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COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020

COVID-19 pandemic is a dramatic health, social and economic global challenge. There is urgent need to maximize testing capacity. Rapid Antigen Tests (RAT) represent good candidates for point-of-care and mass surveillance testing to rapidly identify SARS-CoV-2-infected people, counterbalancing lower...

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Autores principales: Colavita, Francesca, Vairo, Francesco, Meschi, Silvia, Valli, Maria Beatrice, Lalle, Eleonora, Castilletti, Concetta, Fusco, Danilo, Spiga, Giuseppe, Bartoletti, Pierluigi, Ursino, Simona, Sanguinetti, Maurizio, Di Caro, Antonino, Vaia, Francesco, Ippolito, Giuseppe, Capobianchi, Maria Rosaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999510/
https://www.ncbi.nlm.nih.gov/pubmed/33805832
http://dx.doi.org/10.3390/biom11030425
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author Colavita, Francesca
Vairo, Francesco
Meschi, Silvia
Valli, Maria Beatrice
Lalle, Eleonora
Castilletti, Concetta
Fusco, Danilo
Spiga, Giuseppe
Bartoletti, Pierluigi
Ursino, Simona
Sanguinetti, Maurizio
Di Caro, Antonino
Vaia, Francesco
Ippolito, Giuseppe
Capobianchi, Maria Rosaria
author_facet Colavita, Francesca
Vairo, Francesco
Meschi, Silvia
Valli, Maria Beatrice
Lalle, Eleonora
Castilletti, Concetta
Fusco, Danilo
Spiga, Giuseppe
Bartoletti, Pierluigi
Ursino, Simona
Sanguinetti, Maurizio
Di Caro, Antonino
Vaia, Francesco
Ippolito, Giuseppe
Capobianchi, Maria Rosaria
author_sort Colavita, Francesca
collection PubMed
description COVID-19 pandemic is a dramatic health, social and economic global challenge. There is urgent need to maximize testing capacity. Rapid Antigen Tests (RAT) represent good candidates for point-of-care and mass surveillance testing to rapidly identify SARS-CoV-2-infected people, counterbalancing lower sensitivity vs. gold standard molecular tests with fast results and possible recurrent testing. We describe the results obtained with the testing algorithm implemented at points of entry (airports and ports) in the Lazio Region (Italy), using the STANDARD F COVID-19 Antigen Fluorescence ImmunoAssay (FIA), followed by molecular confirmation of FIA-positive samples. From mid-August to mid-October 2020, 73,643 RAT were reported to the Regional Surveillance Information System for travelers at points of entry in Lazio Region. Of these, 1176 (1.6%) were FIA-positive, and the proportion of RT-PCR-confirmed samples was 40.5%. Our data show that the probability of confirmation was directly dependent from the semi-quantitative FIA results. In addition, the molecularly confirmed samples were those with high levels of virus and that were actually harboring infectious virus. These results support public health strategies based on early mass screening campaigns by RAT in settings where molecular testing is not feasible or easily accessible, such as points of entry. This approach would contribute to promptly controlling viral spread through travel, which is now of particular concern due to the spread of SARS-CoV-2 variants.
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spelling pubmed-79995102021-03-28 COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020 Colavita, Francesca Vairo, Francesco Meschi, Silvia Valli, Maria Beatrice Lalle, Eleonora Castilletti, Concetta Fusco, Danilo Spiga, Giuseppe Bartoletti, Pierluigi Ursino, Simona Sanguinetti, Maurizio Di Caro, Antonino Vaia, Francesco Ippolito, Giuseppe Capobianchi, Maria Rosaria Biomolecules Article COVID-19 pandemic is a dramatic health, social and economic global challenge. There is urgent need to maximize testing capacity. Rapid Antigen Tests (RAT) represent good candidates for point-of-care and mass surveillance testing to rapidly identify SARS-CoV-2-infected people, counterbalancing lower sensitivity vs. gold standard molecular tests with fast results and possible recurrent testing. We describe the results obtained with the testing algorithm implemented at points of entry (airports and ports) in the Lazio Region (Italy), using the STANDARD F COVID-19 Antigen Fluorescence ImmunoAssay (FIA), followed by molecular confirmation of FIA-positive samples. From mid-August to mid-October 2020, 73,643 RAT were reported to the Regional Surveillance Information System for travelers at points of entry in Lazio Region. Of these, 1176 (1.6%) were FIA-positive, and the proportion of RT-PCR-confirmed samples was 40.5%. Our data show that the probability of confirmation was directly dependent from the semi-quantitative FIA results. In addition, the molecularly confirmed samples were those with high levels of virus and that were actually harboring infectious virus. These results support public health strategies based on early mass screening campaigns by RAT in settings where molecular testing is not feasible or easily accessible, such as points of entry. This approach would contribute to promptly controlling viral spread through travel, which is now of particular concern due to the spread of SARS-CoV-2 variants. MDPI 2021-03-13 /pmc/articles/PMC7999510/ /pubmed/33805832 http://dx.doi.org/10.3390/biom11030425 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Colavita, Francesca
Vairo, Francesco
Meschi, Silvia
Valli, Maria Beatrice
Lalle, Eleonora
Castilletti, Concetta
Fusco, Danilo
Spiga, Giuseppe
Bartoletti, Pierluigi
Ursino, Simona
Sanguinetti, Maurizio
Di Caro, Antonino
Vaia, Francesco
Ippolito, Giuseppe
Capobianchi, Maria Rosaria
COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
title COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
title_full COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
title_fullStr COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
title_full_unstemmed COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
title_short COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020
title_sort covid-19 rapid antigen test as screening strategy at points of entry: experience in lazio region, central italy, august–october 2020
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999510/
https://www.ncbi.nlm.nih.gov/pubmed/33805832
http://dx.doi.org/10.3390/biom11030425
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