Cargando…

The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies

Mycosphaerellaceae is a highly diverse fungal family containing a variety of pathogens affecting many economically important crops. Mitochondria play a crucial role in fungal metabolism and in the study of fungal evolution. This study aims to: (i) describe the mitochondrial genome of Pseudocercospor...

Descripción completa

Detalles Bibliográficos
Autores principales: Arcila-Galvis, Juliana E., Arango, Rafael E., Torres-Bonilla, Javier M., Arias, Tatiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999263/
https://www.ncbi.nlm.nih.gov/pubmed/33803147
http://dx.doi.org/10.3390/life11030215
_version_ 1783670741908062208
author Arcila-Galvis, Juliana E.
Arango, Rafael E.
Torres-Bonilla, Javier M.
Arias, Tatiana
author_facet Arcila-Galvis, Juliana E.
Arango, Rafael E.
Torres-Bonilla, Javier M.
Arias, Tatiana
author_sort Arcila-Galvis, Juliana E.
collection PubMed
description Mycosphaerellaceae is a highly diverse fungal family containing a variety of pathogens affecting many economically important crops. Mitochondria play a crucial role in fungal metabolism and in the study of fungal evolution. This study aims to: (i) describe the mitochondrial genome of Pseudocercospora fijiensis, and (ii) compare it with closely related species (Sphaerulina musiva, S. populicola, P. musae and P. eumusae) available online, paying particular attention to the Sigatoka disease’s complex causal agents. The mitochondrial genome of P. fijiensis is a circular molecule of 74,089 bp containing typical genes coding for the 14 proteins related to oxidative phosphorylation, 2 rRNA genes and a set of 38 tRNAs. P. fijiensis mitogenome has two truncated cox1 copies, and bicistronic transcription of nad2-nad3 and atp6-atp8 confirmed experimentally. Comparative analysis revealed high variability in size and gene order among selected Mycosphaerellaceae mitogenomes likely to be due to rearrangements caused by mobile intron invasion. Using fossil calibrated Bayesian phylogenies, we found later diversification times for Mycosphaerellaceae (66.6 MYA) and the Sigatoka disease complex causal agents, compared to previous strict molecular clock studies. An early divergent Pseudocercospora fijiensis split from the sister species P. musae + P. eumusae 13.31 MYA while their sister group, the sister species P. eumusae and P. musae, split from their shared common ancestor in the late Miocene 8.22 MYA. This newly dated phylogeny suggests that species belonging to the Sigatoka disease complex originated after wild relatives of domesticated bananas (section Eumusae; 27.9 MYA). During this time frame, mitochondrial genomes expanded significantly, possibly due to invasions of introns into different electron transport chain genes.
format Online
Article
Text
id pubmed-7999263
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79992632021-03-28 The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies Arcila-Galvis, Juliana E. Arango, Rafael E. Torres-Bonilla, Javier M. Arias, Tatiana Life (Basel) Article Mycosphaerellaceae is a highly diverse fungal family containing a variety of pathogens affecting many economically important crops. Mitochondria play a crucial role in fungal metabolism and in the study of fungal evolution. This study aims to: (i) describe the mitochondrial genome of Pseudocercospora fijiensis, and (ii) compare it with closely related species (Sphaerulina musiva, S. populicola, P. musae and P. eumusae) available online, paying particular attention to the Sigatoka disease’s complex causal agents. The mitochondrial genome of P. fijiensis is a circular molecule of 74,089 bp containing typical genes coding for the 14 proteins related to oxidative phosphorylation, 2 rRNA genes and a set of 38 tRNAs. P. fijiensis mitogenome has two truncated cox1 copies, and bicistronic transcription of nad2-nad3 and atp6-atp8 confirmed experimentally. Comparative analysis revealed high variability in size and gene order among selected Mycosphaerellaceae mitogenomes likely to be due to rearrangements caused by mobile intron invasion. Using fossil calibrated Bayesian phylogenies, we found later diversification times for Mycosphaerellaceae (66.6 MYA) and the Sigatoka disease complex causal agents, compared to previous strict molecular clock studies. An early divergent Pseudocercospora fijiensis split from the sister species P. musae + P. eumusae 13.31 MYA while their sister group, the sister species P. eumusae and P. musae, split from their shared common ancestor in the late Miocene 8.22 MYA. This newly dated phylogeny suggests that species belonging to the Sigatoka disease complex originated after wild relatives of domesticated bananas (section Eumusae; 27.9 MYA). During this time frame, mitochondrial genomes expanded significantly, possibly due to invasions of introns into different electron transport chain genes. MDPI 2021-03-09 /pmc/articles/PMC7999263/ /pubmed/33803147 http://dx.doi.org/10.3390/life11030215 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Arcila-Galvis, Juliana E.
Arango, Rafael E.
Torres-Bonilla, Javier M.
Arias, Tatiana
The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies
title The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies
title_full The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies
title_fullStr The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies
title_full_unstemmed The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies
title_short The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies
title_sort mitochondrial genome of a plant fungal pathogen pseudocercospora fijiensis (mycosphaerellaceae), comparative analysis and diversification times of the sigatoka disease complex using fossil calibrated phylogenies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999263/
https://www.ncbi.nlm.nih.gov/pubmed/33803147
http://dx.doi.org/10.3390/life11030215
work_keys_str_mv AT arcilagalvisjulianae themitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT arangorafaele themitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT torresbonillajavierm themitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT ariastatiana themitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT arcilagalvisjulianae mitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT arangorafaele mitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT torresbonillajavierm mitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies
AT ariastatiana mitochondrialgenomeofaplantfungalpathogenpseudocercosporafijiensismycosphaerellaceaecomparativeanalysisanddiversificationtimesofthesigatokadiseasecomplexusingfossilcalibratedphylogenies