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Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)

In 2020, three articles were published on a protein that can activate the immune system by binding to macrophage-inducible C-type lectin receptor (Mincle). In the articles, the protein was referred to as ‘SAP130, a subunit of the histone deacetylase complex.’ However, the Mincle ligand the authors a...

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Autores principales: Metselaar, Paula I., Hos, Celine, Welting, Olaf, Bosch, Jos A., Kraneveld, Aletta D., de Jonge, Wouter J., Te Velde, Anje A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999425/
https://www.ncbi.nlm.nih.gov/pubmed/33800128
http://dx.doi.org/10.3390/cells10030590
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author Metselaar, Paula I.
Hos, Celine
Welting, Olaf
Bosch, Jos A.
Kraneveld, Aletta D.
de Jonge, Wouter J.
Te Velde, Anje A.
author_facet Metselaar, Paula I.
Hos, Celine
Welting, Olaf
Bosch, Jos A.
Kraneveld, Aletta D.
de Jonge, Wouter J.
Te Velde, Anje A.
author_sort Metselaar, Paula I.
collection PubMed
description In 2020, three articles were published on a protein that can activate the immune system by binding to macrophage-inducible C-type lectin receptor (Mincle). In the articles, the protein was referred to as ‘SAP130, a subunit of the histone deacetylase complex.’ However, the Mincle ligand the authors aimed to investigate is splicing factor 3b subunit 3 (SF3B3). This splicing factor is unrelated to SAP130 (Sin3A associated protein 130, a subunit of the histone deacetylase-dependent Sin3A corepressor complex). The conclusions in the three articles were formulated for SF3B3, while the researchers used qPCR primers and antibodies against SAP130. We retraced the origins of the ambiguity about the two proteins and found that Online Mendelian Inheritance in Man (OMIM) added a Nature publication on SF3B3 as a reference for Sin3A associated protein 130 in 2016. Subsequently, companies such as Abcam referred to OMIM and the Nature article in their products for both SF3B3 and SAP130. In turn, the mistake by OMIM followed in the persistent and confusing use of ‘SAP130′ (spliceosome-associated protein 130) as an alternative symbol for SF3B3. With this report, we aim to eliminate the persistent confusion and separate the literature regarding the two proteins.
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spelling pubmed-79994252021-03-28 Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) Metselaar, Paula I. Hos, Celine Welting, Olaf Bosch, Jos A. Kraneveld, Aletta D. de Jonge, Wouter J. Te Velde, Anje A. Cells Communication In 2020, three articles were published on a protein that can activate the immune system by binding to macrophage-inducible C-type lectin receptor (Mincle). In the articles, the protein was referred to as ‘SAP130, a subunit of the histone deacetylase complex.’ However, the Mincle ligand the authors aimed to investigate is splicing factor 3b subunit 3 (SF3B3). This splicing factor is unrelated to SAP130 (Sin3A associated protein 130, a subunit of the histone deacetylase-dependent Sin3A corepressor complex). The conclusions in the three articles were formulated for SF3B3, while the researchers used qPCR primers and antibodies against SAP130. We retraced the origins of the ambiguity about the two proteins and found that Online Mendelian Inheritance in Man (OMIM) added a Nature publication on SF3B3 as a reference for Sin3A associated protein 130 in 2016. Subsequently, companies such as Abcam referred to OMIM and the Nature article in their products for both SF3B3 and SAP130. In turn, the mistake by OMIM followed in the persistent and confusing use of ‘SAP130′ (spliceosome-associated protein 130) as an alternative symbol for SF3B3. With this report, we aim to eliminate the persistent confusion and separate the literature regarding the two proteins. MDPI 2021-03-08 /pmc/articles/PMC7999425/ /pubmed/33800128 http://dx.doi.org/10.3390/cells10030590 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Communication
Metselaar, Paula I.
Hos, Celine
Welting, Olaf
Bosch, Jos A.
Kraneveld, Aletta D.
de Jonge, Wouter J.
Te Velde, Anje A.
Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
title Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
title_full Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
title_fullStr Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
title_full_unstemmed Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
title_short Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
title_sort ambiguity about splicing factor 3b subunit 3 (sf3b3) and sin3a associated protein 130 (sap130)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999425/
https://www.ncbi.nlm.nih.gov/pubmed/33800128
http://dx.doi.org/10.3390/cells10030590
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