Cargando…
Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases
This article demonstrates the possibility to use a novel powerful approach based on Raman mapping of analyte solutions drop casted on a disordered array of Ag covered silicon nanowires (Ag/SiNWs), to identify the characteristic spectral signal of the four DNA bases, adenine (A), thymine (T), cytosin...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000830/ https://www.ncbi.nlm.nih.gov/pubmed/33806524 http://dx.doi.org/10.3390/mi12030264 |
_version_ | 1783671087894102016 |
---|---|
author | Mussi, Valentina Ledda, Mario Convertino, Annalisa Lisi, Antonella |
author_facet | Mussi, Valentina Ledda, Mario Convertino, Annalisa Lisi, Antonella |
author_sort | Mussi, Valentina |
collection | PubMed |
description | This article demonstrates the possibility to use a novel powerful approach based on Raman mapping of analyte solutions drop casted on a disordered array of Ag covered silicon nanowires (Ag/SiNWs), to identify the characteristic spectral signal of the four DNA bases, adenine (A), thymine (T), cytosine (C), and guanine (G), at concentration as low as 10 ng/µL, and to study their specific way of interacting with the nanostructured substrate. The results show a distinctive and amplified interaction of guanine, the base that is most susceptible to oxidation, with the nanostructured surface. Our findings explain the recently revealed diverse behaviour of cancer and normal DNA deposited on the same Ag/SiNWs, which is ascribed to mechanical deformation and base lesions present on the oxidised DNA molecule backbone and causes detectable variation in the Raman signal, usable for diagnostic purposes. The notable bio-analytical capability of the presented platform, and its sensitivity to the molecule mechanical conformation at the single-base level, thus provides a new reliable, rapid, label-free DNA diagnostic methodology alternative to more sophisticated and expensive sequencing ones. |
format | Online Article Text |
id | pubmed-8000830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80008302021-03-28 Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases Mussi, Valentina Ledda, Mario Convertino, Annalisa Lisi, Antonella Micromachines (Basel) Article This article demonstrates the possibility to use a novel powerful approach based on Raman mapping of analyte solutions drop casted on a disordered array of Ag covered silicon nanowires (Ag/SiNWs), to identify the characteristic spectral signal of the four DNA bases, adenine (A), thymine (T), cytosine (C), and guanine (G), at concentration as low as 10 ng/µL, and to study their specific way of interacting with the nanostructured substrate. The results show a distinctive and amplified interaction of guanine, the base that is most susceptible to oxidation, with the nanostructured surface. Our findings explain the recently revealed diverse behaviour of cancer and normal DNA deposited on the same Ag/SiNWs, which is ascribed to mechanical deformation and base lesions present on the oxidised DNA molecule backbone and causes detectable variation in the Raman signal, usable for diagnostic purposes. The notable bio-analytical capability of the presented platform, and its sensitivity to the molecule mechanical conformation at the single-base level, thus provides a new reliable, rapid, label-free DNA diagnostic methodology alternative to more sophisticated and expensive sequencing ones. MDPI 2021-03-04 /pmc/articles/PMC8000830/ /pubmed/33806524 http://dx.doi.org/10.3390/mi12030264 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Mussi, Valentina Ledda, Mario Convertino, Annalisa Lisi, Antonella Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases |
title | Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases |
title_full | Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases |
title_fullStr | Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases |
title_full_unstemmed | Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases |
title_short | Raman Mapping of Biological Systems Interacting with a Disordered Nanostructured Surface: A Simple and Powerful Approach to the Label-Free Analysis of Single DNA Bases |
title_sort | raman mapping of biological systems interacting with a disordered nanostructured surface: a simple and powerful approach to the label-free analysis of single dna bases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000830/ https://www.ncbi.nlm.nih.gov/pubmed/33806524 http://dx.doi.org/10.3390/mi12030264 |
work_keys_str_mv | AT mussivalentina ramanmappingofbiologicalsystemsinteractingwithadisorderednanostructuredsurfaceasimpleandpowerfulapproachtothelabelfreeanalysisofsinglednabases AT leddamario ramanmappingofbiologicalsystemsinteractingwithadisorderednanostructuredsurfaceasimpleandpowerfulapproachtothelabelfreeanalysisofsinglednabases AT convertinoannalisa ramanmappingofbiologicalsystemsinteractingwithadisorderednanostructuredsurfaceasimpleandpowerfulapproachtothelabelfreeanalysisofsinglednabases AT lisiantonella ramanmappingofbiologicalsystemsinteractingwithadisorderednanostructuredsurfaceasimpleandpowerfulapproachtothelabelfreeanalysisofsinglednabases |