Cargando…

Experimental Inoculation of Young Calves with SARS-CoV-2

The host range of SARS-CoV-2 and the susceptibility of animal species to the virus are topics of great interest to the international scientific community. The angiotensin I converting enzyme 2 (ACE2) protein is the major receptor for the virus, and sequence and structural analysis of the protein has...

Descripción completa

Detalles Bibliográficos
Autores principales: Falkenberg, Shollie, Buckley, Alexandra, Laverack, Melissa, Martins, Mathias, Palmer, Mitchell V., Lager, Kelly, Diel, Diego G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000368/
https://www.ncbi.nlm.nih.gov/pubmed/33803455
http://dx.doi.org/10.3390/v13030441
_version_ 1783670984973221888
author Falkenberg, Shollie
Buckley, Alexandra
Laverack, Melissa
Martins, Mathias
Palmer, Mitchell V.
Lager, Kelly
Diel, Diego G.
author_facet Falkenberg, Shollie
Buckley, Alexandra
Laverack, Melissa
Martins, Mathias
Palmer, Mitchell V.
Lager, Kelly
Diel, Diego G.
author_sort Falkenberg, Shollie
collection PubMed
description The host range of SARS-CoV-2 and the susceptibility of animal species to the virus are topics of great interest to the international scientific community. The angiotensin I converting enzyme 2 (ACE2) protein is the major receptor for the virus, and sequence and structural analysis of the protein has been performed to determine its cross-species conservation. Based on these analyses, cattle have been implicated as a potential susceptible species to SARS-CoV-2 and have been reported to have increased ACE2 receptor distribution in the liver and kidney, and lower levels in the lungs. The goal of the current study was to determine the susceptibility of cattle to SARS-CoV-2 utilizing inoculation routes that facilitated exposure to tissues with increased ACE2 receptor distribution. For this, colostrum-deprived calves approximately 6 weeks of age were inoculated via the intratracheal or intravenous routes. Nasal and rectal swab samples, as well as blood and urine samples, were collected over the course of the study to evaluate viral shedding, viremia, and seroconversion. Pyrexia was used as the primary criteria for euthanasia and tissue samples were collected during necropsy. Importantly, SARS-CoV-2 RNA was detected in only two nasal swab samples collected on days 3 and 10 post-inoculation (pi) in two calves; one calf in the intratracheal group and the other calf in the intravenous group, respectively. Additionally, the calf in the intratracheal group that was positive on the nasal swab on day 3 pi also had a positive tracheobronchial lymph node on day 9 pi. Viral nucleic acid load on these samples, based on PCR cycle threshold values, were low and infectious virus was not recovered from the samples. These results suggest that there was no productive replication of SARS-CoV-2 in calves following intratracheal and intravenous inoculation.
format Online
Article
Text
id pubmed-8000368
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80003682021-03-28 Experimental Inoculation of Young Calves with SARS-CoV-2 Falkenberg, Shollie Buckley, Alexandra Laverack, Melissa Martins, Mathias Palmer, Mitchell V. Lager, Kelly Diel, Diego G. Viruses Article The host range of SARS-CoV-2 and the susceptibility of animal species to the virus are topics of great interest to the international scientific community. The angiotensin I converting enzyme 2 (ACE2) protein is the major receptor for the virus, and sequence and structural analysis of the protein has been performed to determine its cross-species conservation. Based on these analyses, cattle have been implicated as a potential susceptible species to SARS-CoV-2 and have been reported to have increased ACE2 receptor distribution in the liver and kidney, and lower levels in the lungs. The goal of the current study was to determine the susceptibility of cattle to SARS-CoV-2 utilizing inoculation routes that facilitated exposure to tissues with increased ACE2 receptor distribution. For this, colostrum-deprived calves approximately 6 weeks of age were inoculated via the intratracheal or intravenous routes. Nasal and rectal swab samples, as well as blood and urine samples, were collected over the course of the study to evaluate viral shedding, viremia, and seroconversion. Pyrexia was used as the primary criteria for euthanasia and tissue samples were collected during necropsy. Importantly, SARS-CoV-2 RNA was detected in only two nasal swab samples collected on days 3 and 10 post-inoculation (pi) in two calves; one calf in the intratracheal group and the other calf in the intravenous group, respectively. Additionally, the calf in the intratracheal group that was positive on the nasal swab on day 3 pi also had a positive tracheobronchial lymph node on day 9 pi. Viral nucleic acid load on these samples, based on PCR cycle threshold values, were low and infectious virus was not recovered from the samples. These results suggest that there was no productive replication of SARS-CoV-2 in calves following intratracheal and intravenous inoculation. MDPI 2021-03-09 /pmc/articles/PMC8000368/ /pubmed/33803455 http://dx.doi.org/10.3390/v13030441 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Falkenberg, Shollie
Buckley, Alexandra
Laverack, Melissa
Martins, Mathias
Palmer, Mitchell V.
Lager, Kelly
Diel, Diego G.
Experimental Inoculation of Young Calves with SARS-CoV-2
title Experimental Inoculation of Young Calves with SARS-CoV-2
title_full Experimental Inoculation of Young Calves with SARS-CoV-2
title_fullStr Experimental Inoculation of Young Calves with SARS-CoV-2
title_full_unstemmed Experimental Inoculation of Young Calves with SARS-CoV-2
title_short Experimental Inoculation of Young Calves with SARS-CoV-2
title_sort experimental inoculation of young calves with sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000368/
https://www.ncbi.nlm.nih.gov/pubmed/33803455
http://dx.doi.org/10.3390/v13030441
work_keys_str_mv AT falkenbergshollie experimentalinoculationofyoungcalveswithsarscov2
AT buckleyalexandra experimentalinoculationofyoungcalveswithsarscov2
AT laverackmelissa experimentalinoculationofyoungcalveswithsarscov2
AT martinsmathias experimentalinoculationofyoungcalveswithsarscov2
AT palmermitchellv experimentalinoculationofyoungcalveswithsarscov2
AT lagerkelly experimentalinoculationofyoungcalveswithsarscov2
AT dieldiegog experimentalinoculationofyoungcalveswithsarscov2