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Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization

A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comp...

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Autores principales: Morazzoni, Paolo, Vanzani, Paola, Santinello, Sandro, Gucciardi, Antonina, Zennaro, Lucio, Miotto, Giovanni, Ursini, Fulvio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001487/
https://www.ncbi.nlm.nih.gov/pubmed/33803398
http://dx.doi.org/10.3390/antiox10030418
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author Morazzoni, Paolo
Vanzani, Paola
Santinello, Sandro
Gucciardi, Antonina
Zennaro, Lucio
Miotto, Giovanni
Ursini, Fulvio
author_facet Morazzoni, Paolo
Vanzani, Paola
Santinello, Sandro
Gucciardi, Antonina
Zennaro, Lucio
Miotto, Giovanni
Ursini, Fulvio
author_sort Morazzoni, Paolo
collection PubMed
description A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at n = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to n = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low n and the corresponding increase of species at high n.
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spelling pubmed-80014872021-03-28 Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization Morazzoni, Paolo Vanzani, Paola Santinello, Sandro Gucciardi, Antonina Zennaro, Lucio Miotto, Giovanni Ursini, Fulvio Antioxidants (Basel) Article A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at n = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to n = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low n and the corresponding increase of species at high n. MDPI 2021-03-09 /pmc/articles/PMC8001487/ /pubmed/33803398 http://dx.doi.org/10.3390/antiox10030418 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Morazzoni, Paolo
Vanzani, Paola
Santinello, Sandro
Gucciardi, Antonina
Zennaro, Lucio
Miotto, Giovanni
Ursini, Fulvio
Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
title Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
title_full Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
title_fullStr Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
title_full_unstemmed Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
title_short Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization
title_sort grape seeds proanthocyanidins: advanced technological preparation and analytical characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001487/
https://www.ncbi.nlm.nih.gov/pubmed/33803398
http://dx.doi.org/10.3390/antiox10030418
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