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Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections

Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, us...

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Autores principales: Mohanraj, Ushanandini, Jokinen, Maija, Thapa, Rajita Rayamajhi, Paloniemi, Minna, Vesikari, Timo, Lappalainen, Maija, Tarkka, Eveliina, Nora-Krūkle, Zaiga, Vilmane, Anda, Vettenranta, Kim, Mangani, Charles, Oikarinen, Sami, Fan, Yue-Mei, Ashorn, Per, Väisänen, Elina, Söderlund-Venermo, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999311/
https://www.ncbi.nlm.nih.gov/pubmed/33804173
http://dx.doi.org/10.3390/v13030483
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author Mohanraj, Ushanandini
Jokinen, Maija
Thapa, Rajita Rayamajhi
Paloniemi, Minna
Vesikari, Timo
Lappalainen, Maija
Tarkka, Eveliina
Nora-Krūkle, Zaiga
Vilmane, Anda
Vettenranta, Kim
Mangani, Charles
Oikarinen, Sami
Fan, Yue-Mei
Ashorn, Per
Väisänen, Elina
Söderlund-Venermo, Maria
author_facet Mohanraj, Ushanandini
Jokinen, Maija
Thapa, Rajita Rayamajhi
Paloniemi, Minna
Vesikari, Timo
Lappalainen, Maija
Tarkka, Eveliina
Nora-Krūkle, Zaiga
Vilmane, Anda
Vettenranta, Kim
Mangani, Charles
Oikarinen, Sami
Fan, Yue-Mei
Ashorn, Per
Väisänen, Elina
Söderlund-Venermo, Maria
author_sort Mohanraj, Ushanandini
collection PubMed
description Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, using qPCR and IgG enzyme immunoassays, we analyzed 1072 stool, 316 respiratory and 445 serum or plasma samples from 1098 patients with and without gastroenteritis (GE) or respiratory-tract infections (RTI) from Finland, Latvia and Malawi. The overall CuV-DNA prevalences in stool samples ranged between 0–6.1% among our six patient cohorts. In Finland, CuV DNA was significantly more prevalent in GE patients above rather than below 60 years of age (5.1% vs 0.2%). CuV DNA was more prevalent in stools among Latvian and Malawian children compared with Finnish children. In 10/11 CuV DNA-positive adults and 4/6 CuV DNA-positive children with GE, no known causal pathogens were detected. Interestingly, for the first time, CuV DNA was observed in two nasopharyngeal aspirates from children with RTI and the rare TuV in diarrheal stools of two adults. Our results provide new insights on the occurrence of human protoparvoviruses in GE and RTI in different countries.
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spelling pubmed-79993112021-03-28 Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections Mohanraj, Ushanandini Jokinen, Maija Thapa, Rajita Rayamajhi Paloniemi, Minna Vesikari, Timo Lappalainen, Maija Tarkka, Eveliina Nora-Krūkle, Zaiga Vilmane, Anda Vettenranta, Kim Mangani, Charles Oikarinen, Sami Fan, Yue-Mei Ashorn, Per Väisänen, Elina Söderlund-Venermo, Maria Viruses Article Three human protoparvoviruses, bufavirus (BuV), tusavirus (TuV) and cutavirus (CuV), have recently been discovered in diarrheal stool. BuV has been associated with diarrhea and CuV with cutaneous T-cell lymphoma, but there are hardly any data for TuV or CuV in stool or respiratory samples. Hence, using qPCR and IgG enzyme immunoassays, we analyzed 1072 stool, 316 respiratory and 445 serum or plasma samples from 1098 patients with and without gastroenteritis (GE) or respiratory-tract infections (RTI) from Finland, Latvia and Malawi. The overall CuV-DNA prevalences in stool samples ranged between 0–6.1% among our six patient cohorts. In Finland, CuV DNA was significantly more prevalent in GE patients above rather than below 60 years of age (5.1% vs 0.2%). CuV DNA was more prevalent in stools among Latvian and Malawian children compared with Finnish children. In 10/11 CuV DNA-positive adults and 4/6 CuV DNA-positive children with GE, no known causal pathogens were detected. Interestingly, for the first time, CuV DNA was observed in two nasopharyngeal aspirates from children with RTI and the rare TuV in diarrheal stools of two adults. Our results provide new insights on the occurrence of human protoparvoviruses in GE and RTI in different countries. MDPI 2021-03-15 /pmc/articles/PMC7999311/ /pubmed/33804173 http://dx.doi.org/10.3390/v13030483 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Mohanraj, Ushanandini
Jokinen, Maija
Thapa, Rajita Rayamajhi
Paloniemi, Minna
Vesikari, Timo
Lappalainen, Maija
Tarkka, Eveliina
Nora-Krūkle, Zaiga
Vilmane, Anda
Vettenranta, Kim
Mangani, Charles
Oikarinen, Sami
Fan, Yue-Mei
Ashorn, Per
Väisänen, Elina
Söderlund-Venermo, Maria
Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
title Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
title_full Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
title_fullStr Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
title_full_unstemmed Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
title_short Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections
title_sort human protoparvovirus dna and igg in children and adults with and without respiratory or gastrointestinal infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999311/
https://www.ncbi.nlm.nih.gov/pubmed/33804173
http://dx.doi.org/10.3390/v13030483
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