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Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced thro...

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Autores principales: Contato, Alex Graça, de Oliveira, Tássio Brito, Aranha, Guilherme Mauro, de Freitas, Emanuelle Neiverth, Vici, Ana Claudia, Nogueira, Karoline Maria Vieira, de Lucas, Rosymar Coutinho, Scarcella, Ana Sílvia de Almeida, Buckeridge, Marcos Silveira, Silva, Roberto Nascimento, Polizeli, Maria de Lourdes Teixeira de Moraes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000596/
https://www.ncbi.nlm.nih.gov/pubmed/33807631
http://dx.doi.org/10.3390/microorganisms9030533
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author Contato, Alex Graça
de Oliveira, Tássio Brito
Aranha, Guilherme Mauro
de Freitas, Emanuelle Neiverth
Vici, Ana Claudia
Nogueira, Karoline Maria Vieira
de Lucas, Rosymar Coutinho
Scarcella, Ana Sílvia de Almeida
Buckeridge, Marcos Silveira
Silva, Roberto Nascimento
Polizeli, Maria de Lourdes Teixeira de Moraes
author_facet Contato, Alex Graça
de Oliveira, Tássio Brito
Aranha, Guilherme Mauro
de Freitas, Emanuelle Neiverth
Vici, Ana Claudia
Nogueira, Karoline Maria Vieira
de Lucas, Rosymar Coutinho
Scarcella, Ana Sílvia de Almeida
Buckeridge, Marcos Silveira
Silva, Roberto Nascimento
Polizeli, Maria de Lourdes Teixeira de Moraes
author_sort Contato, Alex Graça
collection PubMed
description The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.
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spelling pubmed-80005962021-03-28 Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse Contato, Alex Graça de Oliveira, Tássio Brito Aranha, Guilherme Mauro de Freitas, Emanuelle Neiverth Vici, Ana Claudia Nogueira, Karoline Maria Vieira de Lucas, Rosymar Coutinho Scarcella, Ana Sílvia de Almeida Buckeridge, Marcos Silveira Silva, Roberto Nascimento Polizeli, Maria de Lourdes Teixeira de Moraes Microorganisms Article The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors. MDPI 2021-03-05 /pmc/articles/PMC8000596/ /pubmed/33807631 http://dx.doi.org/10.3390/microorganisms9030533 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Contato, Alex Graça
de Oliveira, Tássio Brito
Aranha, Guilherme Mauro
de Freitas, Emanuelle Neiverth
Vici, Ana Claudia
Nogueira, Karoline Maria Vieira
de Lucas, Rosymar Coutinho
Scarcella, Ana Sílvia de Almeida
Buckeridge, Marcos Silveira
Silva, Roberto Nascimento
Polizeli, Maria de Lourdes Teixeira de Moraes
Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
title Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
title_full Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
title_fullStr Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
title_full_unstemmed Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
title_short Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse
title_sort prospection of fungal lignocellulolytic enzymes produced from jatoba (hymenaea courbaril) and tamarind (tamarindus indica) seeds: scaling for bioreactor and saccharification profile of sugarcane bagasse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000596/
https://www.ncbi.nlm.nih.gov/pubmed/33807631
http://dx.doi.org/10.3390/microorganisms9030533
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