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Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip
The excessive pesticide residues in cereals, fruit and vegetables is a big threat to human health, and it is necessary to develop a portable, low-cost and high-precision pesticide residue detection scheme to replace the large-scale laboratory testing equipment for rapid detection of pesticide residu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000396/ https://www.ncbi.nlm.nih.gov/pubmed/33803445 http://dx.doi.org/10.3390/mi12030290 |
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author | Xie, Jiaqing Pang, Haoran Sun, Ruqian Wang, Tao Meng, Xiaoyu Zhou, Zhikang |
author_facet | Xie, Jiaqing Pang, Haoran Sun, Ruqian Wang, Tao Meng, Xiaoyu Zhou, Zhikang |
author_sort | Xie, Jiaqing |
collection | PubMed |
description | The excessive pesticide residues in cereals, fruit and vegetables is a big threat to human health, and it is necessary to develop a portable, low-cost and high-precision pesticide residue detection scheme to replace the large-scale laboratory testing equipment for rapid detection of pesticide residues. In this study, a colorimetric device for rapid detection of organophosphorus pesticide residues with high precision based on a microfluidic mixer chip was proposed. The microchannel structure with high mixing efficiency was determined by fluid dynamics simulation, while the corresponding microfluidic mixer chip was designed. The microfluidic mixer chip was prepared by a self-developed liquid crystal display (LCD) mask photo-curing machine. The influence of printing parameters on the accuracy of the prepared chip was investigated. The light source with the optimal wavelength of the device was determined by absorption spectrum measurement, and the relationship between the liquid reservoir depth and detection limit was studied by experiments. The correspondence between pesticide concentration and induced voltage was derived. The minimum detection concentration of the device could reach 0.045 mg·L(−1) and the average detection time was reduced to 60 s. The results provide a theoretical and experimental basis for portable and high-precision detection of pesticide residues. |
format | Online Article Text |
id | pubmed-8000396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80003962021-03-28 Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip Xie, Jiaqing Pang, Haoran Sun, Ruqian Wang, Tao Meng, Xiaoyu Zhou, Zhikang Micromachines (Basel) Article The excessive pesticide residues in cereals, fruit and vegetables is a big threat to human health, and it is necessary to develop a portable, low-cost and high-precision pesticide residue detection scheme to replace the large-scale laboratory testing equipment for rapid detection of pesticide residues. In this study, a colorimetric device for rapid detection of organophosphorus pesticide residues with high precision based on a microfluidic mixer chip was proposed. The microchannel structure with high mixing efficiency was determined by fluid dynamics simulation, while the corresponding microfluidic mixer chip was designed. The microfluidic mixer chip was prepared by a self-developed liquid crystal display (LCD) mask photo-curing machine. The influence of printing parameters on the accuracy of the prepared chip was investigated. The light source with the optimal wavelength of the device was determined by absorption spectrum measurement, and the relationship between the liquid reservoir depth and detection limit was studied by experiments. The correspondence between pesticide concentration and induced voltage was derived. The minimum detection concentration of the device could reach 0.045 mg·L(−1) and the average detection time was reduced to 60 s. The results provide a theoretical and experimental basis for portable and high-precision detection of pesticide residues. MDPI 2021-03-09 /pmc/articles/PMC8000396/ /pubmed/33803445 http://dx.doi.org/10.3390/mi12030290 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Xie, Jiaqing Pang, Haoran Sun, Ruqian Wang, Tao Meng, Xiaoyu Zhou, Zhikang Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip |
title | Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip |
title_full | Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip |
title_fullStr | Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip |
title_full_unstemmed | Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip |
title_short | Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip |
title_sort | development of rapid and high-precision colorimetric device for organophosphorus pesticide detection based on microfluidic mixer chip |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000396/ https://www.ncbi.nlm.nih.gov/pubmed/33803445 http://dx.doi.org/10.3390/mi12030290 |
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