Cargando…

Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties

Alpha-linolenic acid (ALA) is widely regarded as the main beneficial component of flax for the prevention of cardiovascular disease. We evaluated the effect of the transgenic flaxseed W86—which is rich in ALA—on the lipid profile, atherosclerosis progression, and vascular reactivity in hypercholeste...

Descripción completa

Detalles Bibliográficos
Autores principales: Bujok, Jolanta, Miśta, Dorota, Wincewicz, Edyta, Króliczewska, Bożena, Dzimira, Stanisław, Żuk, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001360/
https://www.ncbi.nlm.nih.gov/pubmed/33806676
http://dx.doi.org/10.3390/foods10030534
_version_ 1783671211631312896
author Bujok, Jolanta
Miśta, Dorota
Wincewicz, Edyta
Króliczewska, Bożena
Dzimira, Stanisław
Żuk, Magdalena
author_facet Bujok, Jolanta
Miśta, Dorota
Wincewicz, Edyta
Króliczewska, Bożena
Dzimira, Stanisław
Żuk, Magdalena
author_sort Bujok, Jolanta
collection PubMed
description Alpha-linolenic acid (ALA) is widely regarded as the main beneficial component of flax for the prevention of cardiovascular disease. We evaluated the effect of the transgenic flaxseed W86—which is rich in ALA—on the lipid profile, atherosclerosis progression, and vascular reactivity in hypercholesterolemic rabbits compared to the parental cultivar Linola with a very low ALA content. Rabbits were fed a basal diet (control) or a basal diet supplemented with 1% cholesterol, 1% cholesterol and 10% flaxseed W86, or 1% cholesterol and 10% Linola flaxseed. A high-cholesterol diet resulted in an elevated plasma cholesterol and triglyceride levels compared to the control animals. Aortic sections from rabbits fed Linola had lower deposits of foamy cells than those from rabbits fed W86. A potassium-induced and phenylephrine-induced contractile response was enhanced by a high-cholesterol diet and not influenced by the W86 or Linola flaxseed. Pretreatment of the aortic rings with nitro-L-arginine methyl ester resulted in a concentration-dependent tendency to increase the reaction amplitude in the control and high-cholesterol diet groups but not the flaxseed groups. Linola flaxseed with a low ALA content more effectively reduced the atherosclerosis progression compared with the W86 flaxseed with a high concentration of stable ALA. Aorta contractility studies suggested that flaxseed ameliorated an increased contractility in hypercholesterolemia but had little or no impact on NO synthesis in the vascular wall.
format Online
Article
Text
id pubmed-8001360
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80013602021-03-28 Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties Bujok, Jolanta Miśta, Dorota Wincewicz, Edyta Króliczewska, Bożena Dzimira, Stanisław Żuk, Magdalena Foods Article Alpha-linolenic acid (ALA) is widely regarded as the main beneficial component of flax for the prevention of cardiovascular disease. We evaluated the effect of the transgenic flaxseed W86—which is rich in ALA—on the lipid profile, atherosclerosis progression, and vascular reactivity in hypercholesterolemic rabbits compared to the parental cultivar Linola with a very low ALA content. Rabbits were fed a basal diet (control) or a basal diet supplemented with 1% cholesterol, 1% cholesterol and 10% flaxseed W86, or 1% cholesterol and 10% Linola flaxseed. A high-cholesterol diet resulted in an elevated plasma cholesterol and triglyceride levels compared to the control animals. Aortic sections from rabbits fed Linola had lower deposits of foamy cells than those from rabbits fed W86. A potassium-induced and phenylephrine-induced contractile response was enhanced by a high-cholesterol diet and not influenced by the W86 or Linola flaxseed. Pretreatment of the aortic rings with nitro-L-arginine methyl ester resulted in a concentration-dependent tendency to increase the reaction amplitude in the control and high-cholesterol diet groups but not the flaxseed groups. Linola flaxseed with a low ALA content more effectively reduced the atherosclerosis progression compared with the W86 flaxseed with a high concentration of stable ALA. Aorta contractility studies suggested that flaxseed ameliorated an increased contractility in hypercholesterolemia but had little or no impact on NO synthesis in the vascular wall. MDPI 2021-03-04 /pmc/articles/PMC8001360/ /pubmed/33806676 http://dx.doi.org/10.3390/foods10030534 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Bujok, Jolanta
Miśta, Dorota
Wincewicz, Edyta
Króliczewska, Bożena
Dzimira, Stanisław
Żuk, Magdalena
Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties
title Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties
title_full Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties
title_fullStr Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties
title_full_unstemmed Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties
title_short Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties
title_sort atherosclerosis development and aortic contractility in hypercholesterolemic rabbits supplemented with two different flaxseed varieties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001360/
https://www.ncbi.nlm.nih.gov/pubmed/33806676
http://dx.doi.org/10.3390/foods10030534
work_keys_str_mv AT bujokjolanta atherosclerosisdevelopmentandaorticcontractilityinhypercholesterolemicrabbitssupplementedwithtwodifferentflaxseedvarieties
AT mistadorota atherosclerosisdevelopmentandaorticcontractilityinhypercholesterolemicrabbitssupplementedwithtwodifferentflaxseedvarieties
AT wincewiczedyta atherosclerosisdevelopmentandaorticcontractilityinhypercholesterolemicrabbitssupplementedwithtwodifferentflaxseedvarieties
AT kroliczewskabozena atherosclerosisdevelopmentandaorticcontractilityinhypercholesterolemicrabbitssupplementedwithtwodifferentflaxseedvarieties
AT dzimirastanisław atherosclerosisdevelopmentandaorticcontractilityinhypercholesterolemicrabbitssupplementedwithtwodifferentflaxseedvarieties
AT zukmagdalena atherosclerosisdevelopmentandaorticcontractilityinhypercholesterolemicrabbitssupplementedwithtwodifferentflaxseedvarieties