna, Monica
Chiaradia, Elisabetta
Porcellato, Ilaria
Silvestri, Serenella
Moretti, Giulia
Mechelli, Luca
Brachelente, Chiara
author_facet Sforna, Monica
Chiaradia, Elisabetta
Porcellato, Ilaria
Silvestri, Serenella
Moretti, Giulia
Mechelli, Luca
Brachelente, Chiara
author_sort Sforna, Monica
collection PubMed
description SIMPLE SUMMARY: Melanoma is one of the most aggressive cancers in humans, with high rates of metastasis and a poor prognosis. Because of its environmental, biological and genetic features, numerous studies indicate the dog as a good comparative model for human melanoma. Primary cell cultures of healthy and neoplastic melanocytes derived from skin and oral mucosa of dogs with spontaneous tumors are established in this study. This model could represent a suitable tool to compare biological and molecular features of normal and neoplastic melanocytes from the same patient, to investigate the pathways underlying the oncogenic transformation, and to apply a more personalized therapeutic strategy. The cell cultures also meet international guidelines that encourage the use of alternative models to animal ones for the study of oncological diseases. ABSTRACT: Although numerous animal models, especially mouse models, have been established for the study of melanoma, they often fail to accurately describe the mechanisms of human disease because of their anatomic, physiological, and immune differences. The dog, as a spontaneous model of melanoma, is nowadays considered one of the most valid alternatives due to the heterogeneity of clinical presentations and of histological and genetic similarities of canine melanoma with the human counterpart. The aim of the study was to optimize a protocol for the isolation and cultivation of healthy and neoplastic canine melanocytes derived from the same animal and obtained from cutaneous and mucosal (oral) sites. We obtained five primary tumor cell cultures (from 2 cutaneous melanoma, 2 mucosal melanoma and 1 lymph node metastasis) and primary normal melanocyte cell cultures (from normal skin and mucosa) from the same dogs. Immunocytochemical characterization with Melan A, PNL2 and S100 antibodies confirmed the melanocytic origin of the cells. This work contributes to expanding the case record of studies on canine melanoma cell cultures as suitable model to study human and canine melanoma. To the authors’ knowledge, this is the first report of isolation of normal skin and mucosal canine melanocytes.
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spelling pubmed-79987442021-03-28 Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes Sforna, Monica Chiaradia, Elisabetta Porcellato, Ilaria Silvestri, Serenella Moretti, Giulia Mechelli, Luca Brachelente, Chiara Animals (Basel) Article SIMPLE SUMMARY: Melanoma is one of the most aggressive cancers in humans, with high rates of metastasis and a poor prognosis. Because of its environmental, biological and genetic features, numerous studies indicate the dog as a good comparative model for human melanoma. Primary cell cultures of healthy and neoplastic melanocytes derived from skin and oral mucosa of dogs with spontaneous tumors are established in this study. This model could represent a suitable tool to compare biological and molecular features of normal and neoplastic melanocytes from the same patient, to investigate the pathways underlying the oncogenic transformation, and to apply a more personalized therapeutic strategy. The cell cultures also meet international guidelines that encourage the use of alternative models to animal ones for the study of oncological diseases. ABSTRACT: Although numerous animal models, especially mouse models, have been established for the study of melanoma, they often fail to accurately describe the mechanisms of human disease because of their anatomic, physiological, and immune differences. The dog, as a spontaneous model of melanoma, is nowadays considered one of the most valid alternatives due to the heterogeneity of clinical presentations and of histological and genetic similarities of canine melanoma with the human counterpart. The aim of the study was to optimize a protocol for the isolation and cultivation of healthy and neoplastic canine melanocytes derived from the same animal and obtained from cutaneous and mucosal (oral) sites. We obtained five primary tumor cell cultures (from 2 cutaneous melanoma, 2 mucosal melanoma and 1 lymph node metastasis) and primary normal melanocyte cell cultures (from normal skin and mucosa) from the same dogs. Immunocytochemical characterization with Melan A, PNL2 and S100 antibodies confirmed the melanocytic origin of the cells. This work contributes to expanding the case record of studies on canine melanoma cell cultures as suitable model to study human and canine melanoma. To the authors’ knowledge, this is the first report of isolation of normal skin and mucosal canine melanocytes. MDPI 2021-03-10 /pmc/articles/PMC7998744/ /pubmed/33802040 http://dx.doi.org/10.3390/ani11030768 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Sforna, Monica
Chiaradia, Elisabetta
Porcellato, Ilaria
Silvestri, Serenella
Moretti, Giulia
Mechelli, Luca
Brachelente, Chiara
Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes
title Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes
title_full Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes
title_fullStr Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes
title_full_unstemmed Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes
title_short Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes
title_sort characterization of primary cultures of normal and neoplastic canine melanocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998744/
https://www.ncbi.nlm.nih.gov/pubmed/33802040
http://dx.doi.org/10.3390/ani11030768
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