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Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity
Scope: House dust mite (HDM) induces Th2 responses in lungs and skin, but its effects in the intestine are poorly known. We aimed to study the involvement of HDM in the initial events that would promote sensitization through the oral route and eventually lead to allergy development. Methods and resu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000190/ https://www.ncbi.nlm.nih.gov/pubmed/33803079 http://dx.doi.org/10.3390/foods10030561 |
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author | Benedé, Sara Pérez-Rodríguez, Leticia Martínez-Blanco, Mónica Molina, Elena López-Fandiño, Rosina |
author_facet | Benedé, Sara Pérez-Rodríguez, Leticia Martínez-Blanco, Mónica Molina, Elena López-Fandiño, Rosina |
author_sort | Benedé, Sara |
collection | PubMed |
description | Scope: House dust mite (HDM) induces Th2 responses in lungs and skin, but its effects in the intestine are poorly known. We aimed to study the involvement of HDM in the initial events that would promote sensitization through the oral route and eventually lead to allergy development. Methods and results: BALB/c mice were exposed intragastrically to proteolytically active and inactive HDM, as such, or in combination with egg white (EW), and inflammatory and type 2 responses were evaluated. Oral administration of HDM, by virtue of its proteolytic activity, promoted the expression, in the small intestine, of genes encoding tight junction proteins, proinflammatory and Th2-biasing cytokines, and it caused expansion of group 2 innate immune cells, upregulation of Th2 cytokines, and dendritic cell migration and activation. In lymphoid tissues, its proteolytically inactivated counterpart also exerted an influence on the expression of surface DC molecules involved in interactions with T cells and in Th2 cell differentiation, which was confirmed in in vitro experiments. However, in our experimental setting we did not find evidence for the promotion of sensitization to coadministered EW. Conclusion: Orally administered HDM upregulates tissue damage factors and also acts as an activator of innate immune cells behaving similarly to potent oral Th2 adjuvants. |
format | Online Article Text |
id | pubmed-8000190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80001902021-03-28 Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity Benedé, Sara Pérez-Rodríguez, Leticia Martínez-Blanco, Mónica Molina, Elena López-Fandiño, Rosina Foods Article Scope: House dust mite (HDM) induces Th2 responses in lungs and skin, but its effects in the intestine are poorly known. We aimed to study the involvement of HDM in the initial events that would promote sensitization through the oral route and eventually lead to allergy development. Methods and results: BALB/c mice were exposed intragastrically to proteolytically active and inactive HDM, as such, or in combination with egg white (EW), and inflammatory and type 2 responses were evaluated. Oral administration of HDM, by virtue of its proteolytic activity, promoted the expression, in the small intestine, of genes encoding tight junction proteins, proinflammatory and Th2-biasing cytokines, and it caused expansion of group 2 innate immune cells, upregulation of Th2 cytokines, and dendritic cell migration and activation. In lymphoid tissues, its proteolytically inactivated counterpart also exerted an influence on the expression of surface DC molecules involved in interactions with T cells and in Th2 cell differentiation, which was confirmed in in vitro experiments. However, in our experimental setting we did not find evidence for the promotion of sensitization to coadministered EW. Conclusion: Orally administered HDM upregulates tissue damage factors and also acts as an activator of innate immune cells behaving similarly to potent oral Th2 adjuvants. MDPI 2021-03-09 /pmc/articles/PMC8000190/ /pubmed/33803079 http://dx.doi.org/10.3390/foods10030561 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Benedé, Sara Pérez-Rodríguez, Leticia Martínez-Blanco, Mónica Molina, Elena López-Fandiño, Rosina Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
title | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
title_full | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
title_fullStr | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
title_full_unstemmed | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
title_short | Oral Exposure to House Dust Mite Activates Intestinal Innate Immunity |
title_sort | oral exposure to house dust mite activates intestinal innate immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000190/ https://www.ncbi.nlm.nih.gov/pubmed/33803079 http://dx.doi.org/10.3390/foods10030561 |
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