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Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting

Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner d...

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Autores principales: Klak, Marta, Kowalska, Patrycja, Dobrzański, Tomasz, Tymicki, Grzegorz, Cywoniuk, Piotr, Gomółka, Magdalena, Kosowska, Katarzyna, Bryniarski, Tomasz, Berman, Andrzej, Dobrzyń, Agnieszka, Sadowski, Wojciech, Górecki, Bartosz, Wszoła, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999205/
https://www.ncbi.nlm.nih.gov/pubmed/33799490
http://dx.doi.org/10.3390/mi12030304
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author Klak, Marta
Kowalska, Patrycja
Dobrzański, Tomasz
Tymicki, Grzegorz
Cywoniuk, Piotr
Gomółka, Magdalena
Kosowska, Katarzyna
Bryniarski, Tomasz
Berman, Andrzej
Dobrzyń, Agnieszka
Sadowski, Wojciech
Górecki, Bartosz
Wszoła, Michał
author_facet Klak, Marta
Kowalska, Patrycja
Dobrzański, Tomasz
Tymicki, Grzegorz
Cywoniuk, Piotr
Gomółka, Magdalena
Kosowska, Katarzyna
Bryniarski, Tomasz
Berman, Andrzej
Dobrzyń, Agnieszka
Sadowski, Wojciech
Górecki, Bartosz
Wszoła, Michał
author_sort Klak, Marta
collection PubMed
description Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (w/v) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (w/v) alginate as a carrier.
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spelling pubmed-79992052021-03-28 Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting Klak, Marta Kowalska, Patrycja Dobrzański, Tomasz Tymicki, Grzegorz Cywoniuk, Piotr Gomółka, Magdalena Kosowska, Katarzyna Bryniarski, Tomasz Berman, Andrzej Dobrzyń, Agnieszka Sadowski, Wojciech Górecki, Bartosz Wszoła, Michał Micromachines (Basel) Article Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (w/v) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (w/v) alginate as a carrier. MDPI 2021-03-14 /pmc/articles/PMC7999205/ /pubmed/33799490 http://dx.doi.org/10.3390/mi12030304 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Klak, Marta
Kowalska, Patrycja
Dobrzański, Tomasz
Tymicki, Grzegorz
Cywoniuk, Piotr
Gomółka, Magdalena
Kosowska, Katarzyna
Bryniarski, Tomasz
Berman, Andrzej
Dobrzyń, Agnieszka
Sadowski, Wojciech
Górecki, Bartosz
Wszoła, Michał
Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
title Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
title_full Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
title_fullStr Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
title_full_unstemmed Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
title_short Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
title_sort bionic organs: shear forces reduce pancreatic islet and mammalian cell viability during the process of 3d bioprinting
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999205/
https://www.ncbi.nlm.nih.gov/pubmed/33799490
http://dx.doi.org/10.3390/mi12030304
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