Cargando…
Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner d...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999205/ https://www.ncbi.nlm.nih.gov/pubmed/33799490 http://dx.doi.org/10.3390/mi12030304 |
_version_ | 1783670728240922624 |
---|---|
author | Klak, Marta Kowalska, Patrycja Dobrzański, Tomasz Tymicki, Grzegorz Cywoniuk, Piotr Gomółka, Magdalena Kosowska, Katarzyna Bryniarski, Tomasz Berman, Andrzej Dobrzyń, Agnieszka Sadowski, Wojciech Górecki, Bartosz Wszoła, Michał |
author_facet | Klak, Marta Kowalska, Patrycja Dobrzański, Tomasz Tymicki, Grzegorz Cywoniuk, Piotr Gomółka, Magdalena Kosowska, Katarzyna Bryniarski, Tomasz Berman, Andrzej Dobrzyń, Agnieszka Sadowski, Wojciech Górecki, Bartosz Wszoła, Michał |
author_sort | Klak, Marta |
collection | PubMed |
description | Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (w/v) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (w/v) alginate as a carrier. |
format | Online Article Text |
id | pubmed-7999205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79992052021-03-28 Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting Klak, Marta Kowalska, Patrycja Dobrzański, Tomasz Tymicki, Grzegorz Cywoniuk, Piotr Gomółka, Magdalena Kosowska, Katarzyna Bryniarski, Tomasz Berman, Andrzej Dobrzyń, Agnieszka Sadowski, Wojciech Górecki, Bartosz Wszoła, Michał Micromachines (Basel) Article Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (w/v) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (w/v) alginate as a carrier. MDPI 2021-03-14 /pmc/articles/PMC7999205/ /pubmed/33799490 http://dx.doi.org/10.3390/mi12030304 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Klak, Marta Kowalska, Patrycja Dobrzański, Tomasz Tymicki, Grzegorz Cywoniuk, Piotr Gomółka, Magdalena Kosowska, Katarzyna Bryniarski, Tomasz Berman, Andrzej Dobrzyń, Agnieszka Sadowski, Wojciech Górecki, Bartosz Wszoła, Michał Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
title | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
title_full | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
title_fullStr | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
title_full_unstemmed | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
title_short | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
title_sort | bionic organs: shear forces reduce pancreatic islet and mammalian cell viability during the process of 3d bioprinting |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999205/ https://www.ncbi.nlm.nih.gov/pubmed/33799490 http://dx.doi.org/10.3390/mi12030304 |
work_keys_str_mv | AT klakmarta bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT kowalskapatrycja bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT dobrzanskitomasz bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT tymickigrzegorz bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT cywoniukpiotr bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT gomołkamagdalena bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT kosowskakatarzyna bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT bryniarskitomasz bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT bermanandrzej bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT dobrzynagnieszka bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT sadowskiwojciech bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT goreckibartosz bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting AT wszołamichał bionicorgansshearforcesreducepancreaticisletandmammaliancellviabilityduringtheprocessof3dbioprinting |