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Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens

A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing t...

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Autores principales: Kim, Ah-Young, Kim, Hyejin, Park, Sun Young, Park, Sang Hyun, Kim, Jae-Seok, Park, Jung-Won, Park, Jong-Hyeon, Ko, Young-Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001202/
https://www.ncbi.nlm.nih.gov/pubmed/33809252
http://dx.doi.org/10.3390/vaccines9030252
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author Kim, Ah-Young
Kim, Hyejin
Park, Sun Young
Park, Sang Hyun
Kim, Jae-Seok
Park, Jung-Won
Park, Jong-Hyeon
Ko, Young-Joon
author_facet Kim, Ah-Young
Kim, Hyejin
Park, Sun Young
Park, Sang Hyun
Kim, Jae-Seok
Park, Jung-Won
Park, Jong-Hyeon
Ko, Young-Joon
author_sort Kim, Ah-Young
collection PubMed
description A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. In contrast to O JC vaccine antigens stored in routinely used Tris-buffered or phosphate-buffered saline, those stored in Tris-KCl buffer showed extended shelf-life at both 4 °C and −70 °C. Additionally, the combined application of 10% sucrose and 5% lactalbumin hydrolysate could protect O JC 146S particles from massive structural breakdown in an aqueous state for up to one year. The stabilizer composition was also effective for other FMDV strains, including serotypes A and Asia 1. With this stabilizer composition, FMD vaccine antigens could be flexibly preserved during the general production process, pending status under refrigeration and banking under ultrafreezing.
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spelling pubmed-80012022021-03-28 Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens Kim, Ah-Young Kim, Hyejin Park, Sun Young Park, Sang Hyun Kim, Jae-Seok Park, Jung-Won Park, Jong-Hyeon Ko, Young-Joon Vaccines (Basel) Article A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. In contrast to O JC vaccine antigens stored in routinely used Tris-buffered or phosphate-buffered saline, those stored in Tris-KCl buffer showed extended shelf-life at both 4 °C and −70 °C. Additionally, the combined application of 10% sucrose and 5% lactalbumin hydrolysate could protect O JC 146S particles from massive structural breakdown in an aqueous state for up to one year. The stabilizer composition was also effective for other FMDV strains, including serotypes A and Asia 1. With this stabilizer composition, FMD vaccine antigens could be flexibly preserved during the general production process, pending status under refrigeration and banking under ultrafreezing. MDPI 2021-03-12 /pmc/articles/PMC8001202/ /pubmed/33809252 http://dx.doi.org/10.3390/vaccines9030252 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Kim, Ah-Young
Kim, Hyejin
Park, Sun Young
Park, Sang Hyun
Kim, Jae-Seok
Park, Jung-Won
Park, Jong-Hyeon
Ko, Young-Joon
Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
title Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
title_full Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
title_fullStr Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
title_full_unstemmed Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
title_short Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
title_sort development of a potent stabilizer for long-term storage of foot-and-mouth disease vaccine antigens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001202/
https://www.ncbi.nlm.nih.gov/pubmed/33809252
http://dx.doi.org/10.3390/vaccines9030252
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