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Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity

Background. Despite considerable interest in the search for a spinal cord injury (SCI) therapy, there is a critical need to develop a panel of diagnostic biomarkers to determine injury severity. In this regard, there is a requirement for continuing research into the fundamental processes of neuroinf...

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Autores principales: Ogurcov, Sergei, Shulman, Iliya, Garanina, Ekaterina, Sabirov, Davran, Baichurina, Irina, Kuznetcov, Maxim, Masgutova, Galina, Kostennikov, Alexander, Rizvanov, Albert, James, Victoria, Mukhamedshina, Yana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000354/
https://www.ncbi.nlm.nih.gov/pubmed/33806460
http://dx.doi.org/10.3390/brainsci11030322
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author Ogurcov, Sergei
Shulman, Iliya
Garanina, Ekaterina
Sabirov, Davran
Baichurina, Irina
Kuznetcov, Maxim
Masgutova, Galina
Kostennikov, Alexander
Rizvanov, Albert
James, Victoria
Mukhamedshina, Yana
author_facet Ogurcov, Sergei
Shulman, Iliya
Garanina, Ekaterina
Sabirov, Davran
Baichurina, Irina
Kuznetcov, Maxim
Masgutova, Galina
Kostennikov, Alexander
Rizvanov, Albert
James, Victoria
Mukhamedshina, Yana
author_sort Ogurcov, Sergei
collection PubMed
description Background. Despite considerable interest in the search for a spinal cord injury (SCI) therapy, there is a critical need to develop a panel of diagnostic biomarkers to determine injury severity. In this regard, there is a requirement for continuing research into the fundamental processes of neuroinflammatory and autoimmune reactions in SCI, identifying changes in the expression of cytokines. Methods. In this pilot study, an extended multiplex analysis of the cytokine profiles in the serum of patients at 2 weeks post-SCI (n = 28) was carried out, together with an additional assessment of neuron-specific enolase (NSE) and vascular endothelial growth factor (VEGF) levels by enzyme-linked immunosorbent assay. A total of 16 uninjured subjects were enrolled as controls. Results. The data obtained showed a large elevation of IFNγ (>52 fold), CCL27 (>13 fold), and CCL26 (>8 fold) 2 weeks after SCI. The levels of cytokines CXCL5, CCL11, CXCL11, IL10, TNFα, and MIF were different between patients with baseline American Spinal Injury Association Impairment Scale (AIS) grades of A or B, whilst IL2 (>2 fold) and MIP-3a (>6 fold) were significantly expressed in the cervical and thoracic regions. There was a trend towards increasing levels of NSE. However, the difference in NSE was lost when the patient set was segregated based on AIS group. Conclusions. Our pilot research demonstrates that serum concentrations of cytokines can be used as an affordable and rapid detection tool to accurately stratify SCI severity in patients.
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spelling pubmed-80003542021-03-28 Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity Ogurcov, Sergei Shulman, Iliya Garanina, Ekaterina Sabirov, Davran Baichurina, Irina Kuznetcov, Maxim Masgutova, Galina Kostennikov, Alexander Rizvanov, Albert James, Victoria Mukhamedshina, Yana Brain Sci Article Background. Despite considerable interest in the search for a spinal cord injury (SCI) therapy, there is a critical need to develop a panel of diagnostic biomarkers to determine injury severity. In this regard, there is a requirement for continuing research into the fundamental processes of neuroinflammatory and autoimmune reactions in SCI, identifying changes in the expression of cytokines. Methods. In this pilot study, an extended multiplex analysis of the cytokine profiles in the serum of patients at 2 weeks post-SCI (n = 28) was carried out, together with an additional assessment of neuron-specific enolase (NSE) and vascular endothelial growth factor (VEGF) levels by enzyme-linked immunosorbent assay. A total of 16 uninjured subjects were enrolled as controls. Results. The data obtained showed a large elevation of IFNγ (>52 fold), CCL27 (>13 fold), and CCL26 (>8 fold) 2 weeks after SCI. The levels of cytokines CXCL5, CCL11, CXCL11, IL10, TNFα, and MIF were different between patients with baseline American Spinal Injury Association Impairment Scale (AIS) grades of A or B, whilst IL2 (>2 fold) and MIP-3a (>6 fold) were significantly expressed in the cervical and thoracic regions. There was a trend towards increasing levels of NSE. However, the difference in NSE was lost when the patient set was segregated based on AIS group. Conclusions. Our pilot research demonstrates that serum concentrations of cytokines can be used as an affordable and rapid detection tool to accurately stratify SCI severity in patients. MDPI 2021-03-04 /pmc/articles/PMC8000354/ /pubmed/33806460 http://dx.doi.org/10.3390/brainsci11030322 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Ogurcov, Sergei
Shulman, Iliya
Garanina, Ekaterina
Sabirov, Davran
Baichurina, Irina
Kuznetcov, Maxim
Masgutova, Galina
Kostennikov, Alexander
Rizvanov, Albert
James, Victoria
Mukhamedshina, Yana
Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity
title Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity
title_full Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity
title_fullStr Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity
title_full_unstemmed Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity
title_short Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity
title_sort blood serum cytokines in patients with subacute spinal cord injury: a pilot study to search for biomarkers of injury severity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000354/
https://www.ncbi.nlm.nih.gov/pubmed/33806460
http://dx.doi.org/10.3390/brainsci11030322
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