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Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comp...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001487/ https://www.ncbi.nlm.nih.gov/pubmed/33803398 http://dx.doi.org/10.3390/antiox10030418 |
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author | Morazzoni, Paolo Vanzani, Paola Santinello, Sandro Gucciardi, Antonina Zennaro, Lucio Miotto, Giovanni Ursini, Fulvio |
author_facet | Morazzoni, Paolo Vanzani, Paola Santinello, Sandro Gucciardi, Antonina Zennaro, Lucio Miotto, Giovanni Ursini, Fulvio |
author_sort | Morazzoni, Paolo |
collection | PubMed |
description | A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at n = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to n = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low n and the corresponding increase of species at high n. |
format | Online Article Text |
id | pubmed-8001487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80014872021-03-28 Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization Morazzoni, Paolo Vanzani, Paola Santinello, Sandro Gucciardi, Antonina Zennaro, Lucio Miotto, Giovanni Ursini, Fulvio Antioxidants (Basel) Article A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at n = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to n = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low n and the corresponding increase of species at high n. MDPI 2021-03-09 /pmc/articles/PMC8001487/ /pubmed/33803398 http://dx.doi.org/10.3390/antiox10030418 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Morazzoni, Paolo Vanzani, Paola Santinello, Sandro Gucciardi, Antonina Zennaro, Lucio Miotto, Giovanni Ursini, Fulvio Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization |
title | Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization |
title_full | Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization |
title_fullStr | Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization |
title_full_unstemmed | Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization |
title_short | Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization |
title_sort | grape seeds proanthocyanidins: advanced technological preparation and analytical characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001487/ https://www.ncbi.nlm.nih.gov/pubmed/33803398 http://dx.doi.org/10.3390/antiox10030418 |
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