Cargando…

2LTRZFP Interacts Specifically to HIV-1 DNA without Off-Target Effects as Determined by Biolayer Interferometry

Protein and DNA interactions are crucial for many cellular processes. Biolayer Interferometry (BLI) is a label-free technology for determining kinetic biomolecular interactions with high accuracy results. In the present study, we determined the kinetic binding of a zinc finger scaffold, 2LTRZFP, whi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chupradit, Koollawat, Thongkum, Weeraya, Juntit, On-anong, Sornsuwan, Kanokporn, Tayapiwatana, Chatchai
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001305/ https://www.ncbi.nlm.nih.gov/pubmed/33800287 http://dx.doi.org/10.3390/bios11030076

Ejemplares similares

Specific Interaction of DARPin with HIV-1 CA(NTD) Disturbs the Distribution of Gag, RNA Packaging, and Tetraspanin Remodelling in the Membrane
por: Moonmuang, Sutpirat, et al.
Publicado: (2022)

Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
por: Thongkum, Weeraya, et al.
Publicado: (2021)

Validation of Promoters and Codon Optimization on CRISPR/Cas9-Engineered Jurkat Cells Stably Expressing αRep4E3 for Interfering with HIV-1 Replication
por: Chupradit, Koollawat, et al.
Publicado: (2022)

Dimeric Ankyrin with Inverted Module Promotes Bifunctional Property in Capturing Capsid to Impede HIV-1 Replication
por: Juntit, On-anong, et al.
Publicado: (2023)

Determination of a distinguished interferon gamma epitope recognized by monoclonal antibody relating to autoantibody associated immunodeficiency
por: Yasamut, Umpa, et al.
Publicado: (2022)

Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors
por: Sakkhachornphop, Supachai, et al.
Publicado: (2015)

Immunoreactivity of humanized single-chain variable fragment against its functional epitope on domain 1 of CD147
por: Intasai, Nutjeera, et al.
Publicado: (2022)

Biolayer interferometry for DNA-protein interactions
por: Barrows, John K., et al.
Publicado: (2022)

Occupation of a thermoresistant-scaffold (αRep) at SP1-NC cleavage site disturbs the function of HIV-1 protease
por: Hadpech, Sudarat, et al.
Publicado: (2020)

The Occluded Epitope Residing in Spike Receptor-Binding Motif Is Essential for Cross-Neutralization of SARS-CoV-2 Delta Variant
por: Thongkum, Weeraya, et al.
Publicado: (2022)

Protein-Protein Interactions: Insight from Molecular Dynamics Simulations and Nanoparticle Tracking Analysis
por: Chong, Wei Lim, et al.
Publicado: (2021)

Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production
por: Sornsuwan, Kanokporn, et al.
Publicado: (2021)

Kinetic Titration Series with Biolayer Interferometry
por: Frenzel, Daniel, et al.
Publicado: (2014)

A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors
por: Chupradit, Koollawat, et al.
Publicado: (2022)

Influenza vaccine titer determination using biolayer interferometry (BLI)
por: Wheatley, David W, et al.
Publicado: (2015)

Modulated expression of the HIV-1 2LTR zinc finger efficiently interferes with the HIV integration process
por: Moonmuang, Sutpirat, et al.
Publicado: (2018)

Current Peptide and Protein Candidates Challenging HIV Therapy beyond the Vaccine Era
por: Chupradit, Koollawat, et al.
Publicado: (2017)

Characterization of antibody-antigen interactions using biolayer interferometry
por: Noy-Porat, Tal, et al.
Publicado: (2021)

Measuring the effects of macromolecular crowding on antibody function with biolayer interferometry
por: Kim, Dorothy M., et al.
Publicado: (2019)

Semi-quantification and Potency Verification of the HIV Protease Inhibitor Based on the Matrix-Capsid Protein Immobilized Nickel (II)/NTA-Tol/Graphene Oxide/SPCE Electrochemical Biosensor
por: Thongkum, Weeraya, et al.
Publicado: (2023)

Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding
por: Murali, Shruthi, et al.
Publicado: (2022)

Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry
por: Dzimianski, John V., et al.
Publicado: (2020)

Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry
por: Dzimianski, John V., et al.
Publicado: (2020)

On-Site Biolayer Interferometry-Based Biosensing of Carbamazepine in Whole Blood of Epileptic Patients
por: Bian, Sumin, et al.
Publicado: (2021)

Suppressing Nonspecific Binding in Biolayer Interferometry Experiments for Weak Ligand–Analyte Interactions
por: Dubrow, Alyssa, et al.
Publicado: (2022)

Qualification of a Biolayer Interferometry Assay to Support AZD7442 Resistance Monitoring
por: Brady, Tyler, et al.
Publicado: (2022)

Engineered Zinc Finger Protein Targeting 2LTR Inhibits HIV Integration in Hematopoietic Stem and Progenitor Cell-Derived Macrophages: In Vitro Study
por: Chupradit, Koollawat, et al.
Publicado: (2022)

Potency determination of inactivated H7 influenza vaccines using monoclonal antibody‐based ELISA and biolayer interferometry assays
por: Vasudevan, Anupama, et al.
Publicado: (2017)

Biolayer Interferometry Analysis for a Higher Throughput Quantification of In-Process Samples of a Rotavirus Vaccine
por: Carvalho, Sofia B., et al.
Publicado: (2022)

Evaluating the immunogenicity of heparin and heparin derivatives by measuring their binding to platelet factor 4 using biolayer interferometry
por: Chen, Qingqing, et al.
Publicado: (2022)

Alpha-helicoidal HEAT-like Repeat Proteins (αRep) Selected as Interactors of HIV-1 Nucleocapsid Negatively Interfere with Viral Genome Packaging and Virus Maturation
por: Hadpech, Sudarat, et al.
Publicado: (2017)

Impact of the detection of ζ-globin chains and hemoglobin Bart’s using immunochromatographic strip tests for α(0)-thalassemia (--(SEA)) differential diagnosis
por: Pata, Supansa, et al.
Publicado: (2019)

Dynamic of anti-spike receptor binding domain (RBD) levels and short-term adverse events following a heterologous booster dose of BNT162b2 after two doses of CoronaVac in Thai health care workers
por: Kitro, Amonrphat, et al.
Publicado: (2022)

Qualified Biolayer Interferometry Avidity Measurements Distinguish the Heterogeneity of Antibody Interactions with Plasmodium falciparum Circumsporozoite Protein Antigens
por: Dennison, S. Moses, et al.
Publicado: (2018)

Rapid Biolayer Interferometry Measurements of Urinary CXCL9 to Detect Cellular Infiltrates Noninvasively After Kidney Transplantation
por: Gandolfini, Ilaria, et al.
Publicado: (2017)

Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2
por: Markwalter, C.F., et al.
Publicado: (2017)

Neutralizing Activity of Anti-interferon-γ Autoantibodies in Adult-Onset Immunodeficiency Is Associated With Their Binding Domains
por: Yasamut, Umpa, et al.
Publicado: (2019)

Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust
por: Sanders, Melanie, et al.
Publicado: (2016)

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
por: Machen, Alexandra J., et al.
Publicado: (2018)

Biophysical Characterization of the Oligomeric States of Recombinant Immunoglobulins Type-M and Their C1q-Binding Kinetics by Biolayer Interferometry
por: Chouquet, Anne, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_qcsv6smge6jb40m4dcivqcvduk