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Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Collagen possesses unique properties, e.g., biocompatibility, biodegradability, and non-toxicity. However, collagen material degrades too quickly and has low mechanical properties. One of the methods of polymers’ modification is mixing them to obtain blends. In this study, the influence of β-glucan...

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Autores principales: Michalska-Sionkowska, Marta, Warżyńska, Oliwia, Kaczmarek-Szczepańska, Beata, Łukowicz, Krzysztof, Osyczka, Anna Maria, Walczak, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000014/
https://www.ncbi.nlm.nih.gov/pubmed/33801809
http://dx.doi.org/10.3390/ma14061322
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author Michalska-Sionkowska, Marta
Warżyńska, Oliwia
Kaczmarek-Szczepańska, Beata
Łukowicz, Krzysztof
Osyczka, Anna Maria
Walczak, Maciej
author_facet Michalska-Sionkowska, Marta
Warżyńska, Oliwia
Kaczmarek-Szczepańska, Beata
Łukowicz, Krzysztof
Osyczka, Anna Maria
Walczak, Maciej
author_sort Michalska-Sionkowska, Marta
collection PubMed
description Collagen possesses unique properties, e.g., biocompatibility, biodegradability, and non-toxicity. However, collagen material degrades too quickly and has low mechanical properties. One of the methods of polymers’ modification is mixing them to obtain blends. In this study, the influence of β-glucan for collagen material was analyzed. The interaction between the functional groups of the polymer was analyzed by ATR-FTIR (attenuated total reflection-fourier transform infrared) spectroscopy. The influence of β-glucan on mechanical properties was evaluated. The surface properties of materials were assessed using contact angle measurements and the topography of materials was evaluated by AFM (atomic force microscope). The structure of materials was analyzed according to SEM (scanning electron microscopy) pictures. Moreover, the DPPH-free radicals’ scavenging ability and biocompatibility against erythrocytes and HaCaT cells were evaluated. Collagen and β-glucan were bound together by a hydrogen bond. β-glucan addition increased the roughness of the surface of the film and resulted in a more rigid character of the materials. A small addition of β-glucan to collagen provided a more hydrophilic character. All the materials could swell in in vitro conditions and showed antioxidant activity. Materials do not cause erythrocyte hemolysis. Finely, our cytotoxicity studies indicated that β-glucan can be safely added at small (10% or less) quantity to collagen matrix, they sufficiently support cell growth, and the degradation products of such matrices may actually provide some beneficial effects to the surrounding cells/tissues.
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spelling pubmed-80000142021-03-28 Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing Michalska-Sionkowska, Marta Warżyńska, Oliwia Kaczmarek-Szczepańska, Beata Łukowicz, Krzysztof Osyczka, Anna Maria Walczak, Maciej Materials (Basel) Article Collagen possesses unique properties, e.g., biocompatibility, biodegradability, and non-toxicity. However, collagen material degrades too quickly and has low mechanical properties. One of the methods of polymers’ modification is mixing them to obtain blends. In this study, the influence of β-glucan for collagen material was analyzed. The interaction between the functional groups of the polymer was analyzed by ATR-FTIR (attenuated total reflection-fourier transform infrared) spectroscopy. The influence of β-glucan on mechanical properties was evaluated. The surface properties of materials were assessed using contact angle measurements and the topography of materials was evaluated by AFM (atomic force microscope). The structure of materials was analyzed according to SEM (scanning electron microscopy) pictures. Moreover, the DPPH-free radicals’ scavenging ability and biocompatibility against erythrocytes and HaCaT cells were evaluated. Collagen and β-glucan were bound together by a hydrogen bond. β-glucan addition increased the roughness of the surface of the film and resulted in a more rigid character of the materials. A small addition of β-glucan to collagen provided a more hydrophilic character. All the materials could swell in in vitro conditions and showed antioxidant activity. Materials do not cause erythrocyte hemolysis. Finely, our cytotoxicity studies indicated that β-glucan can be safely added at small (10% or less) quantity to collagen matrix, they sufficiently support cell growth, and the degradation products of such matrices may actually provide some beneficial effects to the surrounding cells/tissues. MDPI 2021-03-10 /pmc/articles/PMC8000014/ /pubmed/33801809 http://dx.doi.org/10.3390/ma14061322 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Michalska-Sionkowska, Marta
Warżyńska, Oliwia
Kaczmarek-Szczepańska, Beata
Łukowicz, Krzysztof
Osyczka, Anna Maria
Walczak, Maciej
Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing
title Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing
title_full Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing
title_fullStr Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing
title_full_unstemmed Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing
title_short Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing
title_sort preparation and characterization of fish skin collagen material modified with β-glucan as potential wound dressing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000014/
https://www.ncbi.nlm.nih.gov/pubmed/33801809
http://dx.doi.org/10.3390/ma14061322
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