Cargando…
Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation
Possessing a variety of medicinal functions, Olea europaea L. is widely cultivated across the world. However, the anti-inflammatory mechanism of Olea europaea is not yet fully elucidated. In this study, how the methanol extract of the leaves of Olea europaea (Oe-ME) can suppress in vitro inflammator...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000943/ https://www.ncbi.nlm.nih.gov/pubmed/33799767 http://dx.doi.org/10.3390/molecules26061540 |
_version_ | 1783671114431463424 |
---|---|
author | Song, Chaoran Kim, Mi-Yeon Cho, Jae Youl |
author_facet | Song, Chaoran Kim, Mi-Yeon Cho, Jae Youl |
author_sort | Song, Chaoran |
collection | PubMed |
description | Possessing a variety of medicinal functions, Olea europaea L. is widely cultivated across the world. However, the anti-inflammatory mechanism of Olea europaea is not yet fully elucidated. In this study, how the methanol extract of the leaves of Olea europaea (Oe-ME) can suppress in vitro inflammatory responses was examined in terms of the identification of the target protein. RAW264.7 and HEK293T cells were used to study macrophage-mediated inflammatory responses and to validate the target protein using PCR, immunoblotting, nuclear fraction, overexpression, and cellular thermal shift assay (CETSA) under fixed conditions. Oe-ME treatment inhibited the mRNA expression levels of cyclooxygenase (COX)-2, matrix metallopeptidase (MMP)-9, and intercellular adhesion molecule-1 (ICAM-1) in activated RAW264.7 cells. Oe-ME diminished the activation of activator protein (AP)-1 and the phosphorylation of its upstream signaling cascades, including extracellular signal regulated kinase (ERK), mitogen-activated protein kinase kinase 1/2 (MEK1/2), c-Jun N-terminal kinase (JNK), mitogen-activated protein kinase kinase 3/6 (MKK3/6), p38, MKK7, and transforming growth factor-β-activated kinase 1 (TAK1), in stimulated-RAW264.7 cells. Overexpression and CETSA were carried out to verify that TAK1 is the target of Oe-ME. Our results suggest that the anti-inflammatory effect of Oe-ME could be attributed to its control of posttranslational modification and transcription of TAK1. |
format | Online Article Text |
id | pubmed-8000943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80009432021-03-28 Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation Song, Chaoran Kim, Mi-Yeon Cho, Jae Youl Molecules Article Possessing a variety of medicinal functions, Olea europaea L. is widely cultivated across the world. However, the anti-inflammatory mechanism of Olea europaea is not yet fully elucidated. In this study, how the methanol extract of the leaves of Olea europaea (Oe-ME) can suppress in vitro inflammatory responses was examined in terms of the identification of the target protein. RAW264.7 and HEK293T cells were used to study macrophage-mediated inflammatory responses and to validate the target protein using PCR, immunoblotting, nuclear fraction, overexpression, and cellular thermal shift assay (CETSA) under fixed conditions. Oe-ME treatment inhibited the mRNA expression levels of cyclooxygenase (COX)-2, matrix metallopeptidase (MMP)-9, and intercellular adhesion molecule-1 (ICAM-1) in activated RAW264.7 cells. Oe-ME diminished the activation of activator protein (AP)-1 and the phosphorylation of its upstream signaling cascades, including extracellular signal regulated kinase (ERK), mitogen-activated protein kinase kinase 1/2 (MEK1/2), c-Jun N-terminal kinase (JNK), mitogen-activated protein kinase kinase 3/6 (MKK3/6), p38, MKK7, and transforming growth factor-β-activated kinase 1 (TAK1), in stimulated-RAW264.7 cells. Overexpression and CETSA were carried out to verify that TAK1 is the target of Oe-ME. Our results suggest that the anti-inflammatory effect of Oe-ME could be attributed to its control of posttranslational modification and transcription of TAK1. MDPI 2021-03-11 /pmc/articles/PMC8000943/ /pubmed/33799767 http://dx.doi.org/10.3390/molecules26061540 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Song, Chaoran Kim, Mi-Yeon Cho, Jae Youl Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation |
title | Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation |
title_full | Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation |
title_fullStr | Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation |
title_full_unstemmed | Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation |
title_short | Olea europaea Suppresses Inflammation by Targeting TAK1-Mediated MAP Kinase Activation |
title_sort | olea europaea suppresses inflammation by targeting tak1-mediated map kinase activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000943/ https://www.ncbi.nlm.nih.gov/pubmed/33799767 http://dx.doi.org/10.3390/molecules26061540 |
work_keys_str_mv | AT songchaoran oleaeuropaeasuppressesinflammationbytargetingtak1mediatedmapkinaseactivation AT kimmiyeon oleaeuropaeasuppressesinflammationbytargetingtak1mediatedmapkinaseactivation AT chojaeyoul oleaeuropaeasuppressesinflammationbytargetingtak1mediatedmapkinaseactivation |