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Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii
Pleurotus eryngii produces various functional molecules that mediate physiological functions in humans. Recently, we observed that P. eryngii produces molecules that have antidepressant functions. An ethanol extract of the fruiting body of P. eryngii was obtained, and the extract was purified by XAD...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000720/ https://www.ncbi.nlm.nih.gov/pubmed/33800437 http://dx.doi.org/10.3390/jof7030190 |
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author | Park, Yong-Sung Jang, Subin Lee, Hyunkoo Kang, Suzie Seo, Hyewon Yeon, Seoyeong Lee, Dongho Yun, Cheol-Won |
author_facet | Park, Yong-Sung Jang, Subin Lee, Hyunkoo Kang, Suzie Seo, Hyewon Yeon, Seoyeong Lee, Dongho Yun, Cheol-Won |
author_sort | Park, Yong-Sung |
collection | PubMed |
description | Pleurotus eryngii produces various functional molecules that mediate physiological functions in humans. Recently, we observed that P. eryngii produces molecules that have antidepressant functions. An ethanol extract of the fruiting body of P. eryngii was obtained, and the extract was purified by XAD-16 resin using an open column system. The ethanol eluate was separated by HPLC, and the fraction with an antidepressant function was identified. Using LC-MS, the molecular structure of the HPLC fraction with antidepressant function was identified as that of tryptamine, a functional molecule that is a tryptophan derivative. The antidepressant effect was identified from the ethanol extract, XAD-16 column eluate, and HPLC fraction by a serotonin receptor binding assay and a cell-based binding assay. Furthermore, a forced swimming test (FST) showed that the mice treated with purified fractions of P. eryngii exhibited decreased immobility time compared with nontreated mice. From these results, we suggest that the extract of P. eryngii has an antidepressant function and that it may be employed as an antidepressant health supplement. |
format | Online Article Text |
id | pubmed-8000720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80007202021-03-28 Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii Park, Yong-Sung Jang, Subin Lee, Hyunkoo Kang, Suzie Seo, Hyewon Yeon, Seoyeong Lee, Dongho Yun, Cheol-Won J Fungi (Basel) Article Pleurotus eryngii produces various functional molecules that mediate physiological functions in humans. Recently, we observed that P. eryngii produces molecules that have antidepressant functions. An ethanol extract of the fruiting body of P. eryngii was obtained, and the extract was purified by XAD-16 resin using an open column system. The ethanol eluate was separated by HPLC, and the fraction with an antidepressant function was identified. Using LC-MS, the molecular structure of the HPLC fraction with antidepressant function was identified as that of tryptamine, a functional molecule that is a tryptophan derivative. The antidepressant effect was identified from the ethanol extract, XAD-16 column eluate, and HPLC fraction by a serotonin receptor binding assay and a cell-based binding assay. Furthermore, a forced swimming test (FST) showed that the mice treated with purified fractions of P. eryngii exhibited decreased immobility time compared with nontreated mice. From these results, we suggest that the extract of P. eryngii has an antidepressant function and that it may be employed as an antidepressant health supplement. MDPI 2021-03-08 /pmc/articles/PMC8000720/ /pubmed/33800437 http://dx.doi.org/10.3390/jof7030190 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Park, Yong-Sung Jang, Subin Lee, Hyunkoo Kang, Suzie Seo, Hyewon Yeon, Seoyeong Lee, Dongho Yun, Cheol-Won Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii |
title | Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii |
title_full | Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii |
title_fullStr | Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii |
title_full_unstemmed | Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii |
title_short | Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii |
title_sort | identification of the antidepressant function of the edible mushroom pleurotus eryngii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000720/ https://www.ncbi.nlm.nih.gov/pubmed/33800437 http://dx.doi.org/10.3390/jof7030190 |
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