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The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

Climate change, environmental pollution and pathogen resistance to available chemical agents are part of the problems that the food industry has to face in order to ensure healthy food for people and livestock. One of the promising solutions to these problems is the use of cold atmospheric pressure...

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Autores principales: Peťková, Mária, Švubová, Renáta, Kyzek, Stanislav, Medvecká, Veronika, Slováková, Ľudmila, Ševčovičová, Andrea, Gálová, Eliška
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000243/
https://www.ncbi.nlm.nih.gov/pubmed/33799521
http://dx.doi.org/10.3390/ijms22062833
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author Peťková, Mária
Švubová, Renáta
Kyzek, Stanislav
Medvecká, Veronika
Slováková, Ľudmila
Ševčovičová, Andrea
Gálová, Eliška
author_facet Peťková, Mária
Švubová, Renáta
Kyzek, Stanislav
Medvecká, Veronika
Slováková, Ľudmila
Ševčovičová, Andrea
Gálová, Eliška
author_sort Peťková, Mária
collection PubMed
description Climate change, environmental pollution and pathogen resistance to available chemical agents are part of the problems that the food industry has to face in order to ensure healthy food for people and livestock. One of the promising solutions to these problems is the use of cold atmospheric pressure plasma (CAPP). Plasma is suitable for efficient surface decontamination of seeds and food products, germination enhancement and obtaining higher yields in agricultural production. However, the plasma effects vary due to plasma source, treatment conditions and seed type. In our study, we tried to find the proper conditions for treatment of barley grains by diffuse coplanar surface barrier discharge, in which positive effects of CAPP, such as enhanced germination or decontamination effects, would be maximized and harmful effects, such as oxidation and genotoxic potential, minimized. Besides germination parameters, we evaluated DNA damage and activities of various germination and antioxidant enzymes in barley seedlings. Plasma exposure resulted in changes in germination parameters and enzyme activities. Longer exposures had also genotoxic effects. As such, our findings indicate that appropriate plasma exposure conditions need to be carefully optimized in order to preserve germination, oxidation balance and genome stability, should CAPP be used in agricultural practice.
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spelling pubmed-80002432021-03-28 The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley Peťková, Mária Švubová, Renáta Kyzek, Stanislav Medvecká, Veronika Slováková, Ľudmila Ševčovičová, Andrea Gálová, Eliška Int J Mol Sci Article Climate change, environmental pollution and pathogen resistance to available chemical agents are part of the problems that the food industry has to face in order to ensure healthy food for people and livestock. One of the promising solutions to these problems is the use of cold atmospheric pressure plasma (CAPP). Plasma is suitable for efficient surface decontamination of seeds and food products, germination enhancement and obtaining higher yields in agricultural production. However, the plasma effects vary due to plasma source, treatment conditions and seed type. In our study, we tried to find the proper conditions for treatment of barley grains by diffuse coplanar surface barrier discharge, in which positive effects of CAPP, such as enhanced germination or decontamination effects, would be maximized and harmful effects, such as oxidation and genotoxic potential, minimized. Besides germination parameters, we evaluated DNA damage and activities of various germination and antioxidant enzymes in barley seedlings. Plasma exposure resulted in changes in germination parameters and enzyme activities. Longer exposures had also genotoxic effects. As such, our findings indicate that appropriate plasma exposure conditions need to be carefully optimized in order to preserve germination, oxidation balance and genome stability, should CAPP be used in agricultural practice. MDPI 2021-03-11 /pmc/articles/PMC8000243/ /pubmed/33799521 http://dx.doi.org/10.3390/ijms22062833 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peťková, Mária
Švubová, Renáta
Kyzek, Stanislav
Medvecká, Veronika
Slováková, Ľudmila
Ševčovičová, Andrea
Gálová, Eliška
The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley
title The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley
title_full The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley
title_fullStr The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley
title_full_unstemmed The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley
title_short The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley
title_sort effects of cold atmospheric pressure plasma on germination parameters, enzyme activities and induction of dna damage in barley
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000243/
https://www.ncbi.nlm.nih.gov/pubmed/33799521
http://dx.doi.org/10.3390/ijms22062833
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