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An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples

This study provides design of a low-cost and open source add-on device that enhances the functionality of the popular EVOM(®) instrument for transepithelial/endothelial electrical resistance (TEER) measurement. The original EVOM(®) instrument is designed for measuring TEER in transwell samples manua...

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Detalles Bibliográficos
Autores principales: Raut, Bibek, Chen, Li-Jiun, Hori, Takeshi, Kaji, Hirokazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000980/
https://www.ncbi.nlm.nih.gov/pubmed/33800233
http://dx.doi.org/10.3390/mi12030282
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author Raut, Bibek
Chen, Li-Jiun
Hori, Takeshi
Kaji, Hirokazu
author_facet Raut, Bibek
Chen, Li-Jiun
Hori, Takeshi
Kaji, Hirokazu
author_sort Raut, Bibek
collection PubMed
description This study provides design of a low-cost and open source add-on device that enhances the functionality of the popular EVOM(®) instrument for transepithelial/endothelial electrical resistance (TEER) measurement. The original EVOM(®) instrument is designed for measuring TEER in transwell samples manually using a pair of Ag/AgCl electrodes. The inconsistency in electrode placement, temperature variation, and a typically large (12–24 h) time interval between measurements result in large data variabilities. Thus, to solve the current limitation of the EVOM(®) instrument, we built an add-on device using a custom designed electronic board and a 3D printed electrode holder that allowed automated TEER measurements in multiple transwell samples. To demonstrate the functionality of the device prototype, we monitored TEER in 4 transwell samples containing retinal cells (ARPE-19) for 67 h. Furthermore, by monitoring temperature of the cell culture medium, we were able to detect fluctuations in TEER due to temperature change after the medium change process, and were able to correct the data offset. Although we demonstrated the use of our add-on device on EVOM(®) instrument only, the concept (multiplexing using digitally controlled relays) and hardware (custom data logger) presented here can be applied to more advanced TEER instruments to improve the performance of those devices.
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spelling pubmed-80009802021-03-28 An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples Raut, Bibek Chen, Li-Jiun Hori, Takeshi Kaji, Hirokazu Micromachines (Basel) Article This study provides design of a low-cost and open source add-on device that enhances the functionality of the popular EVOM(®) instrument for transepithelial/endothelial electrical resistance (TEER) measurement. The original EVOM(®) instrument is designed for measuring TEER in transwell samples manually using a pair of Ag/AgCl electrodes. The inconsistency in electrode placement, temperature variation, and a typically large (12–24 h) time interval between measurements result in large data variabilities. Thus, to solve the current limitation of the EVOM(®) instrument, we built an add-on device using a custom designed electronic board and a 3D printed electrode holder that allowed automated TEER measurements in multiple transwell samples. To demonstrate the functionality of the device prototype, we monitored TEER in 4 transwell samples containing retinal cells (ARPE-19) for 67 h. Furthermore, by monitoring temperature of the cell culture medium, we were able to detect fluctuations in TEER due to temperature change after the medium change process, and were able to correct the data offset. Although we demonstrated the use of our add-on device on EVOM(®) instrument only, the concept (multiplexing using digitally controlled relays) and hardware (custom data logger) presented here can be applied to more advanced TEER instruments to improve the performance of those devices. MDPI 2021-03-08 /pmc/articles/PMC8000980/ /pubmed/33800233 http://dx.doi.org/10.3390/mi12030282 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Raut, Bibek
Chen, Li-Jiun
Hori, Takeshi
Kaji, Hirokazu
An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples
title An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples
title_full An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples
title_fullStr An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples
title_full_unstemmed An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples
title_short An Open-Source Add-On EVOM(®) Device for Real-Time Transepithelial/Endothelial Electrical Resistance Measurements in Multiple Transwell Samples
title_sort open-source add-on evom(®) device for real-time transepithelial/endothelial electrical resistance measurements in multiple transwell samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000980/
https://www.ncbi.nlm.nih.gov/pubmed/33800233
http://dx.doi.org/10.3390/mi12030282
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