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Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France

Puumala orthohantavirus (PUUV) causes a mild form of haemorrhagic fever with renal syndrome (HFRS) called nephropathia epidemica (NE), regularly diagnosed in Europe. France represents the western frontier of the expansion of NE in Europe with two distinct areas: an endemic area (north-eastern France...

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Autores principales: Vulin, Johann, Murri, Séverine, Madrières, Sarah, Galan, Maxime, Tatard, Caroline, Piry, Sylvain, Vaccari, Gabriele, D’Agostino, Claudia, Charbonnel, Nathalie, Castel, Guillaume, Marianneau, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999636/
https://www.ncbi.nlm.nih.gov/pubmed/33809526
http://dx.doi.org/10.3390/pathogens10030349
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author Vulin, Johann
Murri, Séverine
Madrières, Sarah
Galan, Maxime
Tatard, Caroline
Piry, Sylvain
Vaccari, Gabriele
D’Agostino, Claudia
Charbonnel, Nathalie
Castel, Guillaume
Marianneau, Philippe
author_facet Vulin, Johann
Murri, Séverine
Madrières, Sarah
Galan, Maxime
Tatard, Caroline
Piry, Sylvain
Vaccari, Gabriele
D’Agostino, Claudia
Charbonnel, Nathalie
Castel, Guillaume
Marianneau, Philippe
author_sort Vulin, Johann
collection PubMed
description Puumala orthohantavirus (PUUV) causes a mild form of haemorrhagic fever with renal syndrome (HFRS) called nephropathia epidemica (NE), regularly diagnosed in Europe. France represents the western frontier of the expansion of NE in Europe with two distinct areas: an endemic area (north-eastern France) where PUUV circulates in rodent populations, with the detection of many human NE cases, and a non-endemic area (south-western France) where the virus is not detected, with only a few human cases being reported. In this study, we describe the different stages of the isolation of two PUUV strains from two distinct French geographical areas: Ardennes (endemic area) and Loiret (non-endemic area). To isolate PUUV efficiently, we selected wild bank voles (Myodes glareolus, the specific reservoir of PUUV) captured in these areas and that were seronegative for anti-PUUV IgG (ELISA) but showed a non-negligible viral RNA load in their lung tissue (qRT-PCR). With this study design, we were able to cultivate and maintain these two strains in Vero E6 cells and also propagate both strains in immunologically neutral bank voles efficiently and rapidly. High-throughput and Sanger sequencing results provided a better assessment of the impact of isolation methods on viral diversity.
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spelling pubmed-79996362021-03-28 Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France Vulin, Johann Murri, Séverine Madrières, Sarah Galan, Maxime Tatard, Caroline Piry, Sylvain Vaccari, Gabriele D’Agostino, Claudia Charbonnel, Nathalie Castel, Guillaume Marianneau, Philippe Pathogens Communication Puumala orthohantavirus (PUUV) causes a mild form of haemorrhagic fever with renal syndrome (HFRS) called nephropathia epidemica (NE), regularly diagnosed in Europe. France represents the western frontier of the expansion of NE in Europe with two distinct areas: an endemic area (north-eastern France) where PUUV circulates in rodent populations, with the detection of many human NE cases, and a non-endemic area (south-western France) where the virus is not detected, with only a few human cases being reported. In this study, we describe the different stages of the isolation of two PUUV strains from two distinct French geographical areas: Ardennes (endemic area) and Loiret (non-endemic area). To isolate PUUV efficiently, we selected wild bank voles (Myodes glareolus, the specific reservoir of PUUV) captured in these areas and that were seronegative for anti-PUUV IgG (ELISA) but showed a non-negligible viral RNA load in their lung tissue (qRT-PCR). With this study design, we were able to cultivate and maintain these two strains in Vero E6 cells and also propagate both strains in immunologically neutral bank voles efficiently and rapidly. High-throughput and Sanger sequencing results provided a better assessment of the impact of isolation methods on viral diversity. MDPI 2021-03-16 /pmc/articles/PMC7999636/ /pubmed/33809526 http://dx.doi.org/10.3390/pathogens10030349 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Communication
Vulin, Johann
Murri, Séverine
Madrières, Sarah
Galan, Maxime
Tatard, Caroline
Piry, Sylvain
Vaccari, Gabriele
D’Agostino, Claudia
Charbonnel, Nathalie
Castel, Guillaume
Marianneau, Philippe
Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France
title Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France
title_full Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France
title_fullStr Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France
title_full_unstemmed Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France
title_short Isolation and Genetic Characterization of Puumala Orthohantavirus Strains from France
title_sort isolation and genetic characterization of puumala orthohantavirus strains from france
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999636/
https://www.ncbi.nlm.nih.gov/pubmed/33809526
http://dx.doi.org/10.3390/pathogens10030349
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