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Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced thro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000596/ https://www.ncbi.nlm.nih.gov/pubmed/33807631 http://dx.doi.org/10.3390/microorganisms9030533 |
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author | Contato, Alex Graça de Oliveira, Tássio Brito Aranha, Guilherme Mauro de Freitas, Emanuelle Neiverth Vici, Ana Claudia Nogueira, Karoline Maria Vieira de Lucas, Rosymar Coutinho Scarcella, Ana Sílvia de Almeida Buckeridge, Marcos Silveira Silva, Roberto Nascimento Polizeli, Maria de Lourdes Teixeira de Moraes |
author_facet | Contato, Alex Graça de Oliveira, Tássio Brito Aranha, Guilherme Mauro de Freitas, Emanuelle Neiverth Vici, Ana Claudia Nogueira, Karoline Maria Vieira de Lucas, Rosymar Coutinho Scarcella, Ana Sílvia de Almeida Buckeridge, Marcos Silveira Silva, Roberto Nascimento Polizeli, Maria de Lourdes Teixeira de Moraes |
author_sort | Contato, Alex Graça |
collection | PubMed |
description | The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors. |
format | Online Article Text |
id | pubmed-8000596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80005962021-03-28 Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse Contato, Alex Graça de Oliveira, Tássio Brito Aranha, Guilherme Mauro de Freitas, Emanuelle Neiverth Vici, Ana Claudia Nogueira, Karoline Maria Vieira de Lucas, Rosymar Coutinho Scarcella, Ana Sílvia de Almeida Buckeridge, Marcos Silveira Silva, Roberto Nascimento Polizeli, Maria de Lourdes Teixeira de Moraes Microorganisms Article The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors. MDPI 2021-03-05 /pmc/articles/PMC8000596/ /pubmed/33807631 http://dx.doi.org/10.3390/microorganisms9030533 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Contato, Alex Graça de Oliveira, Tássio Brito Aranha, Guilherme Mauro de Freitas, Emanuelle Neiverth Vici, Ana Claudia Nogueira, Karoline Maria Vieira de Lucas, Rosymar Coutinho Scarcella, Ana Sílvia de Almeida Buckeridge, Marcos Silveira Silva, Roberto Nascimento Polizeli, Maria de Lourdes Teixeira de Moraes Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse |
title | Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse |
title_full | Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse |
title_fullStr | Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse |
title_full_unstemmed | Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse |
title_short | Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse |
title_sort | prospection of fungal lignocellulolytic enzymes produced from jatoba (hymenaea courbaril) and tamarind (tamarindus indica) seeds: scaling for bioreactor and saccharification profile of sugarcane bagasse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000596/ https://www.ncbi.nlm.nih.gov/pubmed/33807631 http://dx.doi.org/10.3390/microorganisms9030533 |
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