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Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia
Despite their distinct clinical manifestation, frontal fibrosing alopecia (FFA) and lichen planopilaris (LPP) display similar histopathologic features. Aberrant innate immune responses to endogenous or exogenous triggers have been discussed as factors that could drive inflammatory cascades and the c...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999846/ https://www.ncbi.nlm.nih.gov/pubmed/33800045 http://dx.doi.org/10.3390/biomedicines9030266 |
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author | Constantinou, Andria Polak-Witka, Katarzyna Tomazou, Marios Oulas, Anastasis Kanti, Varvara Schwarzer, Rolf Helmuth, Johannes Edelmann, Anke Blume-Peytavi, Ulrike Spyrou, George M. Vogt, Annika |
author_facet | Constantinou, Andria Polak-Witka, Katarzyna Tomazou, Marios Oulas, Anastasis Kanti, Varvara Schwarzer, Rolf Helmuth, Johannes Edelmann, Anke Blume-Peytavi, Ulrike Spyrou, George M. Vogt, Annika |
author_sort | Constantinou, Andria |
collection | PubMed |
description | Despite their distinct clinical manifestation, frontal fibrosing alopecia (FFA) and lichen planopilaris (LPP) display similar histopathologic features. Aberrant innate immune responses to endogenous or exogenous triggers have been discussed as factors that could drive inflammatory cascades and the collapse of the stem cell niche. In this exploratory study, we investigate the bacterial composition of scalp skin and plucked hair follicles (HF) of patients with FFA, LPP and alopecia areata circumscripta (AAc), as well as healthy individuals, in relation to cellular infiltrates and the expression of defense mediators. The most abundant genus in lesional and non-lesional HFs of LPP and FFA patients was Staphylococcus, while Lawsonella dominated in healthy individuals and in AAc patients. We observed statistically significant differences in the ratio of Firmicutes to Actinobacteria between healthy scalp, lesional, and non-lesional sites of FFA and LPP patients. This marked dysbiosis in FFA and LPP in compartments close to the bulge was associated with increased HβD1 and HβD2 expression along the HFs from lesional sites, while IL-17A was increased in lesional HF from AAc patients. The data encourage further studies on how exogenous factors and molecular interactions across the HF epithelium could contribute to disease onset and propagation. |
format | Online Article Text |
id | pubmed-7999846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79998462021-03-28 Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia Constantinou, Andria Polak-Witka, Katarzyna Tomazou, Marios Oulas, Anastasis Kanti, Varvara Schwarzer, Rolf Helmuth, Johannes Edelmann, Anke Blume-Peytavi, Ulrike Spyrou, George M. Vogt, Annika Biomedicines Article Despite their distinct clinical manifestation, frontal fibrosing alopecia (FFA) and lichen planopilaris (LPP) display similar histopathologic features. Aberrant innate immune responses to endogenous or exogenous triggers have been discussed as factors that could drive inflammatory cascades and the collapse of the stem cell niche. In this exploratory study, we investigate the bacterial composition of scalp skin and plucked hair follicles (HF) of patients with FFA, LPP and alopecia areata circumscripta (AAc), as well as healthy individuals, in relation to cellular infiltrates and the expression of defense mediators. The most abundant genus in lesional and non-lesional HFs of LPP and FFA patients was Staphylococcus, while Lawsonella dominated in healthy individuals and in AAc patients. We observed statistically significant differences in the ratio of Firmicutes to Actinobacteria between healthy scalp, lesional, and non-lesional sites of FFA and LPP patients. This marked dysbiosis in FFA and LPP in compartments close to the bulge was associated with increased HβD1 and HβD2 expression along the HFs from lesional sites, while IL-17A was increased in lesional HF from AAc patients. The data encourage further studies on how exogenous factors and molecular interactions across the HF epithelium could contribute to disease onset and propagation. MDPI 2021-03-07 /pmc/articles/PMC7999846/ /pubmed/33800045 http://dx.doi.org/10.3390/biomedicines9030266 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Constantinou, Andria Polak-Witka, Katarzyna Tomazou, Marios Oulas, Anastasis Kanti, Varvara Schwarzer, Rolf Helmuth, Johannes Edelmann, Anke Blume-Peytavi, Ulrike Spyrou, George M. Vogt, Annika Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia |
title | Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia |
title_full | Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia |
title_fullStr | Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia |
title_full_unstemmed | Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia |
title_short | Dysbiosis and Enhanced Beta-Defensin Production in Hair Follicles of Patients with Lichen Planopilaris and Frontal Fibrosing Alopecia |
title_sort | dysbiosis and enhanced beta-defensin production in hair follicles of patients with lichen planopilaris and frontal fibrosing alopecia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999846/ https://www.ncbi.nlm.nih.gov/pubmed/33800045 http://dx.doi.org/10.3390/biomedicines9030266 |
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